Information on EC 1.1.1.238 - 12beta-hydroxysteroid dehydrogenase

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The expected taxonomic range for this enzyme is: Clostridium paraputrificum

EC NUMBER
COMMENTARY hide
1.1.1.238
-
RECOMMENDED NAME
GeneOntology No.
12beta-hydroxysteroid dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3alpha,7alpha,12beta-trihydroxy-5beta-cholan-24-oate + NADP+ = 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oate + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycocholate metabolism (bacteria)
-
-
SYSTEMATIC NAME
IUBMB Comments
12beta-hydroxysteroid:NADP+ 12-oxidoreductase
Acts on a number of bile acids, both in their free and conjugated forms.
CAS REGISTRY NUMBER
COMMENTARY hide
118390-62-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,12-dioxo-5beta-cholanoate + NADPH
12beta-hydroxy-3-oxo-5beta-cholanoate + NADP+
show the reaction diagram
-
-
-
?
3,7,12-trioxo-5beta-cholanoate + NADPH
12beta-hydroxy-3,7-dioxo-5beta-cholanoate + NADP+
show the reaction diagram
-
-
-
?
3,7,12-trioxotaureate + NADPH
12beta-hydroxy-3,7-dioxotaureate + NADP+
show the reaction diagram
-
-
-
?
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate + NADPH
3alpha,7alpha,12beta-trihydroxy-5beta-cholanoate + NADP+
show the reaction diagram
-
-
-
?
3alpha-hydroxy-12-oxo-5beta-cholanoate + NADPH
3alpha,12beta-dihydroxy-5beta-cholanoate + NADP+
show the reaction diagram
-
reverse reaction 10% the rate of forward reaction
-
r
3alpha-hydroxy-7,12-dioxo-5beta-cholanoate + NADPH
3alpha,12beta-dihydroxy-7-oxo-5beta-cholanoate + NADP+
show the reaction diagram
-
-
-
?
7,12-dioxo-5beta-cholanoate + NADPH
12beta-hydroxy-7-oxo-5beta-cholanoate + NADP+
show the reaction diagram
-
-
-
?
7alpha-hydroxy-3,12-dioxo-5beta-cholanoate + NADPH
7alpha,12beta-dihydroxy-3-oxo-5beta-cholanoate + NADP+
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
specific for, no activity with NADH
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0895
3,12-Dioxo-5beta-cholanoate
-
-
0.171
3,7,12-Trioxo-5beta-cholanoate
-
-
0.142
3,7,12-trioxotaureate
-
-
0.274
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate
-
-
0.141
3alpha-Hydroxy-12-oxo-5beta-cholanoate
-
-
0.144
3alpha-Hydroxy-7,12-dioxo-5beta-cholanoate
-
-
0.0837
7,12-Dioxo-5beta-cholanoate
-
-
0.138
7alpha-Hydroxy-3,12-dioxo-5beta-cholanoate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2 - 4.4
-
depending on substrate
2.2 - 6.4
-
depending on inducer
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
-
oxidation of 12beta-hydroxy bile acids
10
-
reduction of 12-oxo bile acids
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 11
-
about half maximum activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
126000
-
gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
-
most stable
286353
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
45 min, complete inactivation
50
-
10 min, 95% inactivation, 15 min complete inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
most stable in presence of reducing agents e.g. dithiothreitol
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, lyophilized powder, at least 4 weeks
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
potential application in quantification of 12beta-hydroxyl groups in di- and trisubstituted bile acids