Information on EC 1.1.1.332 - chanoclavine-I dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.332
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RECOMMENDED NAME
GeneOntology No.
chanoclavine-I dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
chanoclavine-I + NAD+ = chanoclavine-I aldehyde + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
chanoclavine I aldehyde biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
chanoclavine-I:NAD+ oxidoreductase
The enzyme catalyses a step in the pathway of ergot alkaloid biosynthesis in certain fungi.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
chanoclavine-I + NAD+
chanoclavine-I aldehyde + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
5 mM, addition to EDTA-treated enzyme, 31% of control activity
Ni2+
5 mM, addition to EDTA-treated enzyme, 73% of control activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09 - 0.27
chanoclavine-I
0.36 - 1.1
NAD+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.38 - 0.76
chanoclavine-I
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26200
4 * 26200, calculated, 4 * 30000, SDS-PAGE of recombinant His-tagged protein
118000
gel filtration
128000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli