Information on EC 1.1.1.156 - glycerol 2-dehydrogenase (NADP+)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.156
-
RECOMMENDED NAME
GeneOntology No.
glycerol 2-dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
glycerol + NADP+ = glycerone + NADPH + H+
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
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reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycerolipid metabolism
-
-
Metabolic pathways
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-
SYSTEMATIC NAME
IUBMB Comments
glycerol:NADP+ 2-oxidoreductase (glycerone-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
39342-20-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
strain IFO 3255
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-
Manually annotated by BRENDA team
strain IFO 3255
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-
Manually annotated by BRENDA team
low levels of activity
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-
Manually annotated by BRENDA team
Schizosaccharomyces slooffiae
low levels of activity
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-
Manually annotated by BRENDA team
also known as hypocrea jecorina
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
under osmotic stress, G3pdh and Dhar play important roles in glycerol accumulation or degradation in Dunaliella for osmotic adjustment to respect to various salinities
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2,3-butanetriol + NADP+
1,3-dihydroxybutan-2-one + NADPH
show the reaction diagram
-
18% of the activity compared to glycerol
-
?
1,2-butanediol + NADP+
1-hydroxybutan-2-one + NADPH
show the reaction diagram
-
19% of the activity compared to glycerol
-
?
1,2-ethanediol + NADP+
hydroxyacetaldehyde + NADPH
show the reaction diagram
-
0.3% of the activity compared to glycerol
-
r
1,2-propanediol + NADP+
hydroxyacetone + NADPH
show the reaction diagram
2,3-butanediol + NADP+
3-hydroxybutan-2-one + NADPH
show the reaction diagram
dihydroxyacetone + NADH + H+
glycerol + NAD+
show the reaction diagram
dihydroxyacetone + NADPH + H+
glycerol + NADP
show the reaction diagram
-
-
-
ir
dihydroxyacetone + NADPH + H+
glycerol + NADP+
show the reaction diagram
dihydroxyacetonephosphate + NADPH
glycerol 3-phosphate + NADP+
show the reaction diagram
glycerol + NADP+
dihydroxyacetone + NADPH
show the reaction diagram
iso-erythritol + NADP+
? + NADPH
show the reaction diagram
-
0.9% of the activity compared to glycerol
-
r
L-arabitol + NADP+
? + NADPH
show the reaction diagram
-
11% of the activity compared to glycerol
-
?
L-glyceraldehyde + NADPH
L-glycerol + NADP+
show the reaction diagram
L-ribulose + NADPH
? + NADP+
show the reaction diagram
-
very low activity
-
?
meso-erythritol + NADP+
? + NADPH
show the reaction diagram
methylglyoxal + NADPH
2-hydroxypropanal + NADP+
show the reaction diagram
-
very low activity
-
?
propan-2-ol + NADP+
acetone + NADPH
show the reaction diagram
-
very low activity
-
?
pyruvaldehyde + NADPH
lactaldehyde + NADP+
show the reaction diagram
-
very low activity
-
?
sedoheptulose + NADPH
? + NADP+
show the reaction diagram
-
very low activity
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glycerol + NADP+
dihydroxyacetone + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
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forward reaction highest activity at 4.5 M, reverse reaction at 3.5 M
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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80% inhibition at 1 mM
8-hydroxyquinoline
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complete inhibition at 10 mM
dithiothreitol
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25% inhibition at 10 mM
KCl
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inhibition above 100 mM
L-cysteine
-
40% inhibition at 10 mM
Mg2+
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40% inhibition at 5 mM
Mn2+
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25% inhibition at 5 mM
p-chloromercuribenzoate
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strong inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0083 - 3.9
dihydroxyacetone
51 - 2437
glycerol
0.015 - 0.167
NADP+
0.004 - 0.34
NADPH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.06
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crude extract, at 25C
13.71
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after 228.5fold purification, at 25C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 6.5
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dihydroxyacetone reduction
6
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dihydroxyacetone reduction
7.4
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assay reverse reaction
7.5
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dihydroxyacetone reduction
9
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glycerol oxidation
9.2
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assay forward reaction
9.4
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glycerol oxidation
9.5
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glycerol oxidation
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
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SDS-PAGE
34000 - 39000
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gel filtration, SDS-PAGE
57000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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1 * 30000 + 1 * 25000, SDS-PAGE
monomer
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1 * 39000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
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little loss of activity
70
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complete inactivation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-cellulose column chromatography, hydroxyapatite column chromatography, BD Sepharose column chromatography, and DEAE-Sephadex A-50 gel filtration
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to homogeneity, 1step chromatography
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to homogeneity, chromatography techniques
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae