Information on EC 1.1.1.347 - geraniol dehydrogenase (NAD+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.347
-
RECOMMENDED NAME
GeneOntology No.
geraniol dehydrogenase (NAD+)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geraniol + NAD+ = geranial + NADH + H+
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta; myrcene degradation
-
-
Biosynthesis of antibiotics
-
-
geraniol and nerol degradation
-
-
Geraniol degradation
-
-
isoprenoid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
geraniol:NAD+ oxidoreductase
The enzyme from the bacterium Castellaniella defragrans is most active in vitro with perillyl alcohol [2]. The enzyme from the prune mite Carpoglyphus lactis also acts (more slowly) on farnesol but not on nerol [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone in Carpoglyphus lactis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesol + NAD+
(2E,6E)-farnesyl aldehyde + NADH + H+
show the reaction diagram
-
50% activity compared to geraniol
-
-
?
(S)-perillyl alcohol + NAD+
? + NADH + H+
show the reaction diagram
(S)-perillyl alcohol + NAD+
perillyl aldehyde + NADH
show the reaction diagram
-
-
-
?
3-methyl-2-buten-1-ol + NAD+
3-methyl-but-2-enal + NADH + H+
show the reaction diagram
-
4.3% activity compared to geraniol
-
-
?
benzyl alcohol + NAD+
?
show the reaction diagram
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
show the reaction diagram
citronellol + NAD+
?
show the reaction diagram
-
-
-
?
citronellol + NAD+
citronellal + NADH + H+
show the reaction diagram
cumic alcohol + NAD+
?
show the reaction diagram
-
-
-
?
geraniol + dichlorophenolindophenol
?
show the reaction diagram
-
-
-
?
geraniol + NAD+
geranial + NADH + H+
show the reaction diagram
geraniol + oxidized dichlorophenolindophenol
geranial + reduced dichlorophenolindophenol
show the reaction diagram
-
-
-
?
nerol + NAD+
?
show the reaction diagram
-
-
-
?
nerol + NAD+
neral + NADH + H+
show the reaction diagram
-
-
-
?
p-cumic alcohol + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geraniol + NAD+
geranial + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
enzyme reduces one molecule of NAD+ to NADH per geraniol molecule provided
additional information
NADPH is ineffective as a cosubstrate
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-cyclohexyl-N-(2-morpholinoethyl)carbodiimide
I80 of less than 34 mM
Diethylpyrocarbonate
I80 of less than 17 mM
EDTA
7 mM, 96% inhibition; 7 mM EDTA inhibits the enzyme by 96%
N-ethylmaleimide
I80 of less than 0.5 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0044 - 0.007
(S)-perillyl alcohol
0.1157 - 0.17
benzyl alcohol
0.0575 - 0.086
citronellol
0.006 - 0.021
cumic alcohol
0.0033 - 0.051
geraniol
0.0595
NAD+
-
in 50 mM Tris-HCl (pH 8.5), at 22°C
0.0232 - 0.045
nerol
0.021
p-cumic alcohol
pH 9.4, 21°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2996
geraniol
Carpoglyphus lactis
-
in 50 mM Tris-HCl (pH 8.5), at 22°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2020 - 3550
(S)-perillyl alcohol
13242
110 - 220
benzyl alcohol
260
50 - 100
citronellol
4512
520 - 940
cumic alcohol
42407
620 - 58800
geraniol
987
310
nerol
Castellaniella defragrans
H1ZV38
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C; pH 9.4, 21°C; recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21°C
2280
520
p-cumic alcohol
Castellaniella defragrans
H1ZV38
pH 9.4, 21°C
42276
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.016
-
crude enzyme, in 50 mM Tris-HCl (pH 8.5), at 22°C
31
-
after 1879fold purification, in 50 mM Tris-HCl (pH 8.5), at 22°C
additional information
the crude cell extract shows a specific activity of 0.056 units/mg, while the 114fold purified enzyme shows a specific activity of 4.33 units/mg at pH 9.4 and 21°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
-
as the pH increases from 7.0 to 7.5, there is a sharp increase in enzyme activity whereas the activity decreases sharply beyond pH 10.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38272
2 * 38272, calculated; 2 * 38272, calculated from amino acid sequence
39000
2 * 39000, SDS-PAGE
40630
-
1 * 40630, calculated from amino acid sequence
42800
-
1 * 42800, active form, SDS-PAGE
52000 - 91000
gel filtration
52000
gel filtration
56200
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
monomer
-
1 * 40630, calculated from amino acid sequence; 1 * 42800, active form, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Toyopearl phenyl-650M column chromatography, Toyopearl HW-50F column chromatography, DEAE-cellulose column chromatography, Blue-Cellulofine column chromatography, MonoQ column chromatography and Superdex 200gel filtration
-
DEAE column chromatography and phenyl-Sepharose 6 gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain BL21 Star(DE3); expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
activitiy is induced during growth with limonene in comparison to growth with acetate; enzyme activity is induced during growth with limonene in comparison to growth with acetate