Information on EC 1.1.1.279 - (R)-3-hydroxyacid-ester dehydrogenase

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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

EC NUMBER
COMMENTARY hide
1.1.1.279
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RECOMMENDED NAME
GeneOntology No.
(R)-3-hydroxyacid-ester dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ethyl (R)-3-hydroxyhexanoate + NADP+ = ethyl 3-oxohexanoate + NADPH + H+
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
-
-
-
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reduction
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-
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SYSTEMATIC NAME
IUBMB Comments
ethyl-(R)-3-hydroxyhexanoate:NADP+ 3-oxidoreductase
Also acts on ethyl (R)-3-oxobutanoate and some other (R)-3-hydroxy acid esters. The (R)- symbol is allotted on the assumption that no substituents change the order of priority from O-3 > C-2 > C-4. A subunit of yeast fatty acid synthase EC 2.3.1.86 fatty-acyl-CoA synthase). cf. EC 1.1.1.280 (S)-3-hydroxyacid ester dehydrogenase.
CAS REGISTRY NUMBER
COMMENTARY hide
114705-02-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ethyl 3-oxobutanoate + NADPH
ethyl (R)-3-hydroxybutanoate + NADP+
show the reaction diagram
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-
-
?
ethyl 3-oxohexanoate + NADPH
ethyl (R)-3-hydroxyhexanoate + NADP+
show the reaction diagram
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-
-
?
additional information
?
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-
specific for 3-oxo-esters, no reaction with 3-oxo-acids
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
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specific for
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8-hydroxyquinoline 5-sulfonic acid
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-
o-phenanthroline
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17
ethyl 3-oxobutanoate
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-
2
ethyl 3-oxohexanoate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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rapid decrease of activity above
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
780000 - 800000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oligomer
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x * 210000 + x * 200000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using streptomycin sulfate treatment, Sephadex G-25 filtration, DEAE-Sepharose CL-6B chromatography, Sephadex G-150 filtration, Sepharose 6B filtration and hydroxyapatite chromatography
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