Information on EC 1.1.1.340 - 1-deoxy-11beta-hydroxypentalenate dehydrogenase

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The expected taxonomic range for this enzyme is: Streptomyces avermitilis

EC NUMBER
COMMENTARY hide
1.1.1.340
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RECOMMENDED NAME
GeneOntology No.
1-deoxy-11beta-hydroxypentalenate dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-deoxy-11beta-hydroxypentalenate + NAD+ = 1-deoxy-11-oxopentalenate + NADH + H+
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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-
neopentalenoketolactone and pentalenate biosynthesis
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-
pentalenolactone biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
1-deoxy-11beta-hydroxypentalenate:NAD+ oxidoreductase
Isolated from the bacterium Streptomyces avermitilis and present in many other Streptomyces species. Part of the pathway for pentalenolactone biosynthesis.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-deoxy-11beta-hydroxypentalenic acid + NAD+
1-deoxy-11-oxopentalenic acid + NADH + H+
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
150fold preference for NAD+ over NADP+
NADP+
150fold preference for NAD+ over NADP+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0065
1-deoxy-11beta-hydroxypentalenic acid
pH 8.0, 22°C
0.025
NAD+
pH 8.0, 22°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.65
1-deoxy-11beta-hydroxypentalenic acid
Streptomyces avermitilis
Q82IY9
pH 8.0, 22°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
and second maximum at pH 11.3
11.3
activity increases from pH 9.0 to pH 11.3, and is 12fold higher at pH 11.3 than at pH 8.0
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30183
x * 30183, MALDI-TOF, recombinant protein including His-tag
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 30183, MALDI-TOF, recombinant protein including His-tag
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli