2.7.7.B22: transposase

This is an abbreviated version!
For detailed information about transposase, go to the full flat file.

Reaction

binds to the ends of a transposon (DNA that moves) and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism =

Synonyms

Ac transposase, AcTPase, APM_2825, Buster, DbuzGalileo, DDE-type integrase/transposase/recombinase, DDE_Tnp_1_7 domain-containing protein, DNA transposase, ECO103_3558, Galileo transposase, hAT transposase, hAT-transposase 1, Hermes, Hermes transposase, Hsmar1 transposase, ICE6013, IS10 transposase, IS1341-type transposase, IS1634 transposase, IS200 transposase, IS30-like transposase, IS608, IS711, ISC1041, Kat1, maize activator transposase, mariner transposase, Mbar_A1742, PGM, piggyBac transposase, PiggyMac, Rev 1 transposase, Rev 2 transposase, SB transposase, SB100X, SETMAR, sleeping beauty transposase, SSO1474, Ta0474, Tgf2 TPase, Tgf2 transposase, Tgf2TPase, Tn4430 TnpA, Tn5, Tn5 transposase, Tn7 transposase, TnpA, TnpA 155-amino acid transposase, TnpA transposase, TnsA, TnsB, Tol2, Tpase, transposase for transposon Tn4430, transposase for transposon Tn5, wbk IS transposase, wbkA flanking transposase, wbkA-flanking IS

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.7 Nucleotidyltransferases

                2.7.7.B22 transposase

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Results	in table
2	Activating Compound
14	AA Sequence
10	Application
22	Cloned(Commentary)
6	Crystallization (Commentary)
460	Disease/ Diagnostics
3	Expression
52	General Information
9	Localization
5	Metals/Ions
5	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
47	Organism
2	pH Optimum
35	Protein Variants
6	Purification (Commentary)
1	Reaction
45	Reference
16	Substrates and Products (Substrate)
17	Subunits
97	Synonyms
3	Temperature Optimum [°C]
1	Temperature Stability [°C]

Purification

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Purification on EC 2.7.7.B22 - transposase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Saccharolobus solfataricus

Q97Y68

725373

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography

Zea mays

P08770

739041

recombinant N-terminally thioredoxinA-6xHis-tagged wild-type and mutant enzymes from Escherichia coli strain RosettaII(DE3) by nickel affinity chromatography

Homo sapiens

-

739149

recombinant soluble His6-tagged enzyme from Escherichia coli strain Rossetta 1 (DE3) by nickel affinity chromatography, identification of the recombinant enzyme by MALDI-TOF-TOF tandem MS analysis

Carassius auratus

H9AER4

739044

recombinant TnsC1-85 MBP-fusion protein from Escherichia coli strain BL21Star(DE3) pET101/DTOPO-TnsD1–309His, co-purification of the TnsC and TnsD fragments by nickel affinity chromatography, TnsC1-85 interacts directly with TnsD1-309

Escherichia coli

P13988; P13989

739543

soluble His-tagged PAI subdomain of SB transposase from Escherichia coli strain BL21-A1 by nickel affinity chromatography

Homo sapiens

-

739500