Information on EC 1.1.1.231 - 15-hydroxyprostaglandin-I dehydrogenase (NADP+)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.231
-
RECOMMENDED NAME
GeneOntology No.
15-hydroxyprostaglandin-I dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
-
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reduction
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-
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SYSTEMATIC NAME
IUBMB Comments
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate:NADP+ 15-oxidoreductase
Specific for prostaglandin I2.
CAS REGISTRY NUMBER
COMMENTARY hide
79468-49-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
chinese hamster
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-
Manually annotated by BRENDA team
monkey
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+
(5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
show the reaction diagram
4-benzoylpyridine + NADP+
?
show the reaction diagram
-
recombinant forms CHCR1, CHCR2
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-
?
4-nitrobenzaldehyde + NADP+
4-nitrobenzoate + NADPH
show the reaction diagram
6-keto-prostaglandin F1alpha + NADP+
6,15-ketoprostaglandin F2alpha + NADPH
show the reaction diagram
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at 1.8% the reaction rate of prostaglandin I2
-
-
?
menadione + NADP+
?
show the reaction diagram
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more rapid reaction than with prostacyclin
-
-
?
phenanthrene quinone + NADP+
?
show the reaction diagram
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more rapid reaction than with prostacyclin
-
-
?
prostaglandin B1 + NADP+
15-ketoprostaglandin B1 + NADPH + H+
show the reaction diagram
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at 8% the reaction rate of prostaglandin I2
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-
?
prostaglandin E2 + NADP+
15-ketoprostaglandin E2 + NADPH + H+
show the reaction diagram
prostaglandin F2alpha + NAD+
15-ketoprostaglandin F2alpha + NADH + H+
show the reaction diagram
-
-
-
-
?
prostaglandin F2alpha + NADP+
15-ketoprostaglandin F2alpha + NADPH + H+
show the reaction diagram
pyridine-4-carboxaldehyde + NADP+
?
show the reaction diagram
-
recombinant forms CHCR1, CHCR2
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+
(5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
show the reaction diagram
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-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Furosemide
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0667
4-Nitrobenzaldehyde
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0.25
menadione
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-
0.00538 - 0.0134
NADP+
0.0005
Phenanthrene quinone
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0.278 - 0.364
prostaglandin I2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.217
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recombinant enzyme, latanoprost + NADP+
2.17
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recombinant enzyme, acid form of latanoprost + NADP+
6.08
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recombinant enzyme, prostaglandin F2alpha + NAD+
9.8
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recombinant enzyme, prostaglandin E2 + NADP+
24.3
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recombinant enzyme, prostaglandin F2alpha + NADP+
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
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tissue distribution of m-RNA
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
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SDs-PAGE of recombinant enzyme
34000
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SDS-PAGE of recombinant forms CHCR1, CHCR2 including His-tag
56000
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SDS-PAGE of amidinated enzyme
62000
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gel filtration
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no modification
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing: repeatedly, slow loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
stable for months when frozen
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 forms differing due to posttranslational modification at pos. 238
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 enzyme forms CHCR1, CHCR2
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