Information on EC 1.1.1.116 - D-arabinose 1-dehydrogenase (NAD+)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.116
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RECOMMENDED NAME
GeneOntology No.
D-arabinose 1-dehydrogenase (NAD+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-arabinose + NAD+ = D-arabinono-1,4-lactone + NADH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
dehydro-D-arabinono-1,4-lactone biosynthesis
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degradation of pentoses
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SYSTEMATIC NAME
IUBMB Comments
D-arabinose:NAD+ 1-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-47-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ARA2; gene YMR041c or ARA2, isozyme Ara2p
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-ribose + NAD+
2-deoxy-D-ribono-1,4-lactone + NADH
show the reaction diagram
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
show the reaction diagram
D-arabinose + NADP+
D-arabinono-1,4-lactone + NADPH
show the reaction diagram
-
-
-
-
?
D-glucose + NAD+
D-glucono-1,5-lactone + NADH + H+
show the reaction diagram
-
-
-
-
?
D-lyxose + NAD+
D-lyxono-1,5-lactone + NADH
show the reaction diagram
-
-
-
-
?
D-ribose + NAD+
D-ribono-1,4-lactone + NADH
show the reaction diagram
D-xylose + NAD+
D-xylono-1,4-lactone + NADH
show the reaction diagram
digitoxose + NAD+
?
show the reaction diagram
-
-
-
-
?
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH
show the reaction diagram
L-fucose + NAD+
?
show the reaction diagram
L-fucose + NAD+
L-6-deoxyascorbate + NADH
show the reaction diagram
-
-
-
-
?
L-fucose + NAD+
L-fuconolactone + NADH
show the reaction diagram
L-galactose + NAD+
?
show the reaction diagram
L-galactose + NAD+
L-ascorbate + NADH
show the reaction diagram
-
-
-
-
?
L-galactose + NADP+
L-galactono-1,5-lactone + NADPH
show the reaction diagram
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-
-
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?
L-xylose + NAD+
?
show the reaction diagram
-
-
-
?
L-xylose + NADP+
L-xylono-1,4-lactone + NADPH
show the reaction diagram
-
-
-
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
show the reaction diagram
L-fucose + NAD+
L-6-deoxyascorbate + NADH
show the reaction diagram
-
-
-
-
?
L-galactose + NAD+
L-ascorbate + NADH
show the reaction diagram
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-
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-arabinose
D-glucose
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excess concentration
HgCl2
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iodoacetamide
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L-fucose
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at high concentrations
L-Xylose
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excess concentration
N-ethylmaleimide
NADH
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non competitive inhibition at non-saturating NAD+-level
p-chloromercuribenzoate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
24
2-deoxy-D-ribose
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0.5 - 29.2
D-arabinose
670
D-ribose
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49
digitoxose
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0.18 - 28.2
L-fucose
91.3
L-galactose
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37.1
L-Xylose
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0.017 - 4.1
NAD+
0.048
NADP+
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00071
NAD+-dependent Ara2p reaction in presence of 200 mM D-arabinose, native cell extract
0.432
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D-arabinose
0.605
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cosubstrate L-fucose
1.8
purified recombinant His-tagged ARA2p
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.6
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Tris/HCl buffer
10
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glycine/NaOH buffer
10.6
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
20% of maximal activity at pH 6.5 and pH 9.0, pH profile
8.5 - 10.5
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about 75% of maximal activity at pH 8.5 and 10.5
9.4 - 11.8
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about 60% of maximal activity at pH 9.4 and 11.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42400
1 * 42400, recombinant Ara2p, SDS-PAGE
52000
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1 * 52000, SDS-PAGE, PAGE with 6.2 M urea
60000
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PAGE
245000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 7.5
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10663
7 - 8
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285772
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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half-life of partially purified enzyme 2.25 to 2.4 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, almost complete inactivation
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freeze-drying: inactivation
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redissolution of (NH4)2SO4-precipitates, complete inactivation
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
col-16a; mutants col-15a
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native enzyme to near homogeneity
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partial
recombinant His6-tagged Ara2p from Escherichia coli by anion exchange and nickel affinity chromatography to homogeneity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene ARA1, co-overexpression of the enzyme and the D-arabinono-1,4-lactone oxidase and/or L-galactono-1,4-lactone dehydrogenase in Saccharomyces cerevisiae strains result in overproduction and accumulation of vitamin C in the culture medium, while overexpression of the D-arabinose dehydrogenase alone does not alter the L-ascorbate content, overview
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gene YMR041c or ARA2, phylogenetic tree, expression of His6-tagged Ara2p in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis