Information on EC 1.1.1.112 - indanol dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.112
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RECOMMENDED NAME
GeneOntology No.
indanol dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
indan-1-ol + NAD(P)+ = indanone + NAD(P)H + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
indan-1-ol:NAD(P)+ 1-oxidoreductase
3(20)alpha-Hydroxysteroids are also oxidized, more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-43-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2,3,4-tetrahydro-1-naphthol + NAD(P)+
1,2,3,4-tetrahydro-1-naphthone + NAD(P)H
show the reaction diagram
1-acenaphthenol + NAD(P)+
1-acenaphthenone + NAD(P)H
show the reaction diagram
1-tetralol + NAD(P)+
1-tetralone + NAD(P)H
show the reaction diagram
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195% activity compared to indan-(RS)-1-ol
-
?
19-nortestosterone + NAD(P)+
estr-4-en-3,17-dione + NADH
show the reaction diagram
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isoenzymes 3-5
-
?
2,3-butanedione + NAD(P)H
2,3-butanediol + NAD(P)+
show the reaction diagram
-
-
-
?
2,3-heptanedione + NAD(P)H
2,3-heptanediol + NAD(P)+
show the reaction diagram
-
-
-
?
2-nitrobenzaldehyde + NAD(P)H
2-nitrobenzyl alcohol + NAD(P)+
show the reaction diagram
-
-
-
?
3-nitrobenzaldehyde + NAD(P)H
3-nitrobenzyl alcohol + NAD(P)+
show the reaction diagram
-
-
-
?
4-benzoylpyridine + NADH
4-(benzyl alcohol)pyridine + NAD+
show the reaction diagram
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isoenzymes 1,2,3,5,6
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?
4-nitroacetophenone + NADH
(1-hydroxyethyl)-4-nitro-benzene + NAD+
show the reaction diagram
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isoenzymes 1,2,3,5
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?
4-nitrobenzaldehyde + NAD(P)H
4-nitrobenzyl alcohol + NAD(P)+
show the reaction diagram
5alpha-androstan-3-beta,17beta-diol + NAD+
5alpha-androstan-3beta-ol-17-one + NADH
show the reaction diagram
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isoenzymes 2-6
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?
5alpha-androstan-3alpha,17beta-diol + NAD+
5alpha-androstan-3alpha-ol-17-one + NADH
show the reaction diagram
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isoenzymes 1-6
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?
5alpha-androstan-3alpha-ol-17-one + NAD+
5alpha-androstan-3,17-dione + NADH + H+
show the reaction diagram
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isoenzymes 4-5
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?
5alpha-androstan-3alpha-ol-17-one + NADH
5alpha-androstan-3alpha,17alpha-diol + NAD+
show the reaction diagram
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isoenzymes 2,3,4,6
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?
5alpha-androstan-3beta-ol-17-one + NAD+
5alpha-androstan-3,17-dione + NADH + H+
show the reaction diagram
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isoenzymes 2-3
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?
5alpha-androstane-3,17-dione + NAD(P)H
5alpha-androstane-3alpha-ol-17-one + NAD(P)+
show the reaction diagram
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-
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5alpha-dihydrotestosterone + NAD(P)+
5alpha-dihydro-17-oxo-testosterone + NADH
show the reaction diagram
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isoenzymes 2-6
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?
5alpha-dihydrotestosterone + NAD+
5alpha-17-oxo-dihydrotestosterone + NADH + H+
show the reaction diagram
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isoenzymes 1-6
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?
5beta-androstan-3alpha,17beta-diol + NAD+
5beta-androstan-3alpha-ol-17-one + NADH
show the reaction diagram
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isoenzymes 1-7
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?
5beta-androstan-3alpha-ol-17-one + NAD+
5beta-androstan-3,17-dione + NADH
show the reaction diagram
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isoenzymes 3-4
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?
5beta-androstan-3alpha-ol-17-one + NADH
5beta-androstan-3alpha,17alpha-diol + NAD+
show the reaction diagram
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isoenzymes 2,3,4,6,7
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?
5beta-androstan-3beta,17beta-diol + NAD+
5alpha-androstan-3beta-ol-17-one + NADH
show the reaction diagram
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isoenzymes 2-6
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?
5beta-androstan-3beta-ol-17-one + NAD+
5beta-androstan-3,17-dione + NADH
show the reaction diagram
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isoenzyme 5
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?
5beta-androstane-3,17-dione + NAD(P)H
5beta-androstane-3alpha-ol-17-one + NAD(P)+
show the reaction diagram
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-
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5beta-dihydrotestosterone + NAD(P)+
5beta-dihydro-17-oxo-testosterone + NADH
show the reaction diagram
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isoenzymes 2-6
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?
5beta-dihydrotestosterone + NAD+
5beta-17-oxo-dihydrotestosterone + NADH + H+
show the reaction diagram
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isoenzymes 1-6
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?
5beta-pregnan-3-alpha,20-alpha-diol + NAD(P)+
5beta-pregnan-3,20-dione + NAD(P)H
show the reaction diagram
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-
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?
5beta-pregnan-3-alpha-ol-20-one + NAD(P)+
5beta-pregnan-3,20-dione + NAD(P)H
show the reaction diagram
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?
5beta-pregnane-3,20-dione + 2 NAD(P)H + 2 H+
5beta-pregnan-3alpha,20-alpha-diol + 2 NAD(P)+
show the reaction diagram
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5beta-pregnane-3alpha-ol-20-one + NAD(P)H + H+
5beta-pregnan-3alpha,20-alpha-diol + NAD(P)+
show the reaction diagram
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5beta-pregnane-3beta-ol-20-one + NAD(P)H + H+
5beta-pregnan-3,20-diol + NAD(P)+
show the reaction diagram
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9-fluorenol + NAD(P)+
9-fluorenone + NAD(P)H
show the reaction diagram
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112% activity compared to indan-(RS)-1-ol
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?
benzene dihydrodiol + NAD(P)+
o-benzoquinone + NAD(P)H
show the reaction diagram
benzosuberol + NAD(P)+
benzosuberone + NAD(P)H
show the reaction diagram
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12% activity compared to indan-(RS)-1-ol
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?
camphorquinone + NAD(P)H + H+
camphor-1,4-diol + NAD(P)+
show the reaction diagram
chenodeoxycholic acid + NAD(P)+
7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H
show the reaction diagram
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-
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?
cyclohex-2-en-1-ol + NAD(P)+
cyclohex-2-en-1-one + NAD(P)H
show the reaction diagram
cyclohexanol + NAD(P)+
cyclohexanone + NAD(P)H
show the reaction diagram
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isoenzymes 1-7
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?
cyclohexen-3-ol + NAD(P)+
cyclohexen-3-one + NAD(P)H
show the reaction diagram
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103% activity compared to indan-(RS)-1-ol
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?
dehydrocholic acid + NAD(P)H
cholic acid + NAD(P)+
show the reaction diagram
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?
DL-glyceraldehyde + NADH
glycerol + NAD+
show the reaction diagram
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isoenzymes 1-6
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?
glycochenodeoxycholic acid + NAD(P)+
N[7alpha-hydroxy-3-oxo-5beta-cholan-24-oyl]glycine + NAD(P)H
show the reaction diagram
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?
glycolithocholic acid + NAD(P)+
N[3-oxo-5beta-cholan-24-oyl]glycine + NAD(P)H
show the reaction diagram
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?
indan-(RS)-1-ol + NAD(P)+
indan-1-one + NAD(P)H
show the reaction diagram
indan-(S)-1-ol + NAD(P)+
indan-1-one + NAD(P)H
show the reaction diagram
indan-2-ol + NAD(P)+
indan-2-one + NAD(P)H
show the reaction diagram
lithocholic acid + NAD(P)+
3-oxo-5beta-cholan-24-oic acid + NAD(P)H
show the reaction diagram
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r
progesterone + NAD(P)H
3alpha-hydroxy-4-pregnen-20-one + NAD(P)+
show the reaction diagram
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pyridine-4-aldehyde + NADH
pyridine-4-alcohol + NAD+
show the reaction diagram
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isoenzymes 1-7
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?
taurolithocholic acid + NAD(P)+
2[[7alpha,12alpha-dihydroxy-3,24-dioxo-5beta-cholan-24-yl]amino]ethanesulfonic acid + NAD(P)H
show the reaction diagram
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-
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?
testosterone + NAD(P)+
androst-4-ene-3,17-dione + NADH + H+
show the reaction diagram
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isoenzymes 3-6
?
trans-1,2-dihydroxy-1,2-dihydronaphthalene + NAD(P)+
1,2-naphthoquinone + NAD(P)H
show the reaction diagram
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?
trans-acenaphthene-1,2-diol + NAD(P)+
acenaphtene-1,2-dione + NAD(P)H
show the reaction diagram
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100% activity compared to indan-(RS)-1-ol
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2,2'-bipyridine
4,4'-Bipyridine
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competitive inhibition vs. indanol
6alpha-Methylprednisolone
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0.1 mM, oxidation of acenaphthenol: 0% inhibition of isozyme 1, 40% inhibition of isozyme 2, 23%,inhibition of isozyme 3, 89% inhibition of isozyme 4, 14% inhibition of isozyme 5, 22% inhibition of isozyme 6 and 18% inhibition of isoenzyme 7, oxidation of 5alpha-androstane-3alpha,17beta-diol: 7% inhibition of isozyme 1, 41% inhibition of isozyme 2, 30%,inhibition of isozyme 3, 86% inhibition of isozyme 4, 11% inhibition of isozyme 5, 16% inhibition of isozyme 6 and 20% inhibition of isoenzyme 7
8-hydroxyquinoline
betamethazone
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0.05 mM, oxidation of acenaphthenol: 32% inhibition of isozyme 1, 16% inhibition of isozyme 2, 90%,inhibition of isozyme 3, 95% inhibition of isozyme 4, 27% inhibition of isozyme 5, 22% inhibition of isozyme 6 and 0% inhibition of isoenzyme 7, oxidation of 5alpha-androstane-3alpha,17beta-diol: 14% inhibition of isozyme 1, 26% inhibition of isozyme 2, 97%,inhibition of isozyme 3, 96% inhibition of isozyme 4, 30% inhibition of isozyme 5, 22% inhibition of isozyme 6 and 6% inhibition of isoenzyme 7
Cibacron blue
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noncompetitive inhibition vs. indanol
Dienstrol
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0.01 mM, oxidation of acenaphthenol: 27% inhibition of isozyme 1, 84% inhibition of isozyme 2, 99%,inhibition of isozyme 3, 94% inhibition of isozyme 4, 0% inhibition of isozyme 5, 66% inhibition of isozyme 6 and 0% inhibition of isoenzyme 7, oxidation of 5alpha-androstane-3alpha,17beta-diol: 27% inhibition of isozyme 1, 85% inhibition of isozyme 2, 99%,inhibition of isozyme 3, 97% inhibition of isozyme 4, 0% inhibition of isozyme 5, 51% inhibition of isozyme 6 and 0% inhibition of isoenzyme 7
diethylstilbestrol
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0.01 mM, oxidation of acenaphthenol: 25% inhibition of isozyme 1, 68% inhibition of isozyme 2, 94%,inhibition of isozyme 3, 90% inhibition of isozyme 4, 6% inhibition of isozyme 5, 44% inhibition of isozyme 6 and 44% inhibition of isoenzyme 7, oxidation of 5alpha-androstane-3alpha,17beta-diol: 23% inhibition of isozyme 1, 69% inhibition of isozyme 2, 87%,inhibition of isozyme 3, 99% inhibition of isozyme 4, 0% inhibition of isozyme 5, 49% inhibition of isozyme 6 and 47% inhibition of isoenzyme 7
Flufenamic acid
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0.1 mM, oxidation of acenaphthenol: 54% inhibition of isozyme 1, 47% inhibition of isozyme 2, 64%,inhibition of isozyme 3, 60% inhibition of isozyme 4, 50% inhibition of isozyme 5, 57% inhibition of isozyme 6 and 38% inhibition of isoenzyme 7, oxidation of 5alpha-androstane-3alpha,17beta-diol: 46% inhibition of isozyme 1, 45% inhibition of isozyme 2, 47%,inhibition of isozyme 3, 49% inhibition of isozyme 4, 58% inhibition of isozyme 5, 46% inhibition of isozyme 6 and 43% inhibition of isoenzyme 7
Hexestrol
indomethacin
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0.1 mM, oxidation of acenaphthenol: 20% inhibition of isozyme 1, 49% inhibition of isozyme 2, 55%,inhibition of isozyme 3, 78% inhibition of isozyme 4, 64% inhibition of isozyme 5, 59% inhibition of isozyme 6 and 3% inhibition of isoenzyme 7
Medroxyprogesterone acetate
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competitive inhibition vs. indanol
progesterone
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competitive inhibition vs. indanol
pyrazole
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1 mM, 39% inhibition
Zomepirac
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0.1 mM, oxidation of acenaphthenol: 69% inhibition of isozyme 1, 46% inhibition of isozyme 2, 71%,inhibition of isozyme 3, 78% inhibition of isozyme 4, 69% inhibition of isozyme 5, 72% inhibition of isozyme 6 and 56% inhibition of isoenzyme 7, oxidation of 5alpha-androstane-3alpha,17beta-diol: 70% inhibition of isozyme 1, 68% inhibition of isozyme 2, 68%,inhibition of isozyme 3, 56% inhibition of isozyme 4, 68% inhibition of isozyme 5, 71% inhibition of isozyme 6 and 66% inhibition of isoenzyme 7
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0008
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cytosolic fraction, cofactor NADP+
0.001
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cytosolic fraction, cofactor NAD+
0.0079
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mitochondrial fraction, cofactor NADP+
0.0126
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microsomal fraction, cofactor NADP+
0.0181
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microsomal fraction, cofactor NAD+
0.118
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mitochondria fraction, cofactor NAD+
0.74
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1.13
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isoenzyme 5, substrate 1-acenaphthenol
2.02
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isoenzyme 1, substrate 1-acenaphthenol
2.93
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isoenzyme 4, substrate 1-acenaphthenol
4.03
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isoenzyme 2, substrate 1-acenaphthenol
4.09
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isoenzyme 3, substrate 1-acenaphthenol
4.33
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isoenzyme 6, substrate 1-acenaphthenol
7.77
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isoenzyme 7, substrate 1-acenaphthenol
11.1
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1-acenaphthenol as substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 10
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isoenzyme 5
9 - 10
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cofactor NAD+
9.8
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second maximum at pH 8.6
10 - 10.5
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cofactor NADP+
10
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with lithocholic acid as substrate
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 50
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linear increase
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
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gel filtration, isoenzyme 2
31000
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gel filtration, isoenzyme 3; gel filtration, isoenzyme 7
32000
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gel filtration, isoenzyme 1
33000
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gel filtration, isoenzyme 5
34000
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gel filtration, isoenzyme 4
38000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 36000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2 - 10.5
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10 min at 25°C
285758
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48
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60% activity lost after 10 min, pH 7.0
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 150 mM KCl, more than 1 mg/ml enzyme concentration, 4 months, little loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, CM-cellulose, Sephadex G-75, Sephadex G-100
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ammonium sulfate, CM-Sephadex, Sephadex G-1000, DEAE-Sephacel, Blue-Sepharose, hydroxyapatite, DEAE-Sephacel, isoelectric focusing, CM-Sepharose, isoenzymes 1-7
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ammonium sulfate, Sephadex G-100, Blue Sepharose, hydroxyapatite, chromatofocusing
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