Information on EC 1.1.1.175 - D-xylose 1-dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.175
-
RECOMMENDED NAME
GeneOntology No.
D-xylose 1-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-xylose + NAD+ = D-xylonolactone + NADH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
d-xylose degradation
-
-
Pentose and glucuronate interconversions
-
-
SYSTEMATIC NAME
IUBMB Comments
D-xylose:NAD+ 1-oxidoreductase
-
CAS REGISTRY NUMBER
COMMENTARY hide
62931-20-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 588
-
-
Manually annotated by BRENDA team
strain 588
-
-
Manually annotated by BRENDA team
strain NA1000, gene xylB, the enzyme is encoded in the xylose-inducible xylXABCD operon
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain MSU-1
-
-
Manually annotated by BRENDA team
porcine
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-galactose + NAD+
? + NADH
show the reaction diagram
-
low activity
-
?
D-glucose + NAD+
? + NADH
show the reaction diagram
-
-
-
?
D-lyxose + NAD+
? + NADH
show the reaction diagram
-
low activity
-
?
D-xylose + NAD+
?
show the reaction diagram
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
show the reaction diagram
D-xylose + NAD+
D-xylonolactone + NADH + H+
show the reaction diagram
L-arabinose + NAD+
? + NADH
show the reaction diagram
-
-
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH + H+
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-galactose + NAD+
? + NADH
show the reaction diagram
-
low activity
-
?
D-glucose + NAD+
? + NADH
show the reaction diagram
-
-
-
?
D-lyxose + NAD+
? + NADH
show the reaction diagram
-
low activity
-
?
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
show the reaction diagram
D-xylose + NAD+
D-xylonolactone + NADH + H+
show the reaction diagram
L-arabinose + NAD+
? + NADH
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
30
D-galactose
-
strain 89B
0.08 - 17.4
D-xylose
5.9 - 180
L-arabinose
0.2 - 0.46
NAD+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
22.7 - 30.3
D-xylose
22.3 - 29.2
L-arabinose
25.8 - 35.7
NAD+
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
66.7 - 280
D-xylose
115
0.12 - 0.6
L-arabinose
206
66.7 - 115
NAD+
7
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30.9
-
strain 89B
143
-
strain 90A
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9 - 10
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 35
-
-
40 - 55
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
62000
-
gel filtration
67000
-
gel filtration
89000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3 - 9
-
stable within, strain 89B
286115
6 - 7.5
-
stable within, strain 90A
286115
6.5 - 7.8
-
stable within, no enzymatic activity
286116
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
stable below
32
-
half-life: 2 min
50
-
complete inactivation
65
half-life: 56.8 h, in the presence of 3 M NaCl, stability decreases significantly with lower salt concentrations
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
thermolabile, stabilizing: substrates or high ionic strength
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, stable for several months
frozen, addition of DTT, several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
NAD+-affinity chromatography
-
partial
-
to homogeneity, chromatography techniques
-
to homogeneity, chromatography techniques, isoelectric focusing
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
expression of gene xylB in Saccharomyces cerevisiae results in production of 17 g D-xylonate/l at 0.23g/l/h from 23 g D-xylose/l. D-Xylonate accumulates intracellularly to 70 mg/g, xylitol to 18 mg/g. Cells expressing D-xylonolactone lactonase xylC from Caulobacter crescentus with xylB initially produce more extracellular D-xylonate than cells lacking xylC at both pH5.5 and pH3, and sustain higher production at pH3. Cell vitality and viability decreases during D-xylonate production at pH 3.0