Information on EC 1.1.1.295 - momilactone-A synthase

for references in articles please use BRENDA:EC1.1.1.295
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The expected taxonomic range for this enzyme is: Oryza sativa

EC NUMBER
COMMENTARY hide
1.1.1.295
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RECOMMENDED NAME
GeneOntology No.
momilactone-A synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NAD(P)+ = momilactone A + NAD(P)H + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
momilactone A biosynthesis
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Diterpenoid biosynthesis
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide:NAD(P)+ oxidoreductase
The rice phytoalexin momilactone A is a diterpenoid secondary metabolite that is involved in the defense mechanism of the plant. Momilactone A is produced in response to attack by a pathogen through the perception of elicitor signal molecules such as chitin oligosaccharide, or after exposure to UV irradiation. The enzyme, which catalyses the last step in the biosynthesis of momilactone A, can use both NAD+ and NADP+ but activity is higher with NAD+ [1].
CAS REGISTRY NUMBER
COMMENTARY hide
458569-32-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
isoform MS2 more efficiently catalyzes the final oxidation to produce momilactone A than isoform MS1
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2alpha-hydroxy-ent-cassadiene + NAD+
? + NADH + H+
show the reaction diagram
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?
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NAD+
momilactone A + NADH
show the reaction diagram
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?
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NAD+
momilactone A + NADH + H+
show the reaction diagram
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?
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NADP+
momilactone A + NADPH
show the reaction diagram
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reaction with NADP+ is 70% of the activity with NAD+
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?
oryzalexin D + NAD+
oryzalexin A + NADH + H+
show the reaction diagram
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oxidation of the 7beta-hydroxyl group of oryzalexin D
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
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NADP+
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70% of the activity with NAD+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.036 - 0.9
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
0.06
oryzalexin A
pH 8.0, 30°C; pH 8.0, 30°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08 - 0.4
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
0.02 - 0.023
oryzalexin A
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09 - 2
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide
0.3 - 0.4
oryzalexin A
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
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pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36 - 72
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36°C: about 60% of maximal activity, 72°C: about 60% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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Manually annotated by BRENDA team
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli