Information on EC 1.1.1.294 - chlorophyll(ide) b reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.294
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RECOMMENDED NAME
GeneOntology No.
chlorophyll(ide) b reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
71-hydroxychlorophyllide a + NAD(P)+ = chlorophyllide b + NAD(P)H + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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chlorophyll cycle
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Metabolic pathways
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Porphyrin and chlorophyll metabolism
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chlorophyll metabolism
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SYSTEMATIC NAME
IUBMB Comments
71-hydroxychlorophyllide-a:NAD(P)+ oxidoreductase
This enzyme carries out the first step in the conversion of chlorophyll b to chlorophyll a. It is involved in chlorophyll degradation, which occurs during leaf senescence [3] and it also forms part of the chlorophyll cycle, which interconverts chlorophyll a and b in response to changing light conditions [4,5].
CAS REGISTRY NUMBER
COMMENTARY hide
185915-08-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
line 2-24, albina mutant and wild-type line 3629
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
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rice mutants lacking either NYC1 or NOL are deficient in chlorophyll b reductase activity during leaf senescence. Recombinant NOL enzyme shows in vitro chlorophyll b reductase activity in the absence of NYC1, it is possible that NOL could function independently of NYC1. It is possible that the heterodimer formation of NYC1 and NOL is necessary only under specific developmental conditions such as leaf senescence
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
71-hydroxychlorophyllide a + NADP+
chlorophyllide b + NADPH + H+
show the reaction diagram
chlorophyll b + NADPH
7-hydroxymethyl chlorophyll a + NADP+
show the reaction diagram
chlorophyll b + NADPH
? + NADP+
show the reaction diagram
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?
chlorophyllide b + NADPH + H+
7-hydroxychlorophyllide a + NADP+
show the reaction diagram
zinc (132R)-pheophorbide b + NADPH + H+
?
show the reaction diagram
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zinc (132S)-pheophorbide b is a better substrate than zinc (132R)-pheophorbide b
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?
zinc (132S)-pheophorbide b + NADPH + H+
?
show the reaction diagram
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zinc (132S)-pheophorbide b is a better substrate than zinc (132R)-pheophorbide b
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?
Zn-pheophorbide b + NADPH + H+
?
show the reaction diagram
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?
additional information
?
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chlorophyll b reduction is considered to be an early and obligatory step of chlorophyll b breakdown
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
71-hydroxychlorophyllide a + NADP+
chlorophyllide b + NADPH + H+
show the reaction diagram
chlorophyllide b + NADPH + H+
7-hydroxychlorophyllide a + NADP+
show the reaction diagram
additional information
?
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chlorophyll b reduction is considered to be an early and obligatory step of chlorophyll b breakdown
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
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reaction requires NADPH or NADH. NADH is less effective
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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chlorophyll b reductase decreases during senescence and finally disappears after 8-day-dark incubation
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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NYC1 and NO have enhanced expression in senescing tissues
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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expression of NYC1 during embryo development and regulation, semiquantitative reverse transcription-PCR analysis, overview
Manually annotated by BRENDA team
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innercellular structures of the enzyme wild-type and mutant seeds
Manually annotated by BRENDA team
additional information
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isozymes NOL and NYC1 are differentially expressed in Arabidopsis during development
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
NYC1 is a transmembrane protein with three transmembrane domains
Manually annotated by BRENDA team
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 24 hours
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by centrifugation, sonication, on a nickel column and by ultrafiltration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
coding region of chlorophyll b reductase (NOL) lacking its transit peptide amplified and cloned into pET-30a(+) at NspV and HindIII sites, expressed in Escherichia coli Rosetta DE3
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fusion of the region encoding the putative transit peptide of NOL to the green fluorescent protein (GFP) gene and introduced into the epidermal cells of Allium cepa by particle bombardment
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gene nol, DNA and amino acid determination and anaylsis, phylogenetic tree, promoter analysis, semiquantitative RT-PCR expression analysis, overview; gene nyc1, DNA and amino acid determination and anaylsis, phylogenetic tree, promoter analysis, semiquantitative RT-PCR expression analysis, overview
genes nyc1 and nol, the NYC1 promoter contains a potential abscisic acid-responsive element, semiquantitative reverse transcription-PCR expression analysis of isozymes
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positional cloning of nyc1
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
NYC1 expression is repressed in the abscisic acid-insensitive mutants during embryogenesis
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information