Information on EC 1.1.1.124 - fructose 5-dehydrogenase (NADP+)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.124
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RECOMMENDED NAME
GeneOntology No.
fructose 5-dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-fructose + NADP+ = 5-dehydro-D-fructose + NADPH + H+
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
-
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reduction
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-
-
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SYSTEMATIC NAME
IUBMB Comments
D-fructose:NADP+ 5-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-53-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-keto-D-fructose 1-phosphate + NADPH + H+
D-fructose 1-phosphate + NADP+
show the reaction diagram
D-arabinose + NADP+
? + NADPH
show the reaction diagram
-
5fold lower activity than for D-fructose
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?
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-fructose + NADP+
5-dehydro-D-fructose + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
substitutes for NADPH
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-fructose
NADP+
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competitive inhibition of D-fructose synthesis, non-competitive inhibition of 5-keto-fructose synthesis
NADPH
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competitive inhibition of D-fructose oxidation
sulfhydryl reagents
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.5 - 12.7
5-keto-D-fructose
4.2
5-Keto-D-fructose 1-phosphate
-
-
140
D-arabinose
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9.1 - 70
D-fructose
1.97
NAD+
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-
0.32
NADH
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0.053 - 1.3
NADP+
0.002 - 0.071
NADPH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 5
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D-fructose oxidation
620
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5-keto-D-fructose reduction
2450
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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reduction of 5-keto-D-fructose
7.4
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NADPH oxidation in Tris-HCl buffer
8.5 - 10
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D-fructose oxidation
9.5
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oxidation of D-fructose
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
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4 * 25000, SDS-PAGE
40000
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1 * 40000, SDS-PAGE
50000
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gel filtration, sedimentation equilibrium measurement
100000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 40000, SDS-PAGE
tetramer
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4 * 25000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 8
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stable between
285815
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 30 mM Tris-HCl, pH 7.8, 3 months
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4°C, stable for 3-4 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
high purity to crystalline form, chromatography techniques
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to homogeneity, chromatography techniques
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