Information on EC 1.1.1.210 - 3beta(or 20alpha)-hydroxysteroid dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.210
-
RECOMMENDED NAME
GeneOntology No.
3beta(or 20alpha)-hydroxysteroid dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5alpha-androstan-3beta,17beta-diol + NADP+ = 17beta-hydroxy-5alpha-androstan-3-one + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3beta(or 20alpha)-hydroxysteroid:NADP+ oxidoreductase
Also acts on 20alpha-hydroxysteroids.
CAS REGISTRY NUMBER
COMMENTARY hide
82869-26-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
female Wistar rats
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
17beta-hydoxy-5alpha-androstan-3-one + NADPH
5alpha-androstan-3beta,17beta-diol + NADP+
show the reaction diagram
17beta-hydroxy-5alpha-androstan-3-one + NADPH
5alpha-androstan-3beta,17beta-diol + NADP+
show the reaction diagram
progesterone + NADPH
20alpha-hydroxy-4-pregnen-3-one + NADP+
show the reaction diagram
additional information
?
-
-
enzyme is involved in progesterone biosynthesis
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
17beta-hydroxy-5alpha-androstan-3-one + NADPH
5alpha-androstan-3beta,17beta-diol + NADP+
show the reaction diagram
progesterone + NADPH
20alpha-hydroxy-4-pregnen-3-one + NADP+
show the reaction diagram
additional information
?
-
-
enzyme is involved in progesterone biosynthesis
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16alpha-(Bromoacetoxy)progesterone
-
competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation, 2-mercaptoethanol protects
17beta-hydroxy-5alpha-androstan-3-one
19-Nortestosterone 17-bromoacetate
-
competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation
5alpha-dihydrotestosterone 17-bromoacetate
-
competitive inhibition of 3beta- and 20alpha-reductase activity, time dependent and simultaneous, complete inactivation
progesterone
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.074
17beta-hydroxy-5alpha-androstan-3-one
-
-
0.0025 - 0.0094
NADPH
0.031
progesterone
-
-
additional information
additional information
-
kinetic studies
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0071
-
20alpha-reductase activity
0.0096
-
20alpha-reductase activity
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6
-
-
8
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
enzyme activity during stages of pseudo-pregnancy, 20alpha-hydroxysteroid dehydrogenase activity increases from day 1 to day 12, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
20alpha-hydroxysteroid dehydrogenase activity
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
-
gel filtration
36000
-
SDS-PAGE
55000
-
SDS-PAGE, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
lyophilization, complete loss of activity
-
stable to bromoacetic acid
-
stable to ethyl bromoacetate
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, purified enzyme, indefinitely stable
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE