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2.7.7.B16: DNA primase

This is an abbreviated version!
For detailed information about DNA primase, go to the full flat file.

Word Map on EC 2.7.7.B16

Reaction

dNTP
+ n dNTP =
dN(pdN)n
+ n diphosphate

Synonyms

archaeal eukaryotic-type primase, DNA primase-polymerase, DnaG primase, Mjpri, Pabp41, Pabp46, pIT3 replication protein, PolpTN2, PriL, PriS, PriSL, PriX, Sso core primase, SsoPriSL

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.B16 DNA primase

Temperature Range

Temperature Range on EC 2.7.7.B16 - DNA primase

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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
coupled assay with DNA polymerase B
45 - 75
45°C: about 40% of maximal activity, 75°C: about 40% of maximal activity
55 - 70
polymerization reactions on C35 or C34ddC with rGTP. Both the size and the quantity of the products on C35 increase, while synthesis on C34ddC drastically decreases, when the reaction temperature is raised from 55°C to 70°C
68 - 80
-
activity range of template?free synthesis, which is highly temperature-dependent
additional information
-
delayed kinetics of the reaction at low temperature