Information on EC 1.1.1.413 - A-factor type gamma-butyrolactone 1'-reductase (1S-forming)

for references in articles please use BRENDA:EC1.1.1.413
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:


The expected taxonomic range for this enzyme is: Streptomyces virginiae

EC NUMBER
COMMENTARY hide
1.1.1.413
-
RECOMMENDED NAME
GeneOntology No.
A-factor type gamma-butyrolactone 1'-reductase (1S-forming)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a (3R,4R)-3-[(1S)-1-hydroxyalkyl]-4-(hydroxymethyl)oxolan-2-one + NADP+ = a (3R,4R)-3-alkanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(3R,4R)-3-[(1S)-1-hydroxyalkyl]-4-(hydroxymethyl)oxolan-2-one:NADP+ 1'-oxidoreductase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-heptanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+
?
show the reaction diagram
3-hexanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+
?
show the reaction diagram
4-(hydroxymethyl)-3-(6-methylheptanoyl)oxolan-2-one + NADPH + H+
?
show the reaction diagram
4-(hydroxymethyl)-3-nonanoyloxolan-2-one + NADPH + H+
?
show the reaction diagram
-
75.3% activity compared to 6-dehydro-Virginiae butanolide-A
-
-
?
4-(hydroxymethyl)-3-octanoyloxolan-2-one + NADPH + H+
?
show the reaction diagram
-
82.8% activity compared to 6-dehydro-Virginiae butanolide-A
-
-
?
4-(hydroxymethyl)-3-pentanoyloxolan-2-one + NADPH + H+
?
show the reaction diagram
-
65.7% activity compared to 6-dehydro-Virginiae butanolide-A
-
-
?
6-dehydro-Virginiae butanolide-A + NADPH + H+
(2R,3R,6S)-Virginiae butanolide-A + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-dehydro-Virginiae butanolide-A + NADPH + H+
(2R,3R,6S)-Virginiae butanolide-A + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0111
6-dehydro-Virginiae butanolide-A
-
at pH 7.5 and 25°C
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 50
-
there is 80% loss and complete loss of activity after 30 min of incubation at 35°C and at 40 to 50°C, respectively
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-