Information on EC 1.1.1.310 - (S)-sulfolactate dehydrogenase

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The expected taxonomic range for this enzyme is: Chromohalobacter salexigens

EC NUMBER
COMMENTARY hide
1.1.1.310
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RECOMMENDED NAME
GeneOntology No.
(S)-sulfolactate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2S)-3-sulfolactate + NAD+ = 3-sulfopyruvate + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sulfolactate degradation I
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Cysteine and methionine metabolism
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SYSTEMATIC NAME
IUBMB Comments
(2S)-sulfolactate:NAD+ oxidoreductase
This enzyme, isolated from the bacterium Chromohalobacter salexigens DSM 3043, acts only on the (S)-enantiomer of 3-sulfolactate. Combined with EC 1.1.1.338, (2R)-3-sulfolactate dehydrogenase (NADP+), it provides a racemase system that converts (2S)-3-sulfolactate to (2R)-3-sulfolactate, which is degraded further by EC 4.4.1.24, (2R)-sulfolactate sulfo-lyase. The enzyme is specific for NAD+.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S)-3-sulfolactate + NAD+
3-sulfopyruvate + NADH + H+
show the reaction diagram
3-sulfopyruvate + NADH + H+
(2S)-3-sulfolactate + NAD+
show the reaction diagram
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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SlcC is not inhibited by pyruvate or oxaloacetate (5 mM), (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7
(2S)-3-sulfolactate
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apparent value, in 25 mM Tris buffer, pH 9.0 and 25C
0.14
3-sulfopyruvate
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apparent value, in 25 mM Tris buffer, pH 9.0 and 25C
2
NAD+
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apparent value, in 25 mM Tris buffer, pH 9.0 and 25C
0.05
NADH
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apparent value, in 25 mM Tris buffer, pH 9.0 and 25C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.2
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crude extracts of acetate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25C
7.4
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crude extracts of sulfolactate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25C
675
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enzyme from sulfolactate-grown cells after 91fold purification, using 3-sulfopyruvate as substrate, at pH 9.0 and 25C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
61000
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native enzyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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at 4C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
at 4C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Mono Q column chromatography, phenyl Superose gel filtration, Superose 12 gel filtration
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