Information on EC 1.1.1.106 - pantoate 4-dehydrogenase

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The expected taxonomic range for this enzyme is: Pseudomonas

EC NUMBER
COMMENTARY hide
1.1.1.106
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RECOMMENDED NAME
GeneOntology No.
pantoate 4-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-pantoate + NAD+ = (R)-4-dehydropantoate + NADH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pantothenate and CoA biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(R)-pantoate:NAD+ 4-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-38-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-pantoate + NAD+
(R)-4-dehydropantoate + NADH
show the reaction diagram
2-oxo-pantoate + NAD+
2-oxo-4-dehydropantoate
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R)-pantoate + NAD+
(R)-4-dehydropantoate + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
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1 mM, 50% inhibition after 4.6 min, half-inactivation time increases to 7.0 min in the presence of 20 mM D-pantoate
iodacetic acid
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1 mM, 50% inhibition after 14 min, half-inactivation time increases to 29.0 min in the presence of 20 mM D-pantoate, 70% inhibition within 50 min at pH 7.2, 20 mM pantoate decreases inactivation from 70 to 30%
p-chloromercuribenzoate
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0.1 mM, 85% inhibition after 15 min, 200 mM 2-mercaptoethanol restores 70% of enzyme activity within 10 min, 20 mM D-pantoate and 1 mM NAD+ prevent inactivation when added simultaneously
Phenylglyoxal
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5.3 mM, 70% inhibition in borate buffer, 92% inhibition in NaHCO3 buffer within 10 min, complete inactivation after prolonged incubation with phenylglyoxal, NAD+ and D-pantoate increase half-inactivation time from 6 min without substrates to 40 and 17 min respectively
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.033
D-pantoate
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at pH 10.0
0.5
ketopantoate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
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linear activity increase
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80000
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ultracentrifugation
86000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable without 2-mercaptoethanol, NAD+ stabilizes to a certain extent, the enzyme is unstable below pH 5.5 and above pH 10.0 even in the presence of NAD+
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 2 mM potassium phosphate, pH 7.0, 0.01% EDTA, 1 mM mercaptoethanol, several months, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
50°C, protamine sulfate, calcium phosphate gel, ammonium sulfate, 50°C
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ammonium sulfate, 5'-AMP-Sepharose 4B
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ammonium sulfate, 50°C, gel filtration, hydroxyapatite, 60°C, gel filtration
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