Information on EC 1.1.1.148 - estradiol 17alpha-dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.148
-
RECOMMENDED NAME
GeneOntology No.
estradiol 17alpha-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
estradiol-17alpha + NAD(P)+ = estrone + NAD(P)H + H+
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
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redox reaction
-
-
-
-
reduction
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Steroid hormone biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
17alpha-hydroxysteroid:NAD(P)+ 17-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
9044-91-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
inducible by growth on androstenedione and cholic acid, not deoxycholic acid; VPI 12708
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-
Manually annotated by BRENDA team
VPI 12708
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-
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
17alpha-estradiol + NAD(P)+
estrone + NAD(P)H
show the reaction diagram
17alpha-hydroxy-4-androsten-3-one + NADP+
4-androsten-3,17-dione + NADPH
show the reaction diagram
17beta-hydroxy-4-androsten-3-one + NADP+
4-androsten-3,17-dione + NADPH
show the reaction diagram
-
testosterone
-
-
?
5alpha-androstan-3beta,17alpha-diol + NADP+
3beta-hydroxy-5alpha-androstan-17-one + NADPH
show the reaction diagram
-
-
-
-
?
5alpha-dihydrotestosterone + NADPH
5alpha-androstane-3alpha,17alpha-diol + NADP+
show the reaction diagram
-
-
-
?
5alpha-pregnane-3,20-dione + NADPH + H+
3alpha,5alpha-3-hydroxypregnan-20-one + NADP+
show the reaction diagram
-
-
-
?
androstanedione + NADPH
5alpha-androstane-3-one-17alpha-ol + NADP+
show the reaction diagram
-
-
-
?
androstenedione + NADPH + H+
epitestosterone + NADP+
show the reaction diagram
-
-
-
?
androsterone + NADPH
5alpha-androstane-3alpha,17alpha-diol + NADP+
show the reaction diagram
-
-
-
?
dehydroepiandrosterone + NADPH
5-androstene-3alpha,17alpha-diol + NADP+
show the reaction diagram
-
-
-
?
estradiol-17alpha 3-galacturonide + NADP+
estrone 3-galacturonide + NADPH
show the reaction diagram
-
-
-
-
?
estradiol-17alpha 3-glucoside + NADP+
estrone 3-glucoside + NADPH
show the reaction diagram
-
-
-
-
?
estradiol-17alpha 3-glucuronide + NADP+
estrone 3-glucuronide + NADPH
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17beta-(1-Oxoprop-2-ynyl)androst-4-ene-3-one
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Epitestosterone
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substrate inhibition for all isoforms at concentration above 0.003 mM, not observed with other substrates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0017 - 0.025
17alpha-estradiol
0.001 - 0.0051
17alpha-estradiol 3-glucuronide
-
for six isoforms
0.031
4-androsten-3,17-dione
-
-
0.0002 - 0.07
Epitestosterone
0.002 - 0.011
NADP+
0.005
NADPH
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003 - 0.009
-
isoform IA, IB, IID, III, 17alpha-estradiol 3-glucuronide
0.007 - 0.015
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six isoforms, 17alpha-estradiol
0.041 - 0.2
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six isoforms, epitestosterone
0.047
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isoform IIC, 17alpha-estradiol 3-glucuronide
0.18
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isoform IC, 17alpha-estradiol 3-glucuronide
1.8
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oxidation of 17alpha-hydroxy-4-androsten-3-one
3.25
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reduction of 4-androsten-3,17-dione
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7
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reduction of 4-androsten-3,17-dione
6.5
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oxidation of 17alpha-hydroxy-4-androsten-3-one
7.3
assay at
9 - 9.5
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 10
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-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
high expression level
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
-
x * 33000, SDS-PAGE
39600
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x * 39600, SDS-PAGE, all 8 forms
42000
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4 * 42000, SDS-PAGE
52500
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gel filtration
160000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
sensitive to molecular oxygen
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% v/v glycerol
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4°C, 5 mM phosphate buffer, pH 7.2, 20% v/v glycerol, at least 3 days
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4°C, phosphate buffer, pH 7.0, 1 mM dithiothreitol, at least 1 month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 forms
-
8 forms, six of them purified to homogenity: IA, IB, IC, IIC, IID, III
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partial
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recombinant GST-fusion enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA cloning, subcloning in Escherichia coli, expression as GST-fusion protein in Escherichia coli strain BL21(DE3), stable expression in HEK-293 cells