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2.7.7.8: polyribonucleotide nucleotidyltransferase

This is an abbreviated version!
For detailed information about polyribonucleotide nucleotidyltransferase, go to the full flat file.

Word Map on EC 2.7.7.8

Reaction

RNAn+1
+
phosphate
=
RNAn
 +
a nucleoside diphosphate

Synonyms

AtcpPNPase, AtmtPNPase, chloroplast PNPase, cpPNPase, hPNPase(old-35), hPNPaseold-35, nucleoside diphosphate:polynucleotidyl transferase, nucleotidyltransferase, polyribonucleotide, PNP, PNPase, PNPT1, polynucleotide phosphorylase, polyribonucleotide phosphorylase, RNase PH

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.8 polyribonucleotide nucleotidyltransferase

Activating Compound

Activating Compound on EC 2.7.7.8 - polyribonucleotide nucleotidyltransferase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP
-
1.6 mM, 30% activation of ADP polymerization
Basic polypeptide
-
peptide from Escherichia coli extract, enhances ADP-phosphate exchange
-
Bis-(3-aminopropyl)-amine
-
optimal polymerization activity requires presence, 2fold increase in activity at 30 mM
citrate
-
a PNPase-mediated response to citrate, and PNPase deletion broadly impacts on the metabolome. PNPase-dependent cells show reduced growth in the presence of increased citrate concentration. In vitro, citrate directly binds and modulates PNPase activity, and the enzyme is inhibited by binding of metal-chelated citrate, predominantly complexed as magnesium-citrate, in the active site at physiological concentrations. In the contrary, metal-free citrate is bound at a vestigial active site, where it stimulates PNPase activity, this vestigial site as an allosteric binding pocket that recognizes metal-free citrate
cytidyldadenylate dinucleoside
-
activates GDP polymerization
diphosphate
-
activation of PNPase RNA synthesis activity at very low concentrations of phosphate
dithiothreitol
-
similar activation as with 2-mercaptoethanol
interferon-beta
-
close association between interferon-beta induced upregulation of enzyme and c-myc downregulation
-
mercaptoethanol
-
20 mM, 3fold activation
phosphate
poly-L-lysine
Polyarginine
-
stimulation of poly(A) synthesis, phosphorolysis of poly(A) is inhibited
Polyornithine
-
stimulation of poly(A) synthesis, phosphorolysis of poly(A) is inhibited
putrescine
-
optimal polymerization activity requires polyamines, 2fold increase in activity at 30 mM
spermidine
spermine
additional information
-