Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.7.8: polyribonucleotide nucleotidyltransferase

This is an abbreviated version!
For detailed information about polyribonucleotide nucleotidyltransferase, go to the full flat file.

Word Map on EC 2.7.7.8

Reaction

RNAn+1
+
phosphate
=
RNAn
+
a nucleoside diphosphate

Synonyms

AtcpPNPase, AtmtPNPase, chloroplast PNPase, cpPNPase, hPNPase(old-35), hPNPaseold-35, nucleoside diphosphate:polynucleotidyl transferase, nucleotidyltransferase, polyribonucleotide, PNP, PNPase, PNPT1, polynucleotide phosphorylase, polyribonucleotide phosphorylase, RNase PH

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.8 polyribonucleotide nucleotidyltransferase

Purification

Purification on EC 2.7.7.8 - polyribonucleotide nucleotidyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
-
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Mono-Q column chromatography
ammonium sulfate, DEAE-cellulose, gel filtration, poly(A)-Sepharose
-
ammonium sulfate, DEAE-cellulose, heparin-Sepharose, DEAE-Sephadex
-
ammonium sulfate, DEAE-cellulose, RNA-sepharose
-
ammonium sulfate, ethanol, calcium phosphate gel, protamine, hydroxylapatite
-
ammonium sulfate, polyethlene glycol 6000, DEAE-Sepharose, QAE-Sephadex, blue-Sepharose, glycerol gradient centrifugation
-
DEAE-cellulose, ammonium sulfate, Sephadex G-200
-
DEAE-cellulose, Sephacryl S-300, Mono Q, poly(A)-Sepharose
-
DEAE-Sephadex, enzyme from healthy and TMV-infected leaves
-
heat, ammonium sulfate, Sephadex G-200, DEAE-cellulose
-
manganese-RNA gel, DEAE-cellulose, ammonium sulfate, partially purified
-
MonoQ colmn chromatography, metal ion affinity chromatography, and Superdex 200 gel filtration
-
phase partition, ammonium sulfate, agarose gel filtration, DEAE-Sephadex
-
phosphocellulose chromatography, enzyme forms I and T
-
phosphocellulose, DEAE-Sephadex, Blue-Sepharose, Sephacryl s-300
-
polyethylene imine, Sepharose 4B, affinity chromatography on oligo(dT)-Sepharose
-
polymin P, DEAE-cellulose, Sephadex G-150, DEAE-cellulose, Sephadex G-200
-
recombinant enzyme, HiTrap Q, HiFlow Phenyl-Sepharose
recombinant N-terminally GST-tagged enzyme fragments from Escherichia coli by glutathione affinity chromatography, of His6-tagged enzyme fragments by nickel affinity chromatography, followed by gel filtration in both procedures
-
recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by nickel affinty chromatography and gel filtration
recombinant PNPase, nitrilotriacetic acid agarose, Mono Q
recombinant wild-type and mutant enzymes from Escherichia coli strain ENS134-3
-
Streptomycin sulfate, ammonium sulfate, Zncl2 precipitation, DEAE-cellulose
-
ultracentrifugation, ammonium sulfate, QAE-Sephadex a-25, DEAE-cellulose, hydroxyapatite, purified enzyme loses poly(A) phosphorylating activity
-