Information on EC 1.1.1.354 - farnesol dehydrogenase (NAD+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.354
-
RECOMMENDED NAME
GeneOntology No.
farnesol dehydrogenase (NAD+)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesol + NAD+ = (2E,6E)-farnesal + NADH + H+
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesol:NAD+ 1-oxidoreductase
The enzyme from the prune mite Carpoglyphus lactis also acts on geraniol with greater activity [cf. EC 1.1.1.347, geraniol dehydrogenase (NAD+)]. Unlike EC 1.1.1.216, farnesol dehydrogenase (NADP+), this enzyme cannot use NADP+ as cofactor.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesol + NAD+
(2E,6E)-farnesal + NADH + H+
show the reaction diagram
3-methyl-2-buten-1-ol + NAD+
3-methyl-2-butenal + NADH + H+
show the reaction diagram
4.3% of the activity compared to geraniol
-
-
?
citronellol + NAD+
?
show the reaction diagram
4.3% of the activity compared to geraniol
-
-
?
geraniol + NAD+
geranial + NADH + H+
show the reaction diagram
geranylgeraniol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
additional information
?
-
straight-chain alcohols such as 1-octanol, 2-buten-1-ol, 1-butanol and ethanol serve as poor substrates
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesol + NAD+
(2E,6E)-farnesal + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.051
geraniol
pH 8.5, 22C
0.0595
NAD+
pH 8.5, 22C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2996
geraniol
Carpoglyphus lactis
B2NI94
pH 8.5, 22C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
58400
geraniol
Carpoglyphus lactis
B2NI94
pH 8.5, 22C
987
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 10.5
pH 8.0: about 75% of maximal activity, pH 10.5: about 35% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 60
5C: about 50% of maximal activity, 60C: about 50% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 40630, calculated from sequence; 1 * 42800, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression of recombinant Arabidopsis farnesol dehydrogenase activity in Saccharomyces cerevisiae strain SM1058 using pCL196 plasmid
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is increased in mutants with T-DNA insertions in the FLDH 5' flanking region indicating that both T-DNA insertions disrupt a cis-acting negative regulatory element in the FLDH promoter
FLDH expression is repressed by exogenous abscisic acid and FLDH expression negatively regulates ABA signaling