Information on EC 1.1.1.212 - 3-oxoacyl-[acyl-carrier-protein] reductase (NADH)

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The expected taxonomic range for this enzyme is: Persea americana

EC NUMBER
COMMENTARY hide
1.1.1.212
-
RECOMMENDED NAME
GeneOntology No.
3-oxoacyl-[acyl-carrier-protein] reductase (NADH)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NAD+ = a 3-oxoacyl-[acyl-carrier protein] + NADH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(3R)-3-hydroxyacyl-[acyl-carrier protein]:NAD+ oxidoreductase
Forms part of the fatty acid synthase system in plants. Can be separated from EC 1.1.1.100, 3-oxoacyl-[acyl-carrier-protein] reductase.
CAS REGISTRY NUMBER
COMMENTARY hide
82047-86-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
avocado
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetoacetyl-CoA + NADH
3-hydroxybutanoyl-CoA + NAD+
show the reaction diagram
-
-
-
-
?
acetoacetyl-N-acetylcysteamine + NADH
3-hydroxybutanoyl-N-acetylcysteamine + NAD+
show the reaction diagram
-
very poor substrate
-
-
?
acetoacetyl-[acyl-carrier protein] + NADH
3-hydroxybutanoyl-[acyl-carrier protein] + NAD+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetoacetyl-[acyl-carrier protein] + NADH
3-hydroxybutanoyl-[acyl-carrier protein] + NAD+
show the reaction diagram
-
component of fatty acid synthetase system
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.2
acetoacetyl N-acetyl-cysteamide
-
-
0.155
acetoacetyl-CoA
-
-
0.00965
acetoacetyl-[acyl-carrier protein]
-
-
0.00273
NADH
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0098
-
12000 x g precipitate of homogenate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
168000
-
sucrose density gradient centrifugation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50 mM phosphate buffer, pH 7.0, 10% glycerol, stable for two weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE