2.7.7.B5: adenylyltransferase ThiI
This is an abbreviated version!
For detailed information about adenylyltransferase ThiI, go to the full flat file.
Reaction
Synonyms
ThiI
ECTree
2.7.7.B5 adenylyltransferase ThiIAdvanced search results
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results (Engineering
Engineering on EC 2.7.7.B5 - adenylyltransferase ThiI
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
C207S
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using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis
C344S
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using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis
C456S
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using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis and for thiazole synthesis
D189A
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using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis
K321M
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using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis
additional information
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construction of two truncated forms of ThiI containing the N-terminal domain including NFLD and THUMP, and the C-terminal domain including the PP-loop and RLD domains, respectively. The N-domain can bind with both tRNAPhe and TPHE39A and recognizes the acceptor-stem region, whereas the C-domain cannot. The C-domain also affects RNA binding by its enthalpically favorable, but entropically unfavorable, contribution. The C-domain induces a conformation change in tRNAPhe
