Information on EC 1.1.1.71 - alcohol dehydrogenase [NAD(P)+]

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.1.1.71
-
RECOMMENDED NAME
GeneOntology No.
alcohol dehydrogenase [NAD(P)+]
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a primary alcohol + NAD(P)+ = an aldehyde + NAD(P)H + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
alcohol:NAD(P)+ oxidoreductase
Reduces aliphatic aldehydes of carbon chain length from 2 to 14, with greatest activity on C4, C6 and C8 aldehydes; also reduces retinal to retinol.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-10-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strains NCIB 8250 and HO1-N
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
var. bosis
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Manually annotated by BRENDA team
strains NCIMB 10548 and 196A
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Manually annotated by BRENDA team
NCIMB 9494
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Manually annotated by BRENDA team
NCIMB 9494
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
bifunctional enzyme, C-terminal section belongs to the iron-containing alcohol dehydrogenase family, N-terminal section belongs to the aldehyde dehydrogenase family
UniProt
Manually annotated by BRENDA team
D273-10B
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
bifunctional enzyme, C-terminal section belongs to the iron-containing alcohol dehydrogenase family, N-terminal section belongs to the aldehyde dehydrogenase family
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1R)-1-hydroxy-1-phenylpropan-2-one + NADPH + H+
(1R)-1-phenylpropane-1,2-diol + NADP+
show the reaction diagram
-
-
-
-
?
(2R)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+
(2R)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+
show the reaction diagram
-
-
-
-
?
(2S)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+
(2S)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+
show the reaction diagram
-
-
-
-
?
(2S,5S)-2,5-hexanediol + NADP+
? + NADPH + H+
show the reaction diagram
(R)-2-hydroxypropiophenone + NADPH + H+
?
show the reaction diagram
-
-
-
-
r
(RS)-1-phenylethanol + NAD+
1-phenylethanone + NADH + H+
show the reaction diagram
-
100% activity
-
-
?
(RS)-1-phenylethanol + NADP+
1-phenylethanone + NADPH + H+
show the reaction diagram
-
100% activity
-
-
?
(S)-2-butanol + NADP+
butanone + NADPH
show the reaction diagram
-
196% relative activity as compared to 2-propanol
-
-
?
(S)-2-butanol + NADP+
butanone + NADPH + H+
show the reaction diagram
-
196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol
-
-
?
(S)-2-hydroxypropiophenone + NADPH + H+
?
show the reaction diagram
-
-
-
-
r
1,3-butanediol + NADP+
? + NADPH + H+
show the reaction diagram
-
91% relative activity as compared to 2-propanol
-
-
?
1-(2-bromophenyl)ethanol + NAD(P)+
1-(2-bromophenyl)ethanone + NAD(P)H + H+
show the reaction diagram
-
11.3% activity compared to (RS)-1-phenylethanol
-
-
?
1-(2-chlorophenyl)ethanol + NAD(P)+
1-(2-chlorophenyl)ethanone + NAD(P)H + H+
show the reaction diagram
-
16.4% activity compared to (RS)-1-phenylethanol
-
-
?
1-(3-bromophenyl)ethanol + NAD(P)+
1-(3-bromophenyl)ethanone + NAD(P)H + H+
show the reaction diagram
-
315% activity compared to (RS)-1-phenylethanol
-
-
?
1-(3-chlorophenyl)ethanol + NAD(P)+
1-(3-chlorophenyl)ethanone + NAD(P)H + H+
show the reaction diagram
-
205% activity compared to (RS)-1-phenylethanol
-
-
?
1-(4-bromophenyl)ethanol + NAD(P)+
1-(4-bromophenyl)ethanone + NAD(P)H + H+
show the reaction diagram
-
167% activity compared to (RS)-1-phenylethanol
-
-
?
1-(4-chlorophenyl)ethanone + NAD(P)+
1-(4-chlorophenyl)ethanone + NAD(P)H + H+
show the reaction diagram
-
151% activity compared to (RS)-1-phenylethanol
-
-
?
1-(4-fluorophenyl)ethanol + NAD(P)+
1-(4-fluorophenyl)ethanol + NAD(P)H + H+
show the reaction diagram
-
119% activity compared to (RS)-1-phenylethanol
-
-
?
1-(4-methylphenyl)ethanone + NAD(P)+
1-(4-methylphenyl)ethanol + NAD(P)H + H+
show the reaction diagram
-
189% activity compared to (RS)-1-phenylethanol
-
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?
1-butanol + NAD(P)+
butanal + NAD(P)H + H+
show the reaction diagram
-
2.3% activity compared to (RS)-1-phenylethanol
-
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?
1-butanol + NADP+
1-butanal + NADPH + H+
show the reaction diagram
-
-
-
?
1-butanol + NADP+
butanal + NADPH
show the reaction diagram
-
80% relative activity as compared to 2-propanol
-
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?
1-butanol + NADP+
butanal + NADPH + H+
show the reaction diagram
1-heptanol + NAD(P)+
heptanal + NAD(P)H + H+
show the reaction diagram
-
3.3% activity compared to (RS)-1-phenylethanol
-
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?
1-hexanol + NAD(P)+
hexanal + NAD(P)H + H+
show the reaction diagram
-
2.8% activity compared to (RS)-1-phenylethanol
-
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?
1-octanol + NAD(P)+
octanal + NAD(P)H + H+
show the reaction diagram
-
2.3% activity compared to (RS)-1-phenylethanol
-
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?
1-pentanol + NAD(P)+
pentanal + NAD(P)H + H+
show the reaction diagram
-
2.8% activity compared to (RS)-1-phenylethanol
-
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?
1-pentanol + NADP+
pentanal + NADPH + H+
show the reaction diagram
1-phenyl-1,2-ethanediol + NAD(P)+
?
show the reaction diagram
-
3.4% activity compared to (RS)-1-phenylethanol
-
-
?
1-phenyl-1-propanol + NAD(P)+
1-phenylpropionaldehyde + NAD(P)H + H+
show the reaction diagram
-
13.1% activity compared to (RS)-1-phenylethanol
-
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?
1-phenyl-2-propanol + NAD(P)+
1-phenylpropan-2-one + NAD(P)H + H+
show the reaction diagram
-
39.1% activity compared to (RS)-1-phenylethanol
-
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?
1-phenylethanol + NADP+
1-phenylethanone + NADPH + H+
show the reaction diagram
35% of the activtiy with 1-propanol
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?
1-phenylethanol + NADP+
acetophenone + NADPH + H+
show the reaction diagram
35% compared to activity with 1-propanol
-
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?
1-phenylmethanol + NADP+
benzaldehyde + NADPH + H+
show the reaction diagram
-
180% relative activity as compared to 2-propanol
-
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?
1-propanol + NAD(P)+
propionaldehyde + NAD(P)H + H+
show the reaction diagram
-
1.2% activity compared to (RS)-1-phenylethanol
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?
1-propanol + NAD+
propanal + NADH + H+
show the reaction diagram
1-propanol + NADP+
propanal + NADPH + H+
show the reaction diagram
2,2,2-trifluoroacetophenone + NAD(P)H + H+
2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+
show the reaction diagram
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180% activity compared to acetoin
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?
2,3-pentanedione + NADPH + H+
? + NADP+
show the reaction diagram
2,5-hexandione + NADH + H+
?
show the reaction diagram
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-
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r
2-bromoacetophenone + NAD(P)H + H+
1-(2-bromophenyl)ethanone + NAD(P)+
show the reaction diagram
-
8.7% activity compared to acetoin
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?
2-bromobenzaldehyde + NADPH
2-bromobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
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r
2-bromobenzaldehyde + NADPH + H+
2-bromobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
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?
2-butanol + NAD(P)+
butan-2-one + NAD(P)H + H+
show the reaction diagram
-
22.3% activity compared to (RS)-1-phenylethanol
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?
2-butanol + NADP+
2-butanone + NADPH + H+
show the reaction diagram
43% of the activity compared to ethanol
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-
2-butanol + NADP+
butanone + NADPH + H+
show the reaction diagram
2-chloro-1-phenylethanol + NAD(P)+
2-chloro-1-phenylethan-1-one + NAD(P)H + H+
show the reaction diagram
-
0.3% activity compared to (RS)-1-phenylethanol
-
-
?
2-cyclohexenol + NADP+
2-cyclohexenone + NADH + H+
show the reaction diagram
-
-
-
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r
2-cyclohexenone + NADH + H+
2-cyclohexenol + NADP+
show the reaction diagram
-
-
-
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r
2-fluorobenzaldehyde + NADPH + H+
2-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
2-heptanol + NAD(P)+
heptan-2-one + NAD(P)H + H+
show the reaction diagram
-
61.6% activity compared to (RS)-1-phenylethanol
-
-
?
2-hexanol + NAD(P)+
hexan-2-one + NAD(P)H + H+
show the reaction diagram
-
38.7% activity compared to (RS)-1-phenylethanol
-
-
?
2-hexanone + NAD(P)H + H+
hexan-2-ol + NAD(P)+
show the reaction diagram
-
3.6% activity compared to acetoin
-
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?
2-hydroxy-2-methyl-1-phenylpropan-1-one + NADPH + H+
2-methyl-1-phenylpropan-1,2-diol + NADP+
show the reaction diagram
-
-
-
-
?
2-hydroxy-3-methoxybenzaldehyde + NADPH + H+
2-hydroxy-3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
?
2-methoxybenzaldehyde + NADPH + H+
2-methoxybenzalcohol + NADP+
show the reaction diagram
highest activity
-
-
?
2-methoxybenzaldehyde + NADPH + H+
2-methoxybenzyl alcohol + NADP+
show the reaction diagram
2-methylbenzaldehyde + NADPH + H+
2-methylbenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
2-octanol + NAD(P)+
octan-2-one + NAD(P)H + H+
show the reaction diagram
-
45.8% activity compared to (RS)-1-phenylethanol
-
-
?
2-pentanol + NAD(P)+
pentan-2-one + NAD(P)H + H+
show the reaction diagram
-
30.2% activity compared to (RS)-1-phenylethanol
-
-
?
2-pentanol + NADP+
2-pentanone + NADPH + H+
show the reaction diagram
-
67% relative activity as compared to 2-propanol
-
-
?
2-pentanol + NADP+
pentan-2-one + NADPH + H+
show the reaction diagram
-
67% of the activity with 2-propanol
-
-
?
2-phenylethanol + NAD(P)+
phenylacetaldehyde + NAD(P)H + H+
show the reaction diagram
-
0.3% activity compared to (RS)-1-phenylethanol
-
-
?
2-propanol + NAD(P)+
propan-2-one + NAD(P)H + H+
show the reaction diagram
-
5.3% activity compared to (RS)-1-phenylethanol
-
-
?
2-propanol + NADP+
acetone + NADPH
show the reaction diagram
2-propanol + NADP+
acetone + NADPH + H+
show the reaction diagram
3,4-dimethoxybenzaldehyde + NADPH + H+
3,4-dimethoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
?
3-fluorobenzaldehyde + NADPH
3-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
3-hydroxypropionaldehyde + NADPH + H+
propan-1,3-diol + NADP+
show the reaction diagram
3-methoxybenzaldehyde + NADPH + H+
3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
3-methyl-2-pentanone + NADPH + H+
? + NADP+
show the reaction diagram
-
13% relative activity as compared to pyruvaldehyde
-
-
?
3-methylbenzaldehyde + NADPH + H+
3-methylbenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
3-methylbutanal + NADP+
? + NADPH + H+
show the reaction diagram
about% of the activity with 1-butanol
-
-
?
3-methylcyclohexanol + NADP+
?
show the reaction diagram
-
-
-
-
r
3-methylcyclohexanone + NADH + H+
3-methylcyclohexanol + NAD+
show the reaction diagram
-
-
-
-
r
4-androstene-3,17-dione + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
4-bromobenzaldehyde + NADPH + H+
4-bromobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-fluorobenzaldehyde + NADPH + H+
4-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzalcohol + NADP+
show the reaction diagram
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
show the reaction diagram
4-methylbenzaldehyde + NADPH + H+
4-methylbenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-methylnitrosamino-1-(3-pyridyl)-1-butanone + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
4-methylpentan-2-one + NADPH + H+
4-methylpentan-2-ol + NADP+
show the reaction diagram
-
-
-
-
?
4-nitroacetophenone + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
4-phenyl-2-butanone + NADH + H+
4-phenyl-2-butanol + NAD+
show the reaction diagram
-
-
-
-
r
acetaldehyde + NADH + H+
ethanol + NAD+
show the reaction diagram
acetaldehyde + NADPH
ethanol + NADP+
show the reaction diagram
-
-
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
show the reaction diagram
acetohexamide + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
acetoin + NAD(P)+
2,3-butanediol + NAD(P)H + H+
show the reaction diagram
-
4.5% activity compared to (RS)-1-phenylethanol
-
-
?
acetoin + NAD(P)H + H+
butan-2,3-dione + NAD(P)H + H+
show the reaction diagram
-
100% activity
-
-
?
acetoin + NADPH + H+
?
show the reaction diagram
-
1.9% activity compared to 3-hydroxypropionaldehyde
-
-
?
acetone + NAD(P)H + H+
propan-2-ol + NAD(P)+
show the reaction diagram
-
4.1% activity compared to acetoin
-
-
?
acetone + NADH + H+
propan-2-ol + NAD+
show the reaction diagram
-
-
-
-
r
acetophenone + NADH + H+
1-phenylethan-1-ol + NAD+
show the reaction diagram
-
-
-
-
r
acrolein + NADPH + H+
prop-2-en-1-ol + NADP+
show the reaction diagram
-
41.8% activity compared to 3-hydroxypropionaldehyde
-
-
?
all-trans-retinal + NADPH + H+
all-trans-retinol + NADP+
show the reaction diagram
-
-
-
-
?
all-trans-retinol + NADP+
all-trans-retinal + NADPH + H+
show the reaction diagram
-
-
-
r
allyl alcohol + NADP+
acrylaldehyde + NADPH
show the reaction diagram
benzaldehyde + NAD(P)H
benzyl alcohol + NAD(P)+
show the reaction diagram
-
-
-
?
benzaldehyde + NADPH
benzyl alcohol + NADP+
show the reaction diagram
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
show the reaction diagram
-
-
-
?
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
show the reaction diagram
butan-1,4-diol + NADP+
butandialdehyde + NADPH
show the reaction diagram
-
29% of activity with hexan-1-ol
-
r
butan-1-ol + NADP+
butanal + NADPH
show the reaction diagram
butan-2-ol + NADP+
butan-2-one + NADPH + H+
show the reaction diagram
-
-
-
-
?
butan-2-one + NADPH + H+
butan-2-ol + NADP+
show the reaction diagram
-
-
-
-
?
butanal + NAD(P)H + H+
butan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
butanal + NADPH + H+
1-butanol + NAD(P)+
show the reaction diagram
44% activity compared to pentanal
-
-
?
butyraldehyde + NADPH + H+
butanol + NADP+
show the reaction diagram
cellobiose + NADP+
? + NADPH + H+
show the reaction diagram
cinnamaldehyde + NADPH + H+
cinnamyl alcohol + NADP+
show the reaction diagram
-
-
-
?
cis-2-butenol + NAD(P)H
trans-2-butenal + NAD(P)+
show the reaction diagram
cis-4-cyclopentene-1,3-diol + NADP+
(R)-4-hydroxy-2-cyclopentenone + NADPH + H+
show the reaction diagram
-
-
-
-
?
cis-4-heptenal + NAD(P)+
cis-4-heptenol + NAD(P)H
show the reaction diagram
-
-
-
?
cis-hex-3-en-1-ol + NADP+
cis-hex-3-en-1-al + NADPH
show the reaction diagram
-
18.7% of activity with hexan-1ol
-
r
citral + NAD(P)H
citrol + NAD(P)+
show the reaction diagram
-
-
-
?
cyclohexanol + NAD(P)+
cyclohexanone + NAD(P)H + H+
show the reaction diagram
-
52.3% activity compared to (RS)-1-phenylethanol
-
-
?
cyclohexanol + NADP+
cyclohexanone + NADH + H+
show the reaction diagram
-
-
-
-
r
cyclohexanone + NAD(P)H + H+
cyclohexanol + NAD(P)+
show the reaction diagram
-
19.1% activity compared to acetoin
-
-
?
cyclohexanone + NADH + H+
cyclohexanol + NAD+
show the reaction diagram
-
-
-
-
r
cyclohexanone + NADH + H+
cyclohexanol + NADP+
show the reaction diagram
-
-
-
-
r
D-arabinose + NAD(P)+
?
show the reaction diagram
-
21.4% activity compared to (RS)-1-phenylethanol
-
-
?
D-arabinose + NADP+
? + NADPH + H+
show the reaction diagram
-
200% relative activity as compared to 2-propanol
-
-
?
D-arabinose + NADP+
D-arabitol + NADPH + H+
show the reaction diagram
-
200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose
-
-
?
D-glucose + NADP+
? + NADPH + H+
show the reaction diagram
D-mannose + NADP+
? + NADPH + H+
show the reaction diagram
-
48% relative activity as compared to 2-propanol
-
-
?
D-ribose + NADP+
? + NADPH + H+
show the reaction diagram
-
35% relative activity as compared to 2-propanol
-
-
?
D-xylose + NADP+
? + NADPH + H+
show the reaction diagram
decan-1-ol + NADP+
decanal + NADPH
show the reaction diagram
-
decrease in enzyme activity with increase in chain length
-
r
decanal + NAD(P)H
decan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
dihydroxyacetone + NAD(P)H + H+
?
show the reaction diagram
-
34.8% activity compared to acetoin
-
-
?
dimethylglyoxal + NADPH + H+
? + NADP+
show the reaction diagram
DL-glyceraldehyde + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
dodecan-1-ol + NADP+
dodecanal + NADPH
show the reaction diagram
-
decrease in enzyme activity with increase in chain length
-
r
dodecanal + NAD(P)H
dodecan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
estrone + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
ethanal + NAD(P)H
ethanol + NAD(P)+
show the reaction diagram
-
-
-
?
ethanediol + NADP+
glyoxal + NADPH
show the reaction diagram
ethanol + NAD(P)+
acetaldehyde + NAD(P)H + H+
show the reaction diagram
-
0.5% activity compared to (RS)-1-phenylethanol
-
-
?
ethanol + NAD+
acetaldehyde + NADH + H+
show the reaction diagram
enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
show the reaction diagram
ethanol + NADP+
ethanal + NADPH + H+
show the reaction diagram
ethyl pyruvate + NAD(P)H + H+
?
show the reaction diagram
-
294% activity compared to acetoin
-
-
?
farnesyl aldehyde + NAD(P)H
farnesol + NAD(P)+
show the reaction diagram
-
-
-
?
formaldehyde + NADPH + H+
methanol + NADP+
show the reaction diagram
-
-
-
-
?
furan-2-carbaldehyde + NADPH + H+
(furan-2-yl)methanol + NADP+
show the reaction diagram
-
-
-
-
?
furfural + NADPH + H+
(furan-2-yl)methanol + NAD(P)+
show the reaction diagram
5% activity compared to pentanal
-
-
?
glycerol + NADP+
glyceraldehyde + NADPH + H+
show the reaction diagram
-
16% relative activity as compared to 2-propanol
-
-
?
glycoaldehyde + NADPH + H+
?
show the reaction diagram
5% activity compared to pentanal
-
-
?
glyoxylic acid + NADPH + H+
? + NADP+
show the reaction diagram
-
36% relative activity as compared to pyruvaldehyde
-
-
?
heptan-1-ol + NADP+
heptanal + NADPH
show the reaction diagram
-
-
-
r
heptan-4-one + NADPH + H+
heptan-4-ol + NADP+
show the reaction diagram
-
-
-
-
?
heptanal + NAD(P)H
heptan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
heptanal + NADPH + H+
1-heptanol + NAD(P)+
show the reaction diagram
56% activity compared to pentanal
-
-
?
hexan-1-ol + NADP+
hexaldehyde + NADPH + H+
show the reaction diagram
-
-
-
-
?
hexan-1-ol + NADP+
hexanal + NADPH
show the reaction diagram
hexan-2-one + NADPH + H+
hexan-2-ol + NADP+
show the reaction diagram
-
-
-
-
?
hexanal + NAD(P)H + H+
hexan-1-ol + NAD(P)+
show the reaction diagram
-
12.9% activity compared to acetoin
-
-
?
hexanal + NADPH + H+
1-hexanol + NAD(P)+
show the reaction diagram
62% activity compared to pentanal
-
-
?
hexanal + NADPH + H+
hexan-1-ol + NADP+
show the reaction diagram
-
-
-
-
?
hexanal + NADPH + H+
hexanol + NADP+
show the reaction diagram
-
-
-
?
hydrocinnamaldehyde + NADPH + H+
hydrocinnamyl alcohol + NADP+
show the reaction diagram
-
-
-
?
isoamyl alcohol + NADP+
? + NADPH + H+
show the reaction diagram
about 80% of the activity with 1-butanol
-
-
?
L-arabinose + NAD(P)+
?
show the reaction diagram
-
5.2% activity compared to (RS)-1-phenylethanol
-
-
?
L-arabinose + NADP+
? + NADPH + H+
show the reaction diagram
-
17% relative activity as compared to 2-propanol
-
-
?
linolenyl aldehyde + NAD(P)H
linolenol + NAD(P)+
show the reaction diagram
-
-
-
?
linoleyl aldehyde + NAD(P)H
linoleol + NAD(P)+
show the reaction diagram
-
-
-
?
mannitol + NADP+
D-mannose + NADPH
show the reaction diagram
-
6.5% of activity with hexan-1-ol
-
r
meso-2,3-butanediol + NAD+
?
show the reaction diagram
-
272% activity compared to (RS)-1-phenylethanol
-
-
?
meso-2,3-butanediol + NADP+
?
show the reaction diagram
-
272% activity compared to (RS)-1-phenylethanol
-
-
?
methanol + NAD(P)+
formaldehyde + NAD(P)H + H+
show the reaction diagram
-
0.2% activity compared to (RS)-1-phenylethanol
-
-
?
methyl benzoylformate + NAD(P)H + H+
?
show the reaction diagram
-
67% activity compared to acetoin
-
-
?
methylglyoxal + NADPH
2-hydroxy-propanal + NADPH
show the reaction diagram
-
-
-
?
methylglyoxal + NADPH + H+
?
show the reaction diagram
29% activity compared to pentanal
-
-
?
octan-1-ol + NADP+
octaldehyde + NADPH + H+
show the reaction diagram
-
-
-
-
?
octan-1-ol + NADP+
octanal + NADPH
show the reaction diagram
-
decrease in enzyme activity with increase in chain length
-
r
octan-2-one + NADPH + H+
octan-2-ol + NADP+
show the reaction diagram
-
-
-
-
?
octanal + NAD(P)H + H+
octan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
octanal + NADPH + H+
1-octanol + NAD(P)+
show the reaction diagram
55% activity compared to pentanal
-
-
?
octanal + NADPH + H+
octanol + NADP+
show the reaction diagram
-
-
-
?
oleyl aldehyde + NAD(P)H
oleol + NAD(P)+
show the reaction diagram
-
-
-
?
pentan-1-ol + NADP+
pentaldehyde + NADPH + H+
show the reaction diagram
-
-
-
-
?
pentan-1-ol + NADP+
pentanal + NADPH
show the reaction diagram
pentanal + NADPH + H+
1-pentanol + NAD(P)+
show the reaction diagram
100% activity
-
-
?
phenylacetaldehyde + NAD(P)H + H+
2-phenylethan-1-ol + NAD(P)+
show the reaction diagram
-
18.6% activity compared to acetoin
-
-
?
propan-1-ol + NADP+
propanal + NADPH
show the reaction diagram
propan-1-ol + NADP+
propionaldehyde + NADPH + H+
show the reaction diagram
-
-
-
-
?
propan-2-ol + NADP+
propan-2-one + NADPH + H+
show the reaction diagram
-
-
-
-
?
propanal + NADPH + H+
1-propanol + NAD(P)+
show the reaction diagram
14% activity compared to pentanal
-
-
?
propionaldehyde + NADPH + H+
propanol + NADP+
show the reaction diagram
pyridine-2-carbaldehyde + NADPH + H+
(pyridin-2-yl)methanol + NADP+
show the reaction diagram
-
-
-
-
?
pyruvaldehyde + NADPH + H+
? + NADP+
show the reaction diagram
-
-
-
-
?
pyruvaldehyde + NADPH + H+
lactaldehyde + NADP+
show the reaction diagram
pyruvic aldehyde + NAD(P)H + H+
?
show the reaction diagram
-
328% activity compared to acetoin
-
-
?
rac-1-nonen-4-ol + NADP+
(S)-1-nonen-4-ol + 1-nonen-4-one + NADPH + H+
show the reaction diagram
-
-
-
-
?
rac-1-phenylethanol + NAD(P)+
acetophenone + (R)-1-phenylethanol + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
rac-1-phenylethanol + NADP+
(R)-1-phenylethanol + (S)-1-phenylethanol + NADPH + H+
show the reaction diagram
-
-
-
-
?
retinal + NAD(P)H
retinol + NAD(P)+
show the reaction diagram
-
-
-
?
retinaldehyde + NADH
retinol + NAD+
show the reaction diagram
ribitol + NADP+
D-ribose + NADPH
show the reaction diagram
-
4% of activity with hexan-1-ol
-
r
salicylaldehye + NADPH + H+
salicylalcohol + NADP+
show the reaction diagram
-
-
-
?
tetradecanal + NAD(P)H
tetradecan-1-ol + NAD(P)+
show the reaction diagram
-
-
-
?
trans-2,cis-6-nonadienal + NAD(P)+
trans-2,cis-6-nonadienol + NAD(P)H
show the reaction diagram
-
-
-
?
trans-2,cis-6-nonadienal + NADPH + H+
trans-2,cis-6-nonadienol + NADP+
show the reaction diagram
-
-
-
?
trans-2-hexenal + NADPH + H+
trans-2-hexenol + NADP+
show the reaction diagram
-
-
-
?
trans-2-nonenal + NADPH
trans-2-nonen-1-ol + NADPH
show the reaction diagram
-
-
-
?
trans-2-nonenal + NADPH + H+
trans-2-nonenol + NADP+
show the reaction diagram
-
-
-
?
trans-2-octenal + NADPH
trans-2-octen-1-ol + NADPH
show the reaction diagram
-
-
-
?
trans-2-pentenol + NAD(P)+
trans-2-pentenal + NAD(P)H
show the reaction diagram
-
-
-
?
trans-trans-2,4-decadienal + NADPH
trans-trans-2,4-decadienol + NADPH
show the reaction diagram
-
-
-
?
vanillyl alcohol + NAD+
vanillin + NADH + H+
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(RS)-1-phenylethanol + NAD+
1-phenylethanone + NADH + H+
show the reaction diagram
-
100% activity
-
-
?
(RS)-1-phenylethanol + NADP+
1-phenylethanone + NADPH + H+
show the reaction diagram
-
100% activity
-
-
?
1-(2-bromophenyl)ethanol + NAD(P)+
1-(2-bromophenyl)ethanone + NAD(P)H + H+
show the reaction diagram
-
11.3% activity compared to (RS)-1-phenylethanol
-
-
?
1-(2-chlorophenyl)ethanol + NAD(P)+
1-(2-chlorophenyl)ethanone + NAD(P)H + H+
show the reaction diagram
-
16.4% activity compared to (RS)-1-phenylethanol
-
-
?
1-(3-bromophenyl)ethanol + NAD(P)+
1-(3-bromophenyl)ethanone + NAD(P)H + H+
show the reaction diagram
-
315% activity compared to (RS)-1-phenylethanol
-
-
?
1-(3-chlorophenyl)ethanol + NAD(P)+
1-(3-chlorophenyl)ethanone + NAD(P)H + H+
show the reaction diagram
-
205% activity compared to (RS)-1-phenylethanol
-
-
?
1-(4-bromophenyl)ethanol + NAD(P)+
1-(4-bromophenyl)ethanone + NAD(P)H + H+
show the reaction diagram
-
167% activity compared to (RS)-1-phenylethanol
-
-
?
1-(4-chlorophenyl)ethanone + NAD(P)+
1-(4-chlorophenyl)ethanone + NAD(P)H + H+
show the reaction diagram
-
151% activity compared to (RS)-1-phenylethanol
-
-
?
1-(4-fluorophenyl)ethanol + NAD(P)+
1-(4-fluorophenyl)ethanol + NAD(P)H + H+
show the reaction diagram
-
119% activity compared to (RS)-1-phenylethanol
-
-
?
1-(4-methylphenyl)ethanone + NAD(P)+
1-(4-methylphenyl)ethanol + NAD(P)H + H+
show the reaction diagram
-
189% activity compared to (RS)-1-phenylethanol
-
-
?
1-butanol + NAD(P)+
butanal + NAD(P)H + H+
show the reaction diagram
-
2.3% activity compared to (RS)-1-phenylethanol
-
-
?
1-heptanol + NAD(P)+
heptanal + NAD(P)H + H+
show the reaction diagram
-
3.3% activity compared to (RS)-1-phenylethanol
-
-
?
1-hexanol + NAD(P)+
hexanal + NAD(P)H + H+
show the reaction diagram
-
2.8% activity compared to (RS)-1-phenylethanol
-
-
?
1-octanol + NAD(P)+
octanal + NAD(P)H + H+
show the reaction diagram
-
2.3% activity compared to (RS)-1-phenylethanol
-
-
?
1-pentanol + NAD(P)+
pentanal + NAD(P)H + H+
show the reaction diagram
-
2.8% activity compared to (RS)-1-phenylethanol
-
-
?
1-phenyl-1,2-ethanediol + NAD(P)+
?
show the reaction diagram
-
3.4% activity compared to (RS)-1-phenylethanol
-
-
?
1-phenyl-1-propanol + NAD(P)+
1-phenylpropionaldehyde + NAD(P)H + H+
show the reaction diagram
-
13.1% activity compared to (RS)-1-phenylethanol
-
-
?
1-phenyl-2-propanol + NAD(P)+
1-phenylpropan-2-one + NAD(P)H + H+
show the reaction diagram
-
39.1% activity compared to (RS)-1-phenylethanol
-
-
?
1-propanol + NAD(P)+
propionaldehyde + NAD(P)H + H+
show the reaction diagram
-
1.2% activity compared to (RS)-1-phenylethanol
-
-
?
2,2,2-trifluoroacetophenone + NAD(P)H + H+
2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+
show the reaction diagram
-
180% activity compared to acetoin
-
-
?
2-bromoacetophenone + NAD(P)H + H+
1-(2-bromophenyl)ethanone + NAD(P)+
show the reaction diagram
-
8.7% activity compared to acetoin
-
-
?
2-butanol + NAD(P)+
butan-2-one + NAD(P)H + H+
show the reaction diagram
-
22.3% activity compared to (RS)-1-phenylethanol
-
-
?
2-chloro-1-phenylethanol + NAD(P)+
2-chloro-1-phenylethan-1-one + NAD(P)H + H+
show the reaction diagram
-
0.3% activity compared to (RS)-1-phenylethanol
-
-
?
2-heptanol + NAD(P)+
heptan-2-one + NAD(P)H + H+
show the reaction diagram
-
61.6% activity compared to (RS)-1-phenylethanol
-
-
?
2-hexanol + NAD(P)+
hexan-2-one + NAD(P)H + H+
show the reaction diagram
-
38.7% activity compared to (RS)-1-phenylethanol
-
-
?
2-hexanone + NAD(P)H + H+
hexan-2-ol + NAD(P)+
show the reaction diagram
-
3.6% activity compared to acetoin
-
-
?
2-octanol + NAD(P)+
octan-2-one + NAD(P)H + H+
show the reaction diagram
-
45.8% activity compared to (RS)-1-phenylethanol
-
-
?
2-pentanol + NAD(P)+
pentan-2-one + NAD(P)H + H+
show the reaction diagram
-
30.2% activity compared to (RS)-1-phenylethanol
-
-
?
2-phenylethanol + NAD(P)+
phenylacetaldehyde + NAD(P)H + H+
show the reaction diagram
-
0.3% activity compared to (RS)-1-phenylethanol
-
-
?
2-propanol + NAD(P)+
propan-2-one + NAD(P)H + H+
show the reaction diagram
-
5.3% activity compared to (RS)-1-phenylethanol
-
-
?
acetoin + NAD(P)+
2,3-butanediol + NAD(P)H + H+
show the reaction diagram
-
4.5% activity compared to (RS)-1-phenylethanol
-
-
?
acetoin + NAD(P)H + H+
butan-2,3-dione + NAD(P)H + H+
show the reaction diagram
-
100% activity
-
-
?
acetone + NAD(P)H + H+
propan-2-ol + NAD(P)+
show the reaction diagram
-
4.1% activity compared to acetoin
-
-
?
all-trans-retinal + NADPH + H+
all-trans-retinol + NADP+
show the reaction diagram
-
-
-
-
?
cyclohexanol + NAD(P)+
cyclohexanone + NAD(P)H + H+
show the reaction diagram
-
52.3% activity compared to (RS)-1-phenylethanol
-
-
?
cyclohexanone + NAD(P)H + H+
cyclohexanol + NAD(P)+
show the reaction diagram
-
19.1% activity compared to acetoin
-
-
?
D-arabinose + NAD(P)+
?
show the reaction diagram
-
21.4% activity compared to (RS)-1-phenylethanol
-
-
?
dihydroxyacetone + NAD(P)H + H+
?
show the reaction diagram
-
34.8% activity compared to acetoin
-
-
?
ethanol + NAD(P)+
acetaldehyde + NAD(P)H + H+
show the reaction diagram
-
0.5% activity compared to (RS)-1-phenylethanol
-
-
?
ethyl pyruvate + NAD(P)H + H+
?
show the reaction diagram
-
294% activity compared to acetoin
-
-
?
hexanal + NAD(P)H + H+
hexan-1-ol + NAD(P)+
show the reaction diagram
-
12.9% activity compared to acetoin
-
-
?
L-arabinose + NAD(P)+
?
show the reaction diagram
-
5.2% activity compared to (RS)-1-phenylethanol
-
-
?
meso-2,3-butanediol + NAD+
?
show the reaction diagram
-
272% activity compared to (RS)-1-phenylethanol
-
-
?
meso-2,3-butanediol + NADP+
?
show the reaction diagram
-
272% activity compared to (RS)-1-phenylethanol
-
-
?
methanol + NAD(P)+
formaldehyde + NAD(P)H + H+
show the reaction diagram
-
0.2% activity compared to (RS)-1-phenylethanol
-
-
?
methyl benzoylformate + NAD(P)H + H+
?
show the reaction diagram
-
67% activity compared to acetoin
-
-
?
phenylacetaldehyde + NAD(P)H + H+
2-phenylethan-1-ol + NAD(P)+
show the reaction diagram
-
18.6% activity compared to acetoin
-
-
?
pyruvic aldehyde + NAD(P)H + H+
?
show the reaction diagram
-
328% activity compared to acetoin
-
-
?
retinaldehyde + NADH
retinol + NAD+
show the reaction diagram
-
the enzyme is involved in retinol and vitamin A metabolism, retinoic acid is important in intestinal epithelial cell proliferation and differentiation, the enzyme also plays a role in dietary conversion of beta-carotene to retinol via retinaldehyde
-
-
r
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD(P)H
NADP+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
Ca2+ has an stabilizing effect. With 1.0 mM CaCl2, the enzyme is completely stable at 0C for 2 h and after 3.5 h almost 90% of the initial activity is retained
K+
optimally active in the presence of 2 M KCl
additional information
-
metal independency is supported by the absence of a significant effect of TsAdh319 preincubation with 10 mM Me2+ for 30 min before measuring the activity in the presence of 1 mM Me2+ or EDTA
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-2-hydroxypropiophenone
-
-
2-mercaptoethanol
-
slight inhibition
4-chloromercuribenzoate
8-hydroxyquinoline
-
1 mM, 100% inhibition with NAD+ as cofactor, 40% with NADP+ as cofactor
acetonitrile
-
complete inhibition in buffer without NaCl and in buffer with 600 mM NaCl
AgNO3
-
1 mM, 100% inhibition
CdCl2
-
1 mM, 24% inhibition with NAD+ as cofactor
Chloroform
-
21% inhibition in buffer without NaCl, 19% inhibition in buffer with 600 mM NaCl
CuSO4
-
1 mM, 75% inhibition with NAD+ as cofactor, 56% with NADP+ as cofactor
Cyclohexanol
-
-
Dimethyl formamide
-
87% inhibition in buffer without NaCl, 59% inhibition in buffer with 600 mM NaCl
dimethyl sulfoxide
-
complete inhibition in buffer without NaCl, 60% inhibition in buffer with 600 mM NaCl
ethyl acetate
-
complete inhibition in buffer without NaCl, 67% inhibition in buffer with 600 mM NaCl
FeSO4
HgCl2
-
1 mM, 100% inhibition
iodoacetamide
-
1 mM, complete inhibition
iodoacetic acid
-
1 mM, 29% inhibition with NAD+ as cofactor, 80% with NADP+ as cofactor
methanol
-
75% inhibition in buffer without NaCl, 81% inhibition in buffer with 600 mM NaCl
n-decane
-
9% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl
N-ethylmaleimide
n-hexane
-
40% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl
Ni2+
14% residual activity at 1 mM
o-phenanthroline
p-hydroxymercuribenzoate
-
strong inhibition
Phenobarbital
-
0.5 mM, 30% inhibition
pyrazole
-
0.5 mM, 35% inhibition
Sodium azide
-
10 mM, 40% inhibition
Sodium glycocholate
-
-
Tween 80
-
-
ZnSO4
-
10 mM, 21% inhibition
additional information
-
dithiothreitol and EDTA have no effect on either oxidative or reductive activity
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cysteine
-
-
methyl jasmonate
-
salicylic acid
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13.1
(2S,5S)-2,5-hexanediol
-
60C, pH 10.5
1.6
(R)-2-hydroxypropiophenone
-
at pH 7.5 and 30C
244
(RS)-1-phenylethanol
-
with NAD+ as cosubstrate, at pH 6.0 and 70C
3.6
(S)-2-Hydroxypropiophenone
-
at pH 7.5 and 30C
6.7
1-butanol
pH 10.0, temperature not specified in the publication
21.4
1-propanol
1.5
2,3-Pentanedione
-
60C, pH 7.5
4.8
2-Methoxybenzaldehyde
pH 6.5, 30C
168
2-propanol
24.1
4-methoxybenzaldehyde
pH 6.5, 30C
1.3 - 9.2
acetaldehyde
30.2
acetoin
-
with NADH as cosubstrate, at pH 6.0 and 70C
0.08
all-trans-retinal
pH 7.4, 37C
0.4
all-trans-retinol
pH 7.4, 37C
15.6
benzaldehyde
-
at pH 7.5 and 30C
12.3 - 150
benzyl alcohol
52.7 - 78.6
butan-1-ol
0.006 - 3.97
Butanal
5.2
Cyclohexanol
-
at pH 9.0 and 30C
10.8
cyclohexanone
-
at pH 7.5 and 30C
54.4
D-xylose
-
60C, pH 10.5; pH 10.5, 60C
44 - 4790
ethanol
1.09
heptan-1-ol
-
-
1.22 - 6.8
hexan-1-ol
5.5
Hexanal
pH 6.5, 30C
61.3
meso-2,3-butanediol
-
with NAD+ as cosubstrate, at pH 6.0 and 70C
0.0107
methylglyoxal
-
-
0.089 - 1060
NAD+
0.024 - 1200
NADH
0.02 - 1
NADP+
0.00079 - 0.32
NADPH
6.2
octan-1-ol
-
-
5.8 - 14.1
pentan-1-ol
17.75
Pyruvaldehyde
6.9
Salicylaldehyde
pH 6.5, 30C
27.1
trans-2-hexenal
pH 6.5, 30C
0.0066
trans-2-nonenal
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.2
(2S,5S)-2,5-hexanediol
-
60C, pH 10.5
35.7
(RS)-1-phenylethanol
-
with NAD+ as cosubstrate, at pH 6.0 and 70C
1.4
2,3-Pentanedione
-
60C, pH 7.5
0.53
2-propanol
210 - 240
acetaldehyde
13.7
acetoin
-
with NADH as cosubstrate, at pH 6.0 and 70C
38.3 - 50.6
butan-1-ol
19.6 - 26.3
Butanal
0.7
D-xylose
-
60C, pH 10.5; pH 10.5, 60C
13.9 - 35.1
ethanol
38.9
heptan-1-ol
-
-
39.4 - 40.3
hexan-1-ol
43.8
meso-2,3-butanediol
-
with NAD+ as cosubstrate, at pH 6.0 and 70C
10.4 - 30.8
NAD+
15.6 - 240
NADH
0.45 - 30
NADP+
1.51 - 280
NADPH
25.4 - 39.8
octan-1-ol
44.7 - 62.9
pentan-1-ol
2.04
Pyruvaldehyde
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
(2S,5S)-2,5-hexanediol
-
60C, pH 10.5
0.15
(RS)-1-phenylethanol
-
with NAD+ as cosubstrate, at pH 6.0 and 70C
0.479
1-butanol
pH 10.0, temperature not specified in the publication
1.33
2,3-Pentanedione
-
60C, pH 7.5
0.003 - 0.0032
2-propanol
0.744 - 150
acetaldehyde
0.45
acetoin
-
with NADH as cosubstrate, at pH 6.0 and 70C
0.013
D-xylose
-
60C, pH 10.5
0.07
ethanol
pH 10.0, temperature not specified in the publication
0.71
meso-2,3-butanediol
-
with NAD+ as cosubstrate, at pH 6.0 and 70C
117 - 243
NAD+
149 - 2700
NADH
12.39 - 265
NADP+
75.5 - 3700
NADPH
0.11
Pyruvaldehyde
-
60C, pH 7.5
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16.4
(R)-2-hydroxypropiophenone
-
at pH 7.5 and 30C
11.4
Cyclohexanol
-
at pH 9.0 and 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
-
with acetone, at pH 7.5 and 30C
0.025
-
wild-type, cofoactor NADPH, pH 7.6, 55C
0.05
-
with acetaldehyde, at pH 7.5 and 30C
0.052
-
mutant P704L/H734R, cofoactor NADH, pH 7.6, 55C
0.08
-
with 2,5-hexandione, at pH 7.5 and 30C
0.11
-
with cyclohexanone, at pH 7.5 and 30C
0.12
-
mutant P704L/H734R, cofoactor NADPH, pH 7.6, 55C
0.56
-
with 4-phenyl-2-butanone, at pH 7.5 and 30C
2.7
-
wild-type, cofoactor NADH, pH 7.6, 55C
4.35
-
with acetophenone, at pH 7.5 and 30C
4.89
-
with benzaldehyde, at pH 7.5 and 30C
8.36
-
with cyclohexanol, at pH 10.5 and 30C
9.78
-
with cyclohexanone, at pH 7.5 and 30C
11.58
-
with 3-methylcyclohexanol, at pH 10.5 and 30C
11.7
-
with 3-fluorobenzaldehyde, at pH 7.5 and 30C
12.62
-
with 3-methylcyclohexanone, at pH 7.5 and 30C
17.1
-
with (S)-2-hydroxypropiophenone, at pH 7.5 and 30C
18.83
-
with cyclohexenol, at pH 10.5 and 30C
23.31
-
2-bromobenzaldehyde, at pH 7.5 and 30C
362.6
-
with (R)-2-hydroxypropiophenone, at pH 7.5 and 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
-
reduction reaction
5.7
-
aldehyde reduction
6 - 9.5
-
reduction reaction
6.3
-
reduction of retinal, sharp decrease above pH 6.3 and below pH 6.0
6.5
-
strain B593, butanal reduction
7.4
-
assay at
8.6
-
cofactor NADP+
9
-
oxidation reaction
9.5
-
ethanol oxidation, cofactor NAD+
10 - 11.5
-
oxidation reaction
10.5
-
assay at, oxidation of alcohols
10.7
-
hexan-1-ol oxidation
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
-
aldehyde reduction, cofactor NADH, NADP dependent activity occurs only at pHs below 7
6 - 9
-
strain B592
6 - 8
the enzyme shows around 50% of activity at pH 6.0, pH 6.5, and pH 8.0
6.4 - 7.15
7 - 10.5
-
ethanol oxidation, cofactor NAD+
9
-
strain B592, gradual increase up to pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
55 - 60
oxidation of alcohol
70
-
oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl
83
-
oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 50
30 to 60% of activity is observed between 20 and 30C and 20% activity at 10 and 50C, respectively
40 - 80
40C: about 50% of maximal activity, 80C: about 70% of maximal activity
50 - 75
-
50C: about 50% of maximal activity, 75C: about 60% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl
65 - 86
-
65C: about 50% of maximal activity, 86C: about 50% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl
70 - 95
70C: 60% of maximal activity, 95C: 70% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
digestive tract
Manually annotated by BRENDA team
-
small intestine
Manually annotated by BRENDA team
-
small intestine
Manually annotated by BRENDA team
additional information
-
no or very low expression in lungs, spleen, pancreas, large intestine and eye
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26200
-
2 * 26200, calculated from sequence
27000
-
gel filtration
28700
-
2 * 28700, SDS-PAGE
31000
-
1 * 31000, SDS-PAGE
36000
-
x * 36000, SDS-PAGE
36500
-
1 * 36500, 2-D gel electrophoresis
37000
SDS-PAGE
37300
x * 37300, calculated and SDS-PAGE
37800
4 * 37800, SDS-PAGE
40300
-
4 * 40300, SDS-PAGE
41600
x * 41600, His-tagged protein, SDS-PAGE, mass spectrometry
43500
-
1 * 43500, SDS-PAGE
56000 - 60000
-
gel filtration
132000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2.15 A resolution, tetramer of 222 symmetry, the monomer is composed of a cofactor-binding domain and a catalytic domain, it contains a single zinc atom in the putative active side, the tetramer is composed of two dimers
-
2.05 A resolution, tetramer of 222 symmetry, the monomer is composed of a cofactor-binding domain and a catalytic domain, it contains a single zinc atom in the putative active side, the tetramer is composed of two dimers
-
hanging-drop vapour-diffusion method using 25% (w/v) polyethylene glycol 3350 pH 7.5 as precipitant; to 1.68 A resolution, crystals belong to space group I222, with unit-cell parameters a = 55.63, b = 83.25, c = 120.75 A
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8
-
the enzyme shows half-lives of 60-70 h between pH 5.5 and 8.0 at room temperature
740200
8
most stable when stored at pH 8.0 with 2 and 3 M KCl
726769
8 - 9.8
-
-
287280
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 60
the enzyme is thermally stable when incubated for 30 min between 4 and 40C. Incubation for 30 min at temperatures between 10 and 30 C slightly increases the catalytic activity. Recombinant enzyme is completely inactivated at 50 and 60C
8 - 25
-
the enzyme has a half-life of 80 h at 25C and stability optimum between 8 and 15C in the presence of 0.8 mM CaCl2 (half-life of 130 h)
30
-
no loss of activity after 15 min, complete loss of activity at 60C
70
-
1.5 h, no loss of activity; 2 h, 10% loss of activity
95
-
the highly thermostable enzyme has a half-life of 4.5 h at 95C and 16.2 h at 85C
100
-
half-life 1 h; half-life: 1 h
102
-
Tm-value in presence of 1 M NaCl
104
-
Tm-value in absence of NaCl
additional information
-
thermal denaturation is fully irreversible and follows first-order kinetics
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
most stable when stored at pH 8.0 with 2 M and 3 M KCl
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-propanol
-
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Acetone
-
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
acetonitrile
dimethyl sulfoxide
dimethylfluoride
-
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Ethanol
-
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
ethylacetate
-
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Glycerol
hexane
-
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Methanol
octane
-
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
octanol
-
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
tetrahydrofuran
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 10 mM TEA-HCl buffer pH 7.5, at least 80 days, no loss of activity
-
-20C, 30 days, 80% residual activity
-20C, 30 days, enzyme retains 80% of its original activity
-20C, crude enzyme retains half of its original activity following incubation at -20C for 75 days and almost one third of its original activity following incubation for 42 days
-20C, retains approximately 80% of its original activity after 10 days
4C, inactive within 10 days
8C, 50 mM TEA-HCl buffer pH 7.5, 1 day, 91% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulose, Sephadex G-100
-
ammonium sulfate, DEAE-Sephadex A-50, hydroxyapatite, Sephadex G-200
-
ammonium sulfate, DEAE-Sephadex, Sephadex G-200, 55C for 6 min
-
chromatofocusing, reactive red
-
DEAE-Sephacel, ammonium sulfate, Sephacryl S300HR, Phenyl-Sepharose, Red A
-
DEAE-Sephacel, CM-cellulose, Sephacryl S300HR, Phenyl-Sepharose, Blue A/DEAE-Sephacel
-
HisTrap column chromatography, and Superdex 200 gel filtration
-
isolation of a gene, that is induced after culture filtrate treatment from Erwinia carotovora
Ni-NTA column chromatography and Superdex 200 pg gel filtration
purified in one step by immobilised Ni-affinity chromatography
purified in one step by immobilized metal-affinity chromatography
Q sepharose column chromatography
-
Q Sepharose column chromatography, and Superdex 200 gel filtration
-
strain B592, DEAE-cellulose, hydroxyapatite, Sephacryl S-300, Matrex Gel Red A
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a hexahistidine-tagged recombinant version of the enzyme is heterologously overexpressed in Haloferax volcanii
expressed in Escherichia coli as a fusion protein with (His)6-tag and 13 amino acid linker at the N-terminal
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli M15[pREP4] cells
expressed in Escherichia coli Rosetta(DE3) cells
-
expressed in Escherichia coli; expression in Escherichia coli
-
expressed in Sf9 insect cells
-
expression in Escherichia coli
expression in insect SF9 cells
expresssion in Haloferax volcanii
homologously expressed
overexpression in Escherichia coli as a His-tagged protein
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D194A
the mutation results in substantial catalytic deficiency (2.8% compared to the wild type enzyme)
H363A
the mutant with full activity is not able to grow on butanal-containing medium
P704L/H734R
-
the ethanol tolerant phenotype of Clostridium thermocellum is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE). The mutant displays a complete loss of NADH-dependent activity with concomitant acquisition of NADPH-dependent activity. The reduction in specific activity with respect to NADH is far greater (about 25fold less activity) than the increase with respect to NADPH. Although total ADH activity dropps by 25fold, ethanol production does not drop significantly between strains under these conditions