Information on EC 1.1.1.71 - alcohol dehydrogenase [NAD(P)+]

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.1.1.71
-
RECOMMENDED NAME
GeneOntology No.
alcohol dehydrogenase [NAD(P)+]
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a primary alcohol + NAD(P)+ = an aldehyde + NAD(P)H + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
alcohol:NAD(P)+ oxidoreductase
Reduces aliphatic aldehydes of carbon chain length from 2 to 14, with greatest activity on C4, C6 and C8 aldehydes; also reduces retinal to retinol.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-10-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strains NCIB 8250 and HO1-N
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Manually annotated by BRENDA team
strain NCIMB 3610
-
-
Manually annotated by BRENDA team
strain NCIMB 3610
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain ML30
-
-
Manually annotated by BRENDA team
strain ML30
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain IFO 3426
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-
Manually annotated by BRENDA team
strain IFO 3426
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
var. bosis
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-
Manually annotated by BRENDA team
strains NCIMB 10548 and 196A
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-
Manually annotated by BRENDA team
NCIMB 9494
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-
Manually annotated by BRENDA team
NCIMB 9494
-
-
Manually annotated by BRENDA team
strain NCIMB 13259
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-
Manually annotated by BRENDA team
strain NCIMB 13259
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-
Manually annotated by BRENDA team
strain KGX 39
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-
Manually annotated by BRENDA team
strain KGX 39
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-
Manually annotated by BRENDA team
bifunctional enzyme, C-terminal section belongs to the iron-containing alcohol dehydrogenase family, N-terminal section belongs to the aldehyde dehydrogenase family
UniProt
Manually annotated by BRENDA team
D273-10B
-
-
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
strain 4018
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-
Manually annotated by BRENDA team
strain 4018
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-
Manually annotated by BRENDA team
bifunctional enzyme, C-terminal section belongs to the iron-containing alcohol dehydrogenase family, N-terminal section belongs to the aldehyde dehydrogenase family
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S,5S)-2,5-hexanediol + NADP+
? + NADPH + H+
show the reaction diagram
(S)-2-butanol + NADP+
butanone + NADPH
show the reaction diagram
-
196% relative activity as compared to 2-propanol
-
-
?
(S)-2-butanol + NADP+
butanone + NADPH + H+
show the reaction diagram
-
196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol
-
-
?
1,3-butanediol + NADP+
? + NADPH + H+
show the reaction diagram
-
91% relative activity as compared to 2-propanol
-
-
?
1-butanol + NADP+
1-butanal + NADPH + H+
show the reaction diagram
-
-
-
?
1-butanol + NADP+
butanal + NADPH
show the reaction diagram
-
80% relative activity as compared to 2-propanol
-
-
?
1-butanol + NADP+
butanal + NADPH + H+
show the reaction diagram
1-pentanol + NADP+
pentanal + NADPH + H+
show the reaction diagram
1-phenylethanol + NADP+
1-phenylethanone + NADPH + H+
show the reaction diagram
35% of the activtiy with 1-propanol
-
-
?
1-phenylethanol + NADP+
acetophenone + NADPH + H+
show the reaction diagram
35% compared to activity with 1-propanol
-
-
?
1-phenylmethanol + NADP+
benzaldehyde + NADPH + H+
show the reaction diagram
-
180% relative activity as compared to 2-propanol
-
-
?
1-propanol + NAD+
propanal + NADH + H+
show the reaction diagram
dual cofactor dependency, NAD+ shows 60% of the activity with NADP+
-
-
?
1-propanol + NADP+
propanal + NADPH + H+
show the reaction diagram
2,3-pentanedione + NADPH + H+
? + NADP+
show the reaction diagram
2-butanol + NADP+
2-butanone + NADPH + H+
show the reaction diagram
43% of the activity compared to ethanol
-
-
-
2-butanol + NADP+
butanone + NADPH + H+
show the reaction diagram
2-hydroxy-3-methoxybenzaldehyde + NADPH + H+
2-hydroxy-3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
?
2-methoxybenzaldehyde + NADPH + H+
2-methoxybenzalcohol + NADP+
show the reaction diagram
highest activity
-
-
?
2-pentanol + NADP+
2-pentanone + NADPH + H+
show the reaction diagram
-
67% relative activity as compared to 2-propanol
-
-
?
2-pentanol + NADP+
pentan-2-one + NADPH + H+
show the reaction diagram
-
67% of the activity with 2-propanol
-
-
?
2-propanol + NADP+
acetone + NADPH
show the reaction diagram
2-propanol + NADP+
acetone + NADPH + H+
show the reaction diagram
3,4-dimethoxybenzaldehyde + NADPH + H+
3,4-dimethoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
?
3-methyl-2-pentanone + NADPH + H+
? + NADP+
show the reaction diagram
-
13% relative activity as compared to pyruvaldehyde
-
-
?
3-methylbutanal + NADP+
? + NADPH + H+
show the reaction diagram
about% of the activity with 1-butanol
-
-
?
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzalcohol + NADP+
show the reaction diagram
-
-
-
?
acetaldehyde + NADH + H+
ethanol + NAD+
show the reaction diagram
acetaldehyde + NADPH + H+
ethanol + NADP+
show the reaction diagram
all-trans-retinol + NADP+
all-trans-retinal + NADPH + H+
show the reaction diagram
-
-
-
r
allyl alcohol + NADP+
acrylaldehyde + NADPH
show the reaction diagram
benzaldehyde + NAD(P)H
benzyl alcohol + NAD(P)+
show the reaction diagram
-
-
-
?
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
show the reaction diagram
-
-
-
?
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
-
-
-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
show the reaction diagram
butan-1,4-diol + NADP+
butandialdehyde + NADPH
show the reaction diagram
-
29% of activity with hexan-1-ol
-
r
butan-1-ol + NADP+
butanal + NADPH
show the reaction diagram
butanal + NAD(P)H + H+
butan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
cellobiose + NADP+
? + NADPH + H+
show the reaction diagram
cinnamaldehyde + NADPH + H+
cinnamyl alcohol + NADP+
show the reaction diagram
-
-
-
?
cis-2-butenol + NAD(P)H
trans-2-butenal + NAD(P)+
show the reaction diagram
cis-4-heptenal + NAD(P)+
cis-4-heptenol + NAD(P)H
show the reaction diagram
-
-
-
?
cis-hex-3-en-1-ol + NADP+
cis-hex-3-en-1-al + NADPH
show the reaction diagram
-
18.7% of activity with hexan-1ol
-
r
citral + NAD(P)H
citrol + NAD(P)+
show the reaction diagram
-
-
-
?
D-arabinose + NADP+
? + NADPH + H+
show the reaction diagram
-
200% relative activity as compared to 2-propanol
-
-
?
D-arabinose + NADP+
D-arabitol + NADPH + H+
show the reaction diagram
-
200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose
-
-
?
D-glucose + NADP+
? + NADPH + H+
show the reaction diagram
D-mannose + NADP+
? + NADPH + H+
show the reaction diagram
-
48% relative activity as compared to 2-propanol
-
-
?
D-ribose + NADP+
? + NADPH + H+
show the reaction diagram
-
35% relative activity as compared to 2-propanol
-
-
?
D-xylose + NADP+
? + NADPH + H+
show the reaction diagram
decan-1-ol + NADP+
decanal + NADPH
show the reaction diagram
-
decrease in enzyme activity with increase in chain length
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r
decanal + NAD(P)H
decan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
dimethylglyoxal + NADPH + H+
? + NADP+
show the reaction diagram
dodecan-1-ol + NADP+
dodecanal + NADPH
show the reaction diagram
-
decrease in enzyme activity with increase in chain length
-
r
dodecanal + NAD(P)H
dodecan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
ethanal + NAD(P)H
ethanol + NAD(P)+
show the reaction diagram
-
-
-
?
ethanediol + NADP+
glyoxal + NADPH
show the reaction diagram
ethanol + NAD+
acetaldehyde + NADH + H+
show the reaction diagram
enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
show the reaction diagram
ethanol + NADP+
ethanal + NADPH + H+
show the reaction diagram
farnesyl aldehyde + NAD(P)H
farnesol + NAD(P)+
show the reaction diagram
-
-
-
?
glycerol + NADP+
glyceraldehyde + NADPH + H+
show the reaction diagram
-
16% relative activity as compared to 2-propanol
-
-
?
glyoxylic acid + NADPH + H+
? + NADP+
show the reaction diagram
-
36% relative activity as compared to pyruvaldehyde
-
-
?
heptan-1-ol + NADP+
heptanal + NADPH
show the reaction diagram
-
-
-
r
heptanal + NAD(P)H
heptan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
hexan-1-ol + NADP+
hexanal + NADPH
show the reaction diagram
hexanal + NADPH + H+
hexanol + NADP+
show the reaction diagram
-
-
-
?
hydrocinnamaldehyde + NADPH + H+
hydrocinnamyl alcohol + NADP+
show the reaction diagram
-
-
-
?
isoamyl alcohol + NADP+
? + NADPH + H+
show the reaction diagram
about 80% of the activity with 1-butanol
-
-
?
L-arabinose + NADP+
? + NADPH + H+
show the reaction diagram
-
17% relative activity as compared to 2-propanol
-
-
?
linolenyl aldehyde + NAD(P)H
linolenol + NAD(P)+
show the reaction diagram
-
-
-
?
linoleyl aldehyde + NAD(P)H
linoleol + NAD(P)+
show the reaction diagram
-
-
-
?
mannitol + NADP+
D-mannose + NADPH
show the reaction diagram
-
6.5% of activity with hexan-1-ol
-
r
methylglyoxal + NADPH
2-hydroxy-propanal + NADPH
show the reaction diagram
-
-
-
?
octan-1-ol + NADP+
octanal + NADPH
show the reaction diagram
-
decrease in enzyme activity with increase in chain length
-
r
octanal + NAD(P)H + H+
octan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
octanal + NADPH + H+
octanol + NADP+
show the reaction diagram
-
-
-
?
oleyl aldehyde + NAD(P)H
oleol + NAD(P)+
show the reaction diagram
-
-
-
?
pentan-1-ol + NADP+
pentanal + NADPH
show the reaction diagram
propan-1-ol + NADP+
propanal + NADPH
show the reaction diagram
pyruvaldehyde + NADPH + H+
? + NADP+
show the reaction diagram
-
-
-
-
?
pyruvaldehyde + NADPH + H+
lactaldehyde + NADP+
show the reaction diagram
retinal + NAD(P)H
retinol + NAD(P)+
show the reaction diagram
-
-
-
?
retinaldehyde + NADH
retinol + NAD+
show the reaction diagram
ribitol + NADP+
D-ribose + NADPH
show the reaction diagram
-
4% of activity with hexan-1-ol
-
r
salicylaldehye + NADPH + H+
salicylalcohol + NADP+
show the reaction diagram
-
-
-
?
tetradecanal + NAD(P)H
tetradecan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
trans-2,cis-6-nonadienal + NAD(P)+
trans-2,cis-6-nonadienol + NAD(P)H
show the reaction diagram
-
-
-
?
trans-2,cis-6-nonadienal + NADPH + H+
trans-2,cis-6-nonadienol + NADP+
show the reaction diagram
-
-
-
?
trans-2-hexenal + NADPH + H+
trans-2-hexenol + NADP+
show the reaction diagram
-
-
-
?
trans-2-nonenal + NADPH
trans-2-nonen-1-ol + NADPH
show the reaction diagram
-
-
-
?
trans-2-nonenal + NADPH + H+
trans-2-nonenol + NADP+
show the reaction diagram
-
-
-
?
trans-2-octenal + NADPH
trans-2-octen-1-ol + NADPH
show the reaction diagram
-
-
-
?
trans-2-pentenol + NAD(P)+
trans-2-pentenal + NAD(P)H
show the reaction diagram
-
-
-
?
trans-trans-2,4-decadienal + NADPH
trans-trans-2,4-decadienol + NADPH
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
retinaldehyde + NADH
retinol + NAD+
show the reaction diagram
-
the enzyme is involved in retinol and vitamin A metabolism, retinoic acid is important in intestinal epithelial cell proliferation and differentiation, the enzyme also plays a role in dietary conversion of beta-carotene to retinol via retinaldehyde
-
-
r
additional information
?
-
Q8H0L8
the enzyme plays an important role in potato defence response to Erwinia carotovora
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD(P)H
NADP+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
optimally active in the presence of 2 M KCl
additional information
-
metal independency is supported by the absence of a significant effect of TsAdh319 preincubation with 10 mM Me2+ for 30 min before measuring the activity in the presence of 1 mM Me2+ or EDTA
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
slight inhibition
4-chloromercuribenzoate
8-hydroxyquinoline
-
1 mM, 100% inhibition with NAD+ as cofactor, 40% with NADP+ as cofactor
acetonitrile
-
complete inhibition in buffer without NaCl and in buffer with 600 mM NaCl
AgNO3
-
1 mM, 100% inhibition
CdCl2
-
1 mM, 24% inhibition with NAD+ as cofactor
Chloroform
-
21% inhibition in buffer without NaCl, 19% inhibition in buffer with 600 mM NaCl
CuSO4
-
1 mM, 75% inhibition with NAD+ as cofactor, 56% with NADP+ as cofactor
Dimethyl formamide
-
87% inhibition in buffer without NaCl, 59% inhibition in buffer with 600 mM NaCl
dimethyl sulfoxide
-
complete inhibition in buffer without NaCl, 60% inhibition in buffer with 600 mM NaCl
ethyl acetate
-
complete inhibition in buffer without NaCl, 67% inhibition in buffer with 600 mM NaCl
Fe2+
-
10 mM, 74% of initial activity
FeSO4
HgCl2
-
1 mM, 100% inhibition
iodoacetamide
-
1 mM, complete inhibition
iodoacetic acid
-
1 mM, 29% inhibition with NAD+ as cofactor, 80% with NADP+ as cofactor
methanol
-
75% inhibition in buffer without NaCl, 81% inhibition in buffer with 600 mM NaCl
Mg2+
-
10 mM, 78% of initial activity
n-decane
-
9% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl
N-ethylmaleimide
n-hexane
-
40% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl
o-phenanthroline
p-hydroxymercuribenzoate
-
strong inhibition
Phenobarbital
-
0.5 mM, 30% inhibition
pyrazole
-
0.5 mM, 35% inhibition
Sodium azide
-
10 mM, 40% inhibition
Sodium glycocholate
-
-
Tween 80
-
-
Zn2+
-
10 mM, 79% of initial activity
ZnSO4
-
10 mM, 21% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cysteine
-
-
methyl jasmonate
-
salicylic acid
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13.1
(2S,5S)-2,5-hexanediol
-
60C, pH 10.5
6.7
1-butanol
pH 10.0, temperature not specified in the publication
21.4
1-propanol
1.5
2,3-Pentanedione
-
60C, pH 7.5
4.8
2-Methoxybenzaldehyde
pH 6.5, 30C
168
2-propanol
24.1
4-methoxybenzaldehyde
pH 6.5, 30C
1.3 - 9.2
acetaldehyde
0.08
all-trans-retinal
pH 7.4, 37C
0.4
all-trans-retinol
pH 7.4, 37C
150
benzyl alcohol
-
-
52.7 - 78.6
butan-1-ol
0.006 - 3.97
Butanal
54.4
D-xylose
-
60C, pH 10.5; pH 10.5, 60C
44 - 4790
ethanol
1.09
heptan-1-ol
-
-
1.22 - 6.8
hexan-1-ol
5.5
Hexanal
pH 6.5, 30C
0.0107
methylglyoxal
-
-
0.5 - 1060
NAD+
0.024 - 1200
NADH
0.02 - 1
NADP+
0.00079 - 0.32
NADPH
6.2
octan-1-ol
-
-
5.8 - 14.1
pentan-1-ol
17.75
Pyruvaldehyde
6.9
Salicylaldehyde
pH 6.5, 30C
27.1
trans-2-hexenal
pH 6.5, 30C
0.0066
trans-2-nonenal
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.2
(2S,5S)-2,5-hexanediol
Thermococcus sibiricus
-
60C, pH 10.5
1.4
2,3-Pentanedione
Thermococcus sibiricus
-
60C, pH 7.5
0.53
2-propanol
210 - 240
acetaldehyde
38.3 - 50.6
butan-1-ol
19.6 - 26.3
Butanal
0.7
D-xylose
Thermococcus sibiricus
-
60C, pH 10.5; pH 10.5, 60C
13.9 - 35.1
ethanol
38.9
heptan-1-ol
Acinetobacter calcoaceticus
-
-
39.4 - 40.3
hexan-1-ol
220 - 240
NADH
0.45 - 2.02
NADP+
1.51 - 280
NADPH
25.4 - 39.8
octan-1-ol
44.7 - 62.9
pentan-1-ol
2.04
Pyruvaldehyde
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
(2S,5S)-2,5-hexanediol
Thermococcus sibiricus
-
60C, pH 10.5
194816
0.479
1-butanol
Haloferax volcanii
D4GP73
pH 10.0, temperature not specified in the publication
556
1.33
2,3-Pentanedione
Thermococcus sibiricus
-
60C, pH 7.5
1963
0.003 - 0.0032
2-propanol
682
0.744 - 150
acetaldehyde
90
0.013
D-xylose
Thermococcus sibiricus
-
60C, pH 10.5
115
0.07
ethanol
Haloferax volcanii
D4GP73
pH 10.0, temperature not specified in the publication
69
1300 - 2700
NADH
8
12.39 - 101
NADP+
10
75.5 - 3700
NADPH
5
0.11
Pyruvaldehyde
Thermococcus sibiricus
-
60C, pH 7.5
5610
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.025
-
wild-type, cofoactor NADPH, pH 7.6, 55C
0.052
-
mutant P704L/H734R, cofoactor NADH, pH 7.6, 55C
0.12
-
mutant P704L/H734R, cofoactor NADPH, pH 7.6, 55C
2.7
-
wild-type, cofoactor NADH, pH 7.6, 55C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
-
aldehyde reduction
6.3
-
reduction of retinal, sharp decrease above pH 6.3 and below pH 6.0
6.5
-
strain B593, butanal reduction
7.4
-
assay at
8.6
-
cofactor NADP+
9.5
-
ethanol oxidation, cofactor NAD+
10.5
-
assay at, oxidation of alcohols
10.7
-
hexan-1-ol oxidation
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
-
aldehyde reduction, cofactor NADH, NADP dependent activity occurs only at pHs below 7
6 - 9
-
strain B592
6.4 - 7.15
7 - 10.5
-
ethanol oxidation, cofactor NAD+
9
-
strain B592, gradual increase up to pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
55 - 60
oxidation of alcohol
70
-
oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl
83
-
oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 80
40C: about 50% of maximal activity, 80C: about 70% of maximal activity
50 - 75
-
50C: about 50% of maximal activity, 75C: about 60% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl
65 - 86
-
65C: about 50% of maximal activity, 86C: about 50% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl
70 - 95
70C: 60% of maximal activity, 95C: 70% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26200
-
2 * 26200, calculated from sequence
28700
-
2 * 28700, SDS-PAGE
36500
-
1 * 36500, 2-D gel electrophoresis
37000
SDS-PAGE
37300
x * 37300, calculated and SDS-PAGE
37800
4 * 37800, SDS-PAGE
40300
-
4 * 40300, SDS-PAGE
41600
x * 41600, His-tagged protein, SDS-PAGE, mass spectrometry
43500
-
1 * 43500, SDS-PAGE
56000 - 60000
-
gel filtration
132000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2.15 A resolution, tetramer of 222 symmetry, the monomer is composed of a cofactor-binding domain and a catalytic domain, it contains a single zinc atom in the putative active side, the tetramer is composed of two dimers
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2.05 A resolution, tetramer of 222 symmetry, the monomer is composed of a cofactor-binding domain and a catalytic domain, it contains a single zinc atom in the putative active side, the tetramer is composed of two dimers
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hanging-drop vapour-diffusion method using 25% (w/v) polyethylene glycol 3350 pH 7.5 as precipitant; to 1.68 A resolution, crystals belong to space group I222, with unit-cell parameters a = 55.63, b = 83.25, c = 120.75 A
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
most stable when stored at pH 8.0 with 2 and 3 M KCl
726769
8 - 9.8
-
-
287280
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
no loss of activity after 15 min, complete loss of activity at 60C
70
-
1.5 h, no loss of activity; 2 h, 10% loss of activity
100
-
half-life 1 h; half-life: 1 h
102
-
Tm-value in presence of 1 M NaCl
104
-
Tm-value in absence of NaCl
additional information
-
thermal denaturation is fully irreversible and follows first-order kinetics
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
most stable when stored at pH 8.0 with 2 M and 3 M KCl
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetonitrile
dimethyl sulfoxide
Glycerol
Methanol
tetrahydrofuran
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 30 days, 80% residual activity
-20C, 30 days, enzyme retains 80% of its original activity
-20C, crude enzyme retains half of its original activity following incubation at -20C for 75 days and almost one third of its original activity following incubation for 42 days
-20C, retains approximately 80% of its original activity after 10 days
4C, inactive within 10 days
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulose, Sephadex G-100
-
ammonium sulfate, DEAE-Sephadex A-50, hydroxyapatite, Sephadex G-200
-
ammonium sulfate, DEAE-Sephadex, Sephadex G-200, 55C for 6 min
-
chromatofocusing, reactive red
-
DEAE-Sephacel, ammonium sulfate, Sephacryl S300HR, Phenyl-Sepharose, Red A
-
DEAE-Sephacel, CM-cellulose, Sephacryl S300HR, Phenyl-Sepharose, Blue A/DEAE-Sephacel
-
isolation of a gene, that is induced after culture filtrate treatment from Erwinia carotovora
purified in one step by immobilised Ni-affinity chromatography
purified in one step by immobilized metal-affinity chromatography
strain B592, DEAE-cellulose, hydroxyapatite, Sephacryl S-300, Matrex Gel Red A
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a hexahistidine-tagged recombinant version of the enzyme is heterologously overexpressed in Haloferax volcanii
expressed in Escherichia coli as a fusion protein with (His)6-tag and 13 amino acid linker at the N-terminal
-
expressed in Escherichia coli; expression in Escherichia coli
-
expression in Escherichia coli
expression in insect SF9 cells
expresssion in Haloferax volcanii
homologously expressed
overexpression in Escherichia coli as a His-tagged protein
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
P704L/H734R
-
the ethanol tolerant phenotype of Clostridium thermocellum is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE). The mutant displays a complete loss of NADH-dependent activity with concomitant acquisition of NADPH-dependent activity. The reduction in specific activity with respect to NADH is far greater (about 25fold less activity) than the increase with respect to NADPH. Although total ADH activity dropps by 25fold, ethanol production does not drop significantly between strains under these conditions