Information on EC 1.1.1.230 - 3alpha-hydroxyglycyrrhetinate dehydrogenase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY hide
1.1.1.230
-
RECOMMENDED NAME
GeneOntology No.
3alpha-hydroxyglycyrrhetinate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3alpha-hydroxyglycyrrhetinate + NADP+ = 3-oxoglycyrrhetinate + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3alpha-hydroxyglycyrrhetinate:NADP+ 3-oxidoreductase
Highly specific to 3alpha-hydroxy derivatives of glycyrrhetinate and its analogues. Not identical to EC 1.1.1.50 3alpha-hydroxysteroid dehydrogenase (Si-specific).
CAS REGISTRY NUMBER
COMMENTARY hide
114308-07-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gastrointestinal bacteria
of rat caecum
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3alpha-hydroxy-18alpha-glycyrrhetic acid + NADP+
3-keto-18alpha-glycyrrhetic acid + NADPH
show the reaction diagram
-
-
-
r
3alpha-hydroxyglycyrrhetic acid + NADP+
3-ketoglycyrrhetic acid + NADPH
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3alpha-hydroxyglycyrrhetic acid + NADP+
3-ketoglycyrrhetic acid + NADPH
show the reaction diagram
-
oxidation of 3alpha-hydroxy group faster than reduction of 3-keto group
-
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glycyrrhetic acid
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glycyrrhizin
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001
3-Ketoglycyrrhetic acid
-
-
0.002
3alpha-Hydroxyglycyrrhetic acid
-
-
0.00027
NADP+
-
-
0.024
NADPH
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00002
-
enzyme activity in microsomal fraction of liver homogenate
additional information
gastrointestinal bacteria
-
0.002 micromol/min/mg wet weight of gastrointestinal content
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
reduction of 3-keto group
8
-
oxidation of 3alpha-hydroxy group
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
gastrointestinal bacteria
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
2 * 30000, SDS-PAGE
53000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 30000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE