3.2.1.23: beta-galactosidase
This is an abbreviated version!
For detailed information about beta-galactosidase, go to the full flat file.
Word Map on EC 3.2.1.23
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3.2.1.23
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lacz
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adenovirus
-
lysosomal
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lactose
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endothelial
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oligosaccharide
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artery
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phage
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adenovirus-mediated
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beta-glucosidase
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immunoassay
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retroviral
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beta-glucuronidase
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herpes
-
lambda
-
simplex
-
luciferase
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cytomegalovirus
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neuraminidase
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viruses
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hydrolases
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bacteriophage
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glycosidases
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gm1
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liposome
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ganglioside
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p16ink4a
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alpha-galactosidase
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alpha-mannosidase
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osteogenic
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vaccinia
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adeno-associated
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replication-defective
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n-acetyl-beta-glucosaminidase
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runx2
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sialidase
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alpha-glucosidase
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sialic
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galactosylation
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plaque-forming
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osteoblast
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kluyveromyces
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telomerase
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analysis
-
degradation
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diagnostics
-
lysogens
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beta-hexosaminidase
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keratan
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vector-mediated
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biotechnology
-
molecular biology
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synthesis
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environmental protection
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ganciclovir
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nutrition
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transglycosylation
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medicine
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industry
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nonviral
-
food industry
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biofuel production
- 3.2.1.23
- lacz
- adenovirus
- lysosomal
- lactose
- endothelial
- oligosaccharide
- artery
- phage
-
adenovirus-mediated
- beta-glucosidase
-
immunoassay
-
retroviral
- beta-glucuronidase
-
herpes
- lambda
- simplex
- luciferase
- cytomegalovirus
- neuraminidase
- viruses
- hydrolases
- bacteriophage
- glycosidases
- gm1
- liposome
- ganglioside
- p16ink4a
- alpha-galactosidase
- alpha-mannosidase
-
osteogenic
- vaccinia
-
adeno-associated
-
replication-defective
- n-acetyl-beta-glucosaminidase
- runx2
- sialidase
- alpha-glucosidase
-
sialic
-
galactosylation
-
plaque-forming
-
osteoblast
- kluyveromyces
- telomerase
- analysis
- degradation
- diagnostics
-
lysogens
- beta-hexosaminidase
- keratan
-
vector-mediated
- biotechnology
- molecular biology
- synthesis
- environmental protection
- ganciclovir
- nutrition
-
transglycosylation
- medicine
- industry
-
nonviral
- food industry
- biofuel production
Reaction
Synonyms
Aabeta-gal, Acid beta-galactosidase, B-GAL, BbgI, BbgII, BbgIII, BbgIV, beta galactosidase, beta-D-galactohydrolase, beta-D-galactosidase, beta-D-galactoside galactohydrolase, beta-D-glactanase, beta-D-lactosidase, beta-gal, beta-Gal 1, beta-Gal 2, beta-Gal II, beta-galactosidase, beta-galactosidase I, beta-galactosidase II, beta-galactosidase III, beta-galactosidase IV, beta-galase, beta-lactosidase, betagal, betaGly4, betaGS, BGA, BgaB, BgaBM, bgaD, BgaH, BGAL, BGAL1, BGAL10, BGAL11, BGAL12, BGAL13, BGAL14, BGAL15, BGAL16, BGAL17, BGAL2, BGAL3, BGAL4, BGAL5, BGAL6, BGAL7, BGAL8, BGAL9, BgalA, BgAP, BgaS, BgaX, BGL1, BglAp, BGT I, BGT II, cold-active beta-galactosidase, CTP-beta-gal, driselase, EABase, endo-beta-galactosidase, Exo-(1-->4)-beta-D-galactanase, exo-beta-(1->3)-D-galactanase, Gal-2, Gal-5, GAL1, Gal2, Gal3, Gal4, GALA, galactanase, galactosidase, galactosidase, beta, GALB, gherkin lactase, H-BgaS, hcbetagal, Hlac_2868, Hydrolact, LacA, LACS, lactase, lactase phlorizin hydrolase, lactosylceramidase II, Lactozym, Lactozym 3000L, LPH, Maxilact, Maxilact-L/2000, MeBglD2, More, ONPGase, Oryzatym, p-nitrophenyl beta-galactosidase, PF1208, PRGH1, S 2107, SA-beta-GAL, SA-betagal, senescence-associated beta-galactosidase, SPD_0065 protein, SR12 protein, Ss beta-gal, SSO3019, SSU0587, Sumiklat, Trilactase, XC1214, XG-specific beta-galactosidase, YesZ, YH4502, ZD410
ECTree
3.2.1.23 beta-galactosidaseAdvanced search results
results (
results (KM Value
KM Value on EC 3.2.1.23 - beta-galactosidase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.031 - 0.064
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside
7.3
2-nitrophenyl-beta-D-fucopyranoside
pH 6.5, 65°C, wild-type enzyme
0.00008
3,4-dinitrophenyl beta-D-galactopyranoside
-
pH 7.0, 30°C, mutant E184A, glycohydrolase activity
5.2
4-nitrophenyl-alpha-L-arabinopyranoside
pH 6.5, 65°C, wild-type enzyme
1.76
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc
pH 4.5, 60°C
2.41
beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc
pH 4.5, 60°C
0.031
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside
-
mutant enzyme E354A, in 20 mM sodium acetate buffer at pH 6.0, 37°C
0.059
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside
-
mutant enzyme E467A, in 20 mM sodium acetate buffer at pH 6.0, 37°C
0.061
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside
-
mutant enzyme D429A, in 20 mM sodium acetate buffer at pH 6.0, 37°C
0.064
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside
-
wild type enzyme, in 20 mM sodium acetate buffer at pH 6.0, 37°C
0.04
2-nitrophenyl beta-D-galactopyranoside
wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, pH 7.0 at 25°C
0.06
2-nitrophenyl beta-D-galactopyranoside
mutant enzyme H418N, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C
0.0836
2-nitrophenyl beta-D-galactopyranoside
-
enzyme encapsulated in a silica matrix
0.9
2-nitrophenyl beta-D-galactopyranoside
-
at pH 7.5 and 55°C
0.97
2-nitrophenyl beta-D-galactopyranoside
-
in 50 mM glycine-HCl, pH 3.2, at 37°C
1.21
2-nitrophenyl beta-D-galactopyranoside
-
at pH 6.0 and 80°C
2.805
2-nitrophenyl beta-D-galactopyranoside
-
pH 9, 50°C, Vmax: 0.03745 mM/min/mg
2.87
2-nitrophenyl beta-D-galactopyranoside
-
mutant FMNP01Gal-2, pH 6.5, 40°C
3.76
2-nitrophenyl beta-D-galactopyranoside
mutant S259F/P261T/L262S/G263S, pH 5.5, 42°
6.68
2-nitrophenyl beta-D-galactopyranoside
-
wild-type, pH 6.5, 40°C
30.6
2-nitrophenyl beta-D-galactopyranoside
mutant enzyme H418F, in 30 mM TES buffer, 10 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C
104
2-nitrophenyl beta-D-galactopyranoside
mutant enzyme H418E, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C
0.0381
2-nitrophenyl beta-D-galactoside
mutant enzyme W806F, at pH 5.0 and 60°C
0.0522
2-nitrophenyl beta-D-galactoside
wild type enzyme, at pH 5.0 and 60°C
0.87
2-nitrophenyl beta-D-galactoside
pH 7.2, 22°C, the enzyme also shows beta-fucosidase activity
0.11
2-nitrophenyl-beta-D-galactopyranoside
pH 6.5, 65°C, mutant E157G
0.16
2-nitrophenyl-beta-D-galactopyranoside
wild type enzyme, in 100 mM sodium acetate buffer pH 5.5, at 45°C
0.17
2-nitrophenyl-beta-D-galactopyranoside
mutant enzyme E491A, in HEPES buffer pH 6.5, at 45°C
0.73
2-nitrophenyl-beta-D-galactopyranoside
pH 6.5, 30°C
0.8
2-nitrophenyl-beta-D-galactopyranoside
-
pH 6.5, 40°C, recombinant hybrid enzyme
0.8
2-nitrophenyl-beta-D-galactopyranoside
-
pH 6.5, 40°C, recombinant hybrid enzyme
1.5
2-nitrophenyl-beta-D-galactopyranoside
-
pH 6.5, 40°C, wild-type enzyme
2.7
2-nitrophenyl-beta-D-galactopyranoside
pH 8.0, 10°C, recombinant enzyme
4.1
2-nitrophenyl-beta-D-galactopyranoside
pH 6.8, 37°C
4.8
2-nitrophenyl-beta-D-galactopyranoside
pH 6.5, 65°C, mutant E313G
5.22
2-nitrophenyl-beta-D-galactopyranoside
in glycine-HCl buffer (pH 1.5)
5.5
2-nitrophenyl-beta-D-galactopyranoside
pH 6.5, 65°C, wild-type enzyme
21.68
2-nitrophenyl-beta-D-galactopyranoside
-
pH 7.5, 30°C, isozyme BGT II
0.59
4-methylumbelliferyl beta-D-galactoside
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fibroblast enzyme of GM1 gangliosidosis heterozygotes
0.65
4-methylumbelliferyl beta-D-galactoside
-
leukocyte enzyme of GM1 gangliosidosis heterozygotes
0.46
4-nitrophenyl beta-D-fucopyranoside
pH 4.6, 37°C, native enzyme
0.47
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, without additive
0.51
4-nitrophenyl beta-D-fucopyranoside
pH 4.6, 37°C, recombinant enzyme
0.65
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, additive: 250 mM D-galactose
0.98
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, additive: 25 mM cellobiose
1.3
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, additive: 250 mM D-xylose
3.93
4-nitrophenyl beta-D-fucopyranoside
pH 6.0, 60°C, additive: 250 mM D-glucose
0.04
4-nitrophenyl beta-D-galactopyranoside
mutant enzyme H418N, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C
0.11
4-nitrophenyl beta-D-galactopyranoside
-
at pH 6.0 and 80°C
0.12
4-nitrophenyl beta-D-galactopyranoside
wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, pH 7.0 at 25°C
0.64
4-nitrophenyl beta-D-galactopyranoside
pH 4.6, 37°C, native enzyme
0.68
4-nitrophenyl beta-D-galactopyranoside
pH 4.6, 37°C, recombinant enzyme
6.18
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, additive: 250 mM D-galactose
7.44
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, additive: 25 mM cellobiose
7.65
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, without additive
8.21
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, additive: 250 mM D-xylose
13.2
4-nitrophenyl beta-D-galactopyranoside
mutant enzyme H418E, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C
13.3
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 60°C, additive: 250 mM D-glucose
20.8
4-nitrophenyl beta-D-galactopyranoside
mutant enzyme H418F, in 30 mM TES buffer, 10 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C
0.49
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, without additive
0.53
4-nitrophenyl beta-D-glucopyranoside
pH 4.6, 37°C, native enzyme
0.58
4-nitrophenyl beta-D-glucopyranoside
pH 4.6, 37°C, recombinant enzyme
0.77
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, additive: 25 mM cellobiose
0.83
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, additive: 250 mM D-galactose
2.22
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, additive: 250 mM D-xylose
2.43
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 60°C, additive: 250 mM D-glucose
0.002
4-nitrophenyl-beta-D-galactopyranoside
-
pH 7.0, 30°C, mutant E184A, glycohydrolase activity
0.032
4-nitrophenyl-beta-D-galactopyranoside
pH 3.5, 60°C, recombinant isozyme OsGal1
0.05
4-nitrophenyl-beta-D-galactopyranoside
-
pH 7.0, 30°C, wild-type enzyme, glycohydrolase activity
4.9
4-nitrophenyl-beta-D-galactopyranoside
pH 6.5, 65°C, wild-type enzyme
7.1
4-nitrophenyl-beta-D-galactopyranoside
pH 6.8, 37°C
0.94
lactose
mutant enzyme E491A, in HEPES buffer pH 6.5, at 45°C
0.98
lactose
wild type enzyme, in 100 mM sodium acetate buffer pH 5.5, at 45°C
34
lactose
-
transferase reaction, in presence of 400 mM myo-inositol, 1 mg/ml bovine serum albumin, 50 mM glycine/HCl, pH 3.0
345
myo-inositol
-
transferase reaction, in presence of 50 mM glycine/HCl buffer, pH 3.0, 80 mM lactose, 0.5 mg/ml alpha-lactalbumin
0.365
o-nitrophenyl-beta-D-galactoside
-
wild-type enzyme, at 11-45°C
0.371
o-nitrophenyl-beta-D-galactoside
-
mutant enzyme L317F, 11-45°C
0.45
o-nitrophenyl-beta-D-galactoside
-
beta-D-galactoside galactohydrolase pH 4.2
0.479
o-nitrophenyl-beta-D-galactoside
-
mutant enzyme P429S, at 11-45°C
0.58
o-nitrophenyl-beta-D-galactoside
-
at 50°C, in presence of 10 mM Mg2+
0.64
o-nitrophenyl-beta-D-galactoside
-
at 25°C, in presence of 10 mM Mg2+
0.98
o-nitrophenyl-beta-D-galactoside
-
pH 6.5, 30°C, strain L461
1.17
o-nitrophenyl-beta-D-galactoside
-
enzyme immobilized on sucrose-polycarolein
1.25
o-nitrophenyl-beta-D-galactoside
-
beta-D-galactoside galactohydrolase pH 4.5
1.56
o-nitrophenyl-beta-D-galactoside
-
enzyme immobilized on sucrose-polycarolein
1.61
o-nitrophenyl-beta-D-galactoside
Saccharomyces fragilis
-
enzyme immobilized on sucrose-polyacrolein
1.65
o-nitrophenyl-beta-D-galactoside
-
enzyme immobilized on sucrose sulfate-polyacrolein
0.34
p-nitrophenyl-beta-D-galactoside
-
97000 da enzyme form and 84000 Da enzyme form
0.37
p-nitrophenyl-beta-D-galactoside
-
at 50°C, in presence of 10 mM Mg2+
0.39
p-nitrophenyl-beta-D-galactoside
-
at 25°C, in presence of 10 mM Mg2+
additional information
additional information
-
effect of cations on the Km-value
-
additional information
additional information
-
Km-values of chimeric enzymes
-
additional information
additional information
-
influence of temperature on Km-value of o-nitrophenyl-beta-D-galactoside
-
additional information
additional information
-
kinetics and thermodynamics
-
additional information
additional information
-
enzyme displays non-Michaelis-Menten reaction kinetics with substrate activation for lactose, which is explained by simultaneous reactions of hydrolysis and transgalactosylation
-
additional information
additional information
-
detailed kinetics, overview
-
additional information
additional information
-
kinetic modelling of the thermal and pH inactivation of the free and an immobilized form of the enzyme, detailed overview
-
additional information
additional information
-
kinetics of the thioglycoligase activity of mutant E184A, overview
-
additional information
additional information
-
temperature dependence of kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
discussion of precautions and recommendations for measuring values of the rate constants and the associated enthalpies and entropies of enzymes with two catalytic steps by determining the effects of temperature on the kcat values
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