Information on EC 1.1.1.216 - farnesol dehydrogenase (NADP+)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.216
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RECOMMENDED NAME
GeneOntology No.
farnesol dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E,6E)-farnesol + NADP+ = (2E,6E)-farnesal + NADPH + H+
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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farnesylcysteine salvage pathway
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Insect hormone biosynthesis
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juvenile hormone III biosynthesis I
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juvenile hormone III biosynthesis II
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Sesquiterpenoid and triterpenoid biosynthesis
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Terpenoid backbone biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesol:NADP+ 1-oxidoreductase
Also acts, more slowly, on (2Z,6E)-farnesol, geraniol, citronerol and nerol.
CAS REGISTRY NUMBER
COMMENTARY hide
90804-55-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
NADP+-dependent farnesol dehydrogenase 1
UniProt
Manually annotated by BRENDA team
sweet potato, enzyme is formed in response to infection by the black rot fungus Ceratocystis fimbriata
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-
Manually annotated by BRENDA team
tobacco hornworm
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesol + NADP+
(2E,6E)-farnesal + NADPH + H+
show the reaction diagram
enzyme shows highest affinity for farnesol
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-
i
(2Z,6Z)-farnesol + NADP+
(2Z,6Z)-farnesal + NADPH + H+
show the reaction diagram
enzyme shows highest affinity for farnesol
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-
r
(E)-geraniol + NADP+
(E)-geranial + NADPH + H+
show the reaction diagram
-
-
-
?
(Z)-nerol + NADP+
(Z)-neral + NADPH + H+
show the reaction diagram
-
-
-
?
2-decanol + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
?
cis,trans-farnesol + NADP+
cis,trans-farnesal + NADPH
show the reaction diagram
-
-
-
?
citronellol + NADP+
citronellal + NADPH + H+
show the reaction diagram
-
-
-
?
citronerol + NADP+
?
show the reaction diagram
-
-
-
-
?
decanol + NADP+
decanal + NADPH
show the reaction diagram
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-
-
-
?
farnesol + NADP+
farnesal + NADPH
show the reaction diagram
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-
-
?
geraniol + NADP+
?
show the reaction diagram
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-
-
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?
nerol + NADP+
?
show the reaction diagram
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-
-
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?
octanol + NADP+
octanal + NADPH + H+
show the reaction diagram
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-
-
?
trans,trans-farnesol + NADP+
trans,trans-farnesal + NADPH
show the reaction diagram
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preferred substrate
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r
additional information
?
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no activity is detected for butanol, ethanol, or glycerol, or for farnesal or farnesoic acid
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
farnesol + NADP+
farnesal + NADPH
show the reaction diagram
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-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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weak irreversible inhibitor
glucose oxidase
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Monoiodoacetamide
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N-ethylmaleimide
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Sodium dithionite
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additional information
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inhibitory effect of thiol reagents indicates that the SH-group of the enzyme participates in its activity
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
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slightly enhances activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.091
(2E,6E)-farnesol
pH 10.0, 30C
0.097
(2Z,6Z)-farnesol
pH 10.0, 30C
0.208
(E)-geraniol
pH 10.0, 30C
0.109
(Z)-nerol
pH 10.0, 30C
0.184
2-decanol
pH 10.0, 30C
0.133
citronellol
pH 10.0, 30C
0.001
farnesol
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0.729
geraniol
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0.195
Octanol
pH 10.0, 30C
0.0156
trans,trans-farnesol
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
substrate (2Z,6Z)-farnesol, pH 10.0, 30C
9.7
substrate (2E,6E)-farnesol, pH 10.0, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
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7 - 7.5
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reduction of trans,trans-farnesal
9.5
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oxidation of trans,trans-farnesol
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
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reduction of trans,trans-farnesal: 60% of maximal activity at pH 6.5 and at pH 8
7 - 10.5
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oxidation of trans,trans-farnesol: 10% of maximal activity at pH 7.0, 30% of maximal activity at pH 10.2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
and midgut, of adult female, highest levels of mRNA
Manually annotated by BRENDA team
no farnesol dehydrogenase activity in newly emerged mosquitoes but significant activity 24 h later. SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis
Manually annotated by BRENDA team
and Malpighian tubules, considerable levles of mRNA
Manually annotated by BRENDA team
abdominal and thoracic ganglia, high level of mRNA
Manually annotated by BRENDA team
and fat body, considerable levles of mRNA
Manually annotated by BRENDA team
and brain, of adult female, highest levels of mRNA
Manually annotated by BRENDA team
of adult male, substantial level of mRNA
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
2 * 29000, calculated
47000
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2 * 47000, SDS-PAGE
60000
gel filtration
90000
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gel filtation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis