Information on EC 1.1.1.401 - 2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD+)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.401
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RECOMMENDED NAME
GeneOntology No.
2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD+)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-dehydro-3-deoxy-L-rhamnonate + NAD+ = 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-rhamnose degradation III
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SYSTEMATIC NAME
IUBMB Comments
2-dehydro-3-deoxy-L-rhamnonate:NAD+ 4-oxidoreductase
The enzyme, characterized from the bacteria Sphingomonas sp. SKA58 and Sulfobacillus thermosulfidooxidans, is involved in the non-phosphorylative degradation pathway for L-rhamnose. It does not show any detectable activity with NADP+ or with other aldoses.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-butanediol + NAD+
?
show the reaction diagram
2-dehydro-3-deoxy-L-lyxonate + NAD+
2,4-didehydro-3-deoxy-L-lyxonate + NADH + H+
show the reaction diagram
2-dehydro-3-deoxy-L-mannonate + NAD+
2,4-didehydro-3-deoxy-L-mannonate + NADH + H+
show the reaction diagram
2-dehydro-3-deoxy-L-rhamnonate + NAD+
2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-dehydro-3-deoxy-L-rhamnonate + NAD+
2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
118.6% activity at 5 mM; 5 mM, 118% of initial activity
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
5 mM, 73% residual activity; 73.7% residual activity at 5 mM EDTA
EGTA
5 mM, 72% residual activity; 72.9% residual activity at 5 mM EGTA
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.27
2-dehydro-3-deoxy-L-lyxonate
at pH 8.0 and 25C
1.9
2-dehydro-3-deoxy-L-mannonate
at pH 8.0 and 25C
0.646 - 2
2-dehydro-3-deoxy-L-rhamnonate
0.42
NAD+
at pH 9.5 and 50C; pH 9.5, 50C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18
2-dehydro-3-deoxy-L-lyxonate
Sphingomonas sp.
Q1NEI6
at pH 8.0 and 25C
0.012
2-dehydro-3-deoxy-L-mannonate
Sphingomonas sp.
Q1NEI6
at pH 8.0 and 25C
0.86
2-dehydro-3-deoxy-L-rhamnonate
Sphingomonas sp.
Q1NEI6
at pH 8.0 and 25C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.145
2-dehydro-3-deoxy-L-lyxonate
Sphingomonas sp.
Q1NEI6
at pH 8.0 and 25C
14733
0.0063
2-dehydro-3-deoxy-L-mannonate
Sphingomonas sp.
Q1NEI6
at pH 8.0 and 25C
26828
1.35
2-dehydro-3-deoxy-L-rhamnonate
Sphingomonas sp.
Q1NEI6
at pH 8.0 and 25C
4609
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.78
unpurified enzyme, at pH 9.0 and 50C
4.06
after 5.1fold purification, at pH 9.0 and 50C; pH 9.5, 50C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 11.5
little or no activity below pH 7.0, at pH 11.5 the enzme shows 75% of the activity at pH 9.5
8 - 11
the enzyme has no or little activity at pH below 7.0, about 50% activity at pH 8.0, while the enzyme activity at pH 11.0 is about 75% of that obtained at pH 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 80
after 20 min incubation, the enzyme is stable at temperatures below 60C, maintains 53.8% activity at 70C, and is almost completely inactive at 80C
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
20 min, 53% residual activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Q-Sepharose column chromatography
Ni-NTA column chromatography and Superdex 200 gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha cells