Information on EC 1.1.1.378 - L-rhamnose 1-dehydrogenase [NAD(P)+]

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.378
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RECOMMENDED NAME
GeneOntology No.
L-rhamnose 1-dehydrogenase [NAD(P)+]
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-rhamnose + NAD(P)+ = L-rhamnono-1,4-lactone + NAD(P)H + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-rhamnose degradation II
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L-rhamnose degradation III
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degradation of hexoses
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Fructose and mannose metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
L-rhamnose:NAD(P)+ 1-oxidoreductase
The enzyme, which occurs in the bacteria Azotobacter vinelandii and Sphingomonas sp. SKA58, is part of the non-phosphorylative degradation pathway for L-rhamnose. The enzyme differs in cofactor specificity from EC 1.1.1.173, L-rhamnose 1-dehydrogenase, which is specific for NAD+ and EC 1.1.1.377, L-rhamnose 1-dehydrogenase (NADP+).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-fucose + NAD+
?
show the reaction diagram
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the enzyme shows significant preference for NADP+ over NAD+
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?
L-fucose + NADP+
?
show the reaction diagram
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the enzyme shows significant preference for NADP+ over NAD+
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?
L-lyxose + NAD+
?
show the reaction diagram
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the enzyme shows significant preference for NADP+ over NAD+
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?
L-lyxose + NADP+
?
show the reaction diagram
L-mannose + NAD+
?
show the reaction diagram
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the enzyme shows significant preference for NADP+ over NAD+
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?
L-mannose + NADP+
?
show the reaction diagram
L-rhamnose + NAD(P)+
L-rhamno-1,4-lactone + NAD(P)H + H+
show the reaction diagram
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first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway
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?
L-rhamnose + NAD(P)+
L-rhamnono-1,4-lactone + NAD(P)H + H+
show the reaction diagram
L-rhamnose + NAD+
L-rhamno-1,4-lactone + NADH + H+
show the reaction diagram
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the enzyme shows significant preference for NADP+ over NAD+
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?
L-rhamnose + NAD+
L-rhamnono-1,4-lactone + NADH + H+
show the reaction diagram
L-rhamnose + NADP+
L-rhamno-1,4-lactone + NADPH + H+
show the reaction diagram
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the enzyme shows significant preference for NADP+ over NAD+
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?
L-rhamnose + NADP+
L-rhamnono-1,4-lactone + NADPH + H+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-rhamnose + NAD(P)+
L-rhamno-1,4-lactone + NAD(P)H + H+
show the reaction diagram
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first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway
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?
L-rhamnose + NAD(P)+
L-rhamnono-1,4-lactone + NAD(P)H + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
582 - 673
L-fucose
4.96 - 8.43
L-Lyxose
89.3 - 108
L-Mannose
2.34 - 2.61
L-rhamnose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.8 - 9.2
L-fucose
41.3 - 79.7
L-Lyxose
9.4 - 17
L-Mannose
37.2 - 74.8
L-rhamnose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.007 - 0.016
L-fucose
717
4.9 - 16.05
L-Lyxose
1647
0.09 - 0.19
L-Mannose
2102
14.3 - 35.7
L-rhamnose
703
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.15
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pH 9.0, 30C, substrates: L-fucose, NAD+
0.27
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pH 9.0, 30C, substrates: L-fucose, NADP+
0.62
25C, pH not specified in the publication, cell-free extract, NADP+-dependent activity
1.69
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pH 9.0, 30C, substrates: L-mannose, NAD+
3.64
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pH 9.0, 30C, substrates: L-mannose, NADP+
31.7
25C, pH not specified in the publication, purified enzyme, NAD+-dependent activity
39.8
25C, pH not specified in the publication, purified enzyme, NADP+-dependent activity
50.7
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pH 9.0, 30C, substrates: L-lyxose, NAD+
72.1
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pH 9.0, 30C, substrates: L-rhamnose, NAD+
125
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pH 9.0, 30C, substrates: L-lyxose, NADP+
163
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pH 9.0, 30C, substrates: L-rhamnose, NADP+
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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