Information on EC 1.1.1.198 - (+)-borneol dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.198
-
RECOMMENDED NAME
GeneOntology No.
(+)-borneol dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(+)-borneol + NAD+ = (+)-camphor + NADH + H+
show the reaction diagram
NADP+ can also act, but more slowly
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(+)-camphor biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
Monoterpenoid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
(+)-borneol:NAD+ oxidoreductase
NADP+ can also act, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
67185-75-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-borneol + NAD+
(+)-camphor + NADH
show the reaction diagram
(+)-borneol + NAD+
(+)-camphor + NADH + H+
show the reaction diagram
(+)-dihydrocarveol + NAD+
(-)-dihydrocarvone + NADH
show the reaction diagram
-
poor substrate
-
?
(+)-endo-fenchol + NAD+
1,3,3-trimethyl-bicyclo[2.2.1]heptan-2-one + NADH
show the reaction diagram
-
26% of the activity compared to (+)-borneol
-
?
(+)-isoborneol + NAD+
(+)-isocamphor + NADH
show the reaction diagram
-
nearly the same activity compared to (+)-borneol
-
?
(-)-artemisia alcohol + NAD+
artemisia ketone + NADH + H+
show the reaction diagram
-
-
-
?
(-)-borneol + NAD+
(+)-camphor + NADH + H+
show the reaction diagram
-
-
-
?
(-)-borneol + NAD+
(-)-camphor + NADH
show the reaction diagram
-
36% of the activity compared to (+)-borneol
-
?
(-)-cis-carveol + NAD+
(-)-carvone + NADH + H+
show the reaction diagram
-
-
-
?
(-)-endo-fenchol + NAD+
1,3,3-trimethyl-bicyclo[2.2.1]heptan-2-one + NADH
show the reaction diagram
-
poor substrate
-
?
(-)-isoborneol + NAD+
(-)-isocamphor + NADH
show the reaction diagram
-
30% of the activity compared to (+)-borneol
-
?
(-)-neothujol + NAD+
(-)-neothujone + NADH
show the reaction diagram
-
65% of the activity compared to (+)-borneol
-
?
(-)-thujol + NAD+
(-)-thujone + NADH
show the reaction diagram
-
2fold higher activity than for (+)-borneol
-
?
(-)-trans-carveol + NAD+
(-)-carvone + NADH + H+
show the reaction diagram
-
-
-
?
(-)-trans-pinocarveol + NAD+
(-)-pinocarvone + NADH + H+
show the reaction diagram
-
-
-
?
(1R)-menthol + NAD+
(1R)-trans-p-menthan-3-one + NADH
show the reaction diagram
-
poor substrate
-
?
geraniol + NAD+
geranial + NADH
show the reaction diagram
-
poor substrate
-
?
nerol + NAD+
neryl aldehyde + NADH
show the reaction diagram
-
poor substrate
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(+)-borneol + NAD+
(+)-camphor + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
additional information
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(+)-Camphor
-
27% inhibition at 0.5 mM
(-)-Thujol
-
competitive inhibition of (+)-borneol oxidation
8-hydroxyquinoline
-
57% inhibition at 1 mM
Borate
-
inhibition of borneol oxidation
Cu2+
-
85% inhibition at 2 mM
ethylenediaminetetraacetate
-
slight inhibition at 1 mM
Hg2+
-
nearly complete inhibition at 2 mM
iodoacetamide
-
41% inhibition at 1 mM
N-ethylmaleimide
-
53% inhibition at 1 mM
NaCN
-
slight inhibition at 1 mM
NADH
-
46% inhibition at 1 mM
NaN3
-
slight inhibition at 1 mM
o-phenanthroline
-
slight inhibition at 1 mM
p-hydroxymercuribenzoate
-
50% inhibition at 0.05 mM
Pb2+
-
26% inhibition at 2 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03 - 0.053
(+)-borneol
0.086
(-)-artemisia alcohol
pH 9.5, 30C
0.027
(-)-cis-carveol
pH 9.5, 30C
0.041
(-)-Thujol
-
-
0.07
NAD+
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0004
(+)-borneol
Lavandula x intermedia
K4N0V2
pH 8.0, 32C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.17
substrate (-)-borneol, pH 9.5, 30C
5.33
substrate (+)-borneol, pH 9.5, 30C
10.33
substrate (-)-trans-pinocarveol, pH 9.5, 30C
13.5
substrate (-)-trans-carveol, pH 9.5, 30C
35.7
substrate (-)-artemisia alcohol, pH 9.5, 30C
147.4
substrate (-)-cis-carveol, pH 9.5, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
transcripts are specifically expressed in glandular trichomes of mature flowers
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27500
x * 27500, calculated
91000
-
gel filtration
92000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 27500, calculated
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
most stable around
286163
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0C, 0.1 M sodium phosphate, pH 8.0, 15% glycerol, 20 mM 2-mercaptoethanol, 3 days, 25% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
near homogeneity, chromatography techniques, affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli