Information on EC 1.1.1.217 - benzyl-2-methyl-hydroxybutyrate dehydrogenase

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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

EC NUMBER
COMMENTARY hide
1.1.1.217
-
RECOMMENDED NAME
GeneOntology No.
benzyl-2-methyl-hydroxybutyrate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + NADP+ = benzyl 2-methyl-3-oxobutanoate + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
benzyl-(2R,3S)-2-methyl-3-hydroxybutanoate:NADP+ 3-oxidoreductase
Also acts on benzyl (2S,3S)-2-methyl-3-hydroxybutanoate; otherwise highly specific.
CAS REGISTRY NUMBER
COMMENTARY hide
99332-62-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
show the reaction diagram
-
-
ratio syn-(2) and anti-(3) conformation 8:1 for purified enzyme
r
additional information
?
-
-
no reduction of aldehydes or alcohols
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
show the reaction diagram
-
-
-
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ag+
-
strong inhibition
Cu2+
-
strong inhibition
Hg2+
-
strong inhibition
Ni2+
-
strong inhibition
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.2
benzyl (2R,3S)-2-methyl-3-hydroxy-butanoate
-
-
0.42
Benzyl 2-methyl-3-oxobutyrate
-
-
4.2
benzyl(2S,3S)-2-methyl-3-hydroxybutanoate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0063
-
crude extract
0.9
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
-
less than 10% activity at pH 4 and 9
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
-
SDS-PAGE
32000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
purified enzyme at pH 6-8, rapid loss of activity below pH 6 and above pH 9
286280
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
rapid loss of activity above 40°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE