Information on EC 1.1.1.19 - glucuronate reductase

Word Map on EC 1.1.1.19
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.19
-
RECOMMENDED NAME
GeneOntology No.
glucuronate reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-gulonate + NADP+ = D-glucuronate + NADPH + H+
show the reaction diagram
also reduces D-galacturonate; may be identical with EC 1.1.1.2 (alcohol dehydrogenase (NADP)) e.g. in
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-glucuronate degradation I
-
-
L-ascorbate biosynthesis IV
-
-
ascorbate metabolism
-
-
Pentose and glucuronate interconversions
-
-
Ascorbate and aldarate metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
L-gulonate:NADP+ 6-oxidoreductase
Also reduces D-galacturonate. May be identical with EC 1.1.1.2 [alcohol dehydrogenase (NADP+)].
CAS REGISTRY NUMBER
COMMENTARY hide
9028-29-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrobenzaldehyde + NADPH
4-nitrobenzyl alcohol + NADP+
show the reaction diagram
D-aldose + NADPH
L-alditol + NADP+
show the reaction diagram
D-galacturonate + NADPH
?
show the reaction diagram
-
-
-
-
r
D-glucuronate + NADPH
L-gulonate + NADP+
show the reaction diagram
D-glucuronate + NADPH + H+
? + NADP+
show the reaction diagram
-
ALR1
-
-
?
D-glucuronate + NADPH + H+
L-gulonate + NADP+
show the reaction diagram
D-glucuronolactone + NADPH
L-gulonolactone + NADP+
show the reaction diagram
D-glyceraldehyde + NADPH + H+
glycerol + NADP+
show the reaction diagram
gamma-hydroxybutyrate + NADP+
succinic semialdehyde + NADPH
show the reaction diagram
L-gulonate + NADP+
D-glucuronate + NADPH
show the reaction diagram
pyridine-3-aldehyde + NADPH
3-hydroxypyridine + NADP+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glucuronate + NADPH
L-gulonate + NADP+
show the reaction diagram
D-glucuronate + NADPH + H+
? + NADP+
show the reaction diagram
-
ALR1
-
-
?
D-glucuronolactone + NADPH
L-gulonolactone + NADP+
show the reaction diagram
gamma-hydroxybutyrate + NADP+
succinic semialdehyde + NADPH
show the reaction diagram
-
preverably in a coupled reaction in vivo with reduction of D-glucuronate
-
-
?
L-gulonate + NADP+
D-glucuronate + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3-Butanedione
-
-
2-mercaptoethanol
acetoacetate
Al 1576
AL-1567
alpha-ketoglutarate
Amaranth
-
-
ATP-ribose
-
competitive versus NADPH
Barbiturates
-
-
-
branched alpha-ketoacids
-
diethyldicarbonate
-
-
Diphenylhydantoin
-
-
dithiothreitol
DL-beta-hydroxybutyrate
haematin
-
-
hydroxylamine
iodoacetic acid
menadione
-
competitive versus D-glucuronate
N-ethylmaleimide
p-chloromercuribenzoate
Phenobarbital
phenolsulfophthalein
-
-
-
phenylacetate
Phenylglyoxal
-
-
Ponalrestat
pyridoxal 5'-phosphate
Salicylates
-
Sorbinil
Statil
-
-
Sulfobromophthalein
-
-
Tolrestat
Valproate
-
poor
Zn2+
-
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
500
2-deoxyglucose
-
-
0.769
2-nitrobenzaldehyde
-
-
0.526
3-Nitrobenzaldehyde
-
-
0.133 - 0.35
4-Nitrobenzaldehyde
1.05
benzaldehyde
-
-
139.9
D-arabinose
-
-
333
D-galactose
0.0543 - 9.1
D-glucuronate
0.69
D-glucuronolactone
-
-
4.8
D-glyceraldehyde
-
-
333
D-ribose
1.671
DL-glyceraldehyde
-
-
0.00045 - 0.0025
gamma-hydroxybutyrate
2.1 - 7.6
L-gulonate
0.0014 - 0.02
NADP+
0.009 - 0.036
NADPH
2.6
Pyridine-3-aldehyde
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
Homo sapiens
-
0.119 per mM * s, L-gulonate, steady state kinetic; 10.82 per mM * s, D-glucuronate, steady state kinetic; 1124 per mM * s, NADPH, steady state kinetic
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0025
1.339
-
highly purified enzyme, substrate NADPH
5.45
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
-
DL-glyceraldehyde
6.2 - 6.6
-
D-glucuronate
7
-
shifted pH-optimum for the coupled reaction of oxidation of gamma-hydroxybutyrate and reduction of D-glucuronate in vitro
8
-
assay at, ALR1
8.5
-
substrate L-gulonate or D-glucuronate
8.6 - 9.3
-
-
9
-
substrate gamma-hydroxybutyrate, at pH 7.0 activity is reduced by 50%
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at, ALR1
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
low activity
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
-
SDS-PAGE, gel filtration
35000
-
gel filtration
36000
-
gel filtration
39000
-
gel filtration
60000 - 70000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10.5
-
irreversible loss of activity below pH 5.0 and above pH 11
286149
7
-
most stable
286141
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
15 min, 50% loss of activity, ammonium sulfate fraction
50
-
15 min, complete inactivation, ammonium sulfate fraction
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
EDTA 1 mM protects against heat denaturation at 40°C
-
lyophilization destroys activity
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acetone
-
inactivates
Ethanol
-
inactivates
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-5°C, 2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
no exact differentiation between organisms in the reference