Information on EC 1.1.1.377 - L-rhamnose 1-dehydrogenase (NADP+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Thermoplasma acidophilum

EC NUMBER
COMMENTARY hide
1.1.1.377
-
RECOMMENDED NAME
GeneOntology No.
L-rhamnose 1-dehydrogenase (NADP+)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-rhamnose + NADP+ = L-rhamnono-1,4-lactone + NADPH + H+
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Fructose and mannose metabolism
-
-
Microbial metabolism in diverse environments
-
-
degradation of hexoses
-
-
SYSTEMATIC NAME
IUBMB Comments
L-rhamnose:NADP+ 1-oxidoreductase
The enzyme from the archaeon Thermoplasma acidophilum is part of the non-phosphorylative degradation pathway for L-rhamnose. The enzyme differs in cofactor specificity from EC 1.1.1.173, L-rhamnose 1-dehydrogenase, which is specific for NAD+.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
G3LGQ3
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
G3LGQ3
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-lyxose + NADP+
?
show the reaction diagram
G3LGQ3
-
-
-
?
L-mannose + NADP+
?
show the reaction diagram
G3LGQ3
-
-
-
?
L-rhamnose + NADP+
L-rhamno-1,4-lactone + NADPH + H+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-rhamnose + NADP+
L-rhamno-1,4-lactone + NADPH + H+
show the reaction diagram
G3LGQ3
first enzyme of the non-phosphorylative L-rhamnose pathway in archaea
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
G3LGQ3
no activity with NAD+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.37
L-Lyxose
G3LGQ3
pH 7.0, 60°C
6.74
L-Mannose
G3LGQ3
pH 7.0, 60°C
0.46
L-rhamnose
G3LGQ3
pH 7.0, 60°C
0.1 - 0.13
NADP+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
23.77
L-Lyxose
Thermoplasma acidophilum
G3LGQ3
pH 7.0, 60°C
14.88
L-Mannose
Thermoplasma acidophilum
G3LGQ3
pH 7.0, 60°C
22.36
L-rhamnose
Thermoplasma acidophilum
G3LGQ3
pH 7.0, 60°C
16.5 - 18.08
NADP+
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17.35
L-Lyxose
Thermoplasma acidophilum
G3LGQ3
pH 7.0, 60°C
1647
2.2
L-Mannose
Thermoplasma acidophilum
G3LGQ3
pH 7.0, 60°C
2102
48.6
L-rhamnose
Thermoplasma acidophilum
G3LGQ3
pH 7.0, 60°C
703
139 - 171
NADP+
10
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16.2
G3LGQ3
pH 7.0, 60°C, substrates: L-mannose + NADP+
35.1
G3LGQ3
pH 7.0, 60°C, substrates: L-lyxose + NADP+
36.2
G3LGQ3
pH 7.0, 60°C, substrates: L-rhamnose + NADP+
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
G3LGQ3
activity towards L-rhamnose
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
G3LGQ3
activity towards L-rhamnose
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31237
G3LGQ3
x * 31237, calculated from sequence4
34000
G3LGQ3
x * 34000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
G3LGQ3
x * 31237, calculated from sequence4; x * 34000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
G3LGQ3