2.7.7.8: polyribonucleotide nucleotidyltransferase
This is an abbreviated version!
For detailed information about polyribonucleotide nucleotidyltransferase, go to the full flat file.
Word Map on EC 2.7.7.8
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2.7.7.8
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rnase
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exoribonuclease
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polya
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ribonuclease
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polymerization
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polyadenylation
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phosphorolysis
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degradosome
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helicase
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exonuclease
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exonucleolytic
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stem-loop
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luteus
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micrococcus
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5'-diphosphate
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rna-binding
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kh
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hfq
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oligoribonucleotides
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rna-degrading
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heteropolymeric
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antibioticus
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dead-box
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lysodeikticus
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primer-independent
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synthesis
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molecular biology
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medicine
- 2.7.7.8
- rnase
- exoribonuclease
- polya
- ribonuclease
- polymerization
-
polyadenylation
-
phosphorolysis
-
degradosome
- helicase
-
exonuclease
-
exonucleolytic
-
stem-loop
- luteus
- micrococcus
- 5'-diphosphate
-
rna-binding
- kh
- hfq
- oligoribonucleotides
-
rna-degrading
-
heteropolymeric
- antibioticus
-
dead-box
- lysodeikticus
-
primer-independent
- synthesis
- molecular biology
- medicine
Reaction
Synonyms
AtcpPNPase, AtmtPNPase, chloroplast PNPase, cpPNPase, hPNPase(old-35), hPNPaseold-35, nucleoside diphosphate:polynucleotidyl transferase, nucleotidyltransferase, polyribonucleotide, PNP, PNPase, PNPT1, polynucleotide phosphorylase, polyribonucleotide phosphorylase, RNase PH
ECTree
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Purification
Purification on EC 2.7.7.8 - polyribonucleotide nucleotidyltransferase
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ammonium sulfate precipitation, Q-Sepharose column chromatography, and Mono-Q column chromatography
ammonium sulfate, ethanol, calcium phosphate gel, protamine, hydroxylapatite
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ammonium sulfate, polyethlene glycol 6000, DEAE-Sepharose, QAE-Sephadex, blue-Sepharose, glycerol gradient centrifugation
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MonoQ colmn chromatography, metal ion affinity chromatography, and Superdex 200 gel filtration
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phase partition, ammonium sulfate, agarose gel filtration, DEAE-Sephadex
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polyethylene imine, Sepharose 4B, affinity chromatography on oligo(dT)-Sepharose
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polymin P, DEAE-cellulose, Sephadex G-150, DEAE-cellulose, Sephadex G-200
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recombinant N-terminally GST-tagged enzyme fragments from Escherichia coli by glutathione affinity chromatography, of His6-tagged enzyme fragments by nickel affinity chromatography, followed by gel filtration in both procedures
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recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by nickel affinty chromatography and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain ENS134-3
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Streptomycin sulfate, ammonium sulfate, Zncl2 precipitation, DEAE-cellulose
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ultracentrifugation, ammonium sulfate, QAE-Sephadex a-25, DEAE-cellulose, hydroxyapatite, purified enzyme loses poly(A) phosphorylating activity
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