Information on EC 1.1.1.20 - glucuronolactone reductase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY hide
1.1.1.20
-
RECOMMENDED NAME
GeneOntology No.
glucuronolactone reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-gulono-1,4-lactone + NADP+ = D-glucurono-3,6-lactone + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ascorbate and aldarate metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
L-gulono-1,4-lactone:NADP+ 1-oxidoreductase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9028-30-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-galacturonic acid + NADPH
?
show the reaction diagram
-
-
-
-
?
D-glucuronic acid + NADPH
L-gulonic acid + NADP+
show the reaction diagram
-
-
-
-
?
D-glucurono-3,6-lactone + NADPH
D-gulono-1,4-lactone + NADP+
show the reaction diagram
-
oxidation activity is very low
-
-
r
D-iduronic acid + NADPH
?
show the reaction diagram
-
-
-
-
?
erythrose + NADPH
glycerol + NADP+
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NADPH
?
show the reaction diagram
-
-
-
-
?
L-gulono-1,4-lactone + NADP+
D-glucurono-3,6-lactone + NADPH
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-gulono-1,4-lactone + NADP+
D-glucurono-3,6-lactone + NADPH
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
NADPH is 145-times as effective as NADH
NADPH
-
NADPH is 145-times as effective as NADH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Barbital
-
-
Barbiturates
-
-
-
dithiothreitol
-
-
iodoacetic acid
-
-
Phenobarbital
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4
D-galacturonic acid
-
-
6
D-glucuronic acid
-
L-iduronic acid
9
D-glucuronolactone
-
-
0.045
NADPH
-
with D-glucuronic acid
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
13450 U/min * mg, 1 unit is defined as change of absorbance of 0.01/min at 340 nm
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.5
-
DL-glyceraldehyde
6 - 7
-
D-glucuronic acid
9.5
-
broad, oxidation of glycerol and L-gulonolactone
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 40000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
180 min, 50% loss of activity; 30 min, 20% loss of activity; 60 min, 30% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing: 3 times causes 25% decrease of activity, 5 times causes 40% decrease of activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, 10 days, 30% loss of activity
-
0°C, 2 months, less than 10% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE