Information on EC 1.1.1.406 - galactitol 2-dehydrogenase (L-tagatose-forming)

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The expected taxonomic range for this enzyme is: Rhodobacter sphaeroides

EC NUMBER
COMMENTARY hide
1.1.1.406
-
RECOMMENDED NAME
GeneOntology No.
galactitol 2-dehydrogenase (L-tagatose-forming)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
galactitol + NAD+ = D-tagatose + NADH + H+
show the reaction diagram
galactitol + NAD+ = L-tagatose + NADH + H+
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
galactitol:NAD+ 2-oxidoreductase (L-tagatose-forming)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R)-butanol + NAD+
2-butanone + NADH
show the reaction diagram
-
-
-
?
(2S)-butanol + NAD+
2-butanone + NADH
show the reaction diagram
-
-
-
?
(2S,3S)-butanediol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
(S)-1,2,6-hexanetriol + NAD+
1,6-dihydroxy-2-hexanone + NADH
show the reaction diagram
-
-
-
-
r
(S)-1,2-butanediol + NAD+
1-hydroxy-2-butanone + NADH
show the reaction diagram
-
-
-
-
r
(S)-1,2-hexanediol + NAD+
1-hydroxy-2-hexanone + NADH
show the reaction diagram
-
-
-
-
r
(S)-1,2-propanediol + NAD+
hydroxyacetone + NADH
show the reaction diagram
-
-
-
-
r
1,2-(S)-pentanediol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
r
1,2-butanediol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
1,2-hexanediol + NAD+
2-oxohexanol + NADH + H+
show the reaction diagram
1,2-hexanediol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
1,2-pentanediol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
1,2-propanediol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
1-hydroxy-2-butanone + NADH
(S)-1,2-butanediol + NAD+
show the reaction diagram
-
-
-
-
r
2,3-hexandione + NADH
(2R,3S)-hexanediol + NAD+
show the reaction diagram
-
-
-
-
r
2-hexanol + NAD+
2-hexanone + NADH
show the reaction diagram
-
-
-
?
2-propanol + NAD+
acetone + NADH
show the reaction diagram
-
-
-
?
3,4-hexanedione + NADH
? + NAD+
show the reaction diagram
-
-
reaction product is a complex polymer
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r
acetoin + NADH
? + NAD+
show the reaction diagram
-
-
-
?
D-arabitol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
D-ribulose + NADH
? + NAD+
show the reaction diagram
-
-
-
?
D-threitol + NAD+
? + NADH
show the reaction diagram
-
best substrate tested
-
?
D-xylulose + NADH
? + NAD+
show the reaction diagram
-
-
-
?
dihydroxyacetone + NADH
? + NAD+
show the reaction diagram
-
-
-
?
galactitol + NAD+
L-tagatose + NADH
show the reaction diagram
-
-
-
r
galactitol + NAD+
L-tagatose + NADH + H+
show the reaction diagram
glycerol + NAD+
DL-glyceraldehyde + NADH
show the reaction diagram
-
-
-
r
hydroxyacetone + NADH
(S)-1,2-propanediol + NAD+
show the reaction diagram
-
-
-
-
r
L-erythrulose + NADH
? + NAD+
show the reaction diagram
-
-
-
?
L-glucitol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
meso-erythritol + NAD+
? + NADH + H+
show the reaction diagram
xylitol + NAD+
L-xylulose + NADH + H+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-arabitol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
D-ribulose + NADH
? + NAD+
show the reaction diagram
-
-
-
?
D-threitol + NAD+
? + NADH
show the reaction diagram
-
best substrate tested
-
?
D-xylulose + NADH
? + NAD+
show the reaction diagram
-
-
-
?
galactitol + NAD+
L-tagatose + NADH
show the reaction diagram
-
-
-
r
galactitol + NAD+
L-tagatose + NADH + H+
show the reaction diagram
L-erythrulose + NADH
? + NAD+
show the reaction diagram
-
-
-
?
L-glucitol + NAD+
? + NADH
show the reaction diagram
-
-
-
?
xylitol + NAD+
L-xylulose + NADH + H+
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
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1 mM required for optimal activity
Ni2+
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25% activation at 1 mM
additional information
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activity of GatDH is strictly dependent on the presence of divalent metal ions
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
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13% inhibition at 1 mM, restored by addition of 2 mM MgCl2
EDTA
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68% inhibition at 1 mM, restored by addition of 2 mM MgCl2
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5
(S)-1,2,6-hexanetriol
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pH 6.5, 35°C
49
(S)-1,2-butanediol
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pH 6.5, 35°C
1.4
(S)-1,2-hexanediol
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pH 6.5, 35°C
196
(S)-1,2-Propanediol
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pH 6.5, 35°C
0.2
1,2-hexanediol
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26
1-hydroxy-2-butanone
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pH 6.5, 35°C
1.4
2,3-Hexanedione
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pH 6.5, 35°C
4.2
3,4-hexanedione
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pH 6.5, 35°C
85
D-threitol
-
-
48
dihydroxyacetone
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22 - 240
galactitol
202
Hydroxyacetone
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pH 6.5, 35°C
144
L-erythrulose
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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reduction of acetoin
10.5
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1,2-hexanediol oxidation
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26385
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4 * 26385, amino acid sequence calculation
26657
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x * 26657, MALDI-TOF-MS
28000
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4 * 28000, SDS-PAGE
110000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 26657, MALDI-TOF-MS
homotetramer
tetramer
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4 * 28000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme, free or with bound 1,2-pentanediol or meso-erythritol, hanging drop vapour diffusion method, 0.001 ml of 0.45 mM protein with 1 mM NAD+ or NADH, in 20 mM Bis-Tris, pH 6.5, is mixed with 0.001 ml of reservoir solution containing 100 mM MES, pH 5.5-5.9, 200 mM MgCl2, and 10-20% w/v methoxy poly(ethylene glycol), equilibration against 1 ml reservoir solution, 1 week, X-ray diffraction structure determnination and analysis at 1.25-195 A resolution
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
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63% of the reduction activity
389404
9
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81% of the oxidation activity
389404
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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50% loss of activity after 8 days
50
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50% loss of activity after 12 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
sucrose stabilizes enzyme preparation
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 20 mM Bis-Tris, pH 6.5, 1 mM MgCl2, 30% sucrose, stable for 3 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His(6)-tagged GatDH from Escherichia coli strain BL21GOLD(DE3) by use of Co2+-IDA functionalized Fe2O3 nanoparticles, purification and immobilization method, overview
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
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recombinant His6-tagged GatDH from Escherichia coli strain BL21(DE3), by nickel affinity chromatography the tag is cleaved off, followed by dialysis and gel filtration
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to homogeneity, chromatography steps
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli strain BL21GOLD(DE3)
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expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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expression of His6-tagged GatDH in Escherichia coli strain BL21(DE3)
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis