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2.7.7.52: RNA uridylyltransferase

This is an abbreviated version!
For detailed information about RNA uridylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.52

Reaction

UTP
+
RNAn
=
diphosphate
 +
RNAn+3'-U

Synonyms

3' terminal uridylyl transferase, 3'-terminal uridylyl transferase 1, At2g45620, caffeine-induced death suppressor protein 1, Cid1, GLD2, HESO1, MEAT1, mitochondrial editosome-like complex associated TUTase 1, More, RET, RET1, RET2, RNA editing TUTase, RNA silencing protein 1, Rsp1, Tailor, TbMP57 TUTase, TbTUT3, TbTUT4, terminal RNA uridylyltransferase, terminal U transferase (TUTase), terminal uridylyl transferase, terminal uridylyltransferase, terminal uridylyltransferase Tailor, TNTase CG1091, TUT, TUT1, TUT2, TUT4, TUT7, TUTase, TUTase 1, TUTase 2, TUTase 3, TUTase 4, TUTase4, TUTase6, TUTase7, U6 snRNA-specific terminal uridylyltransferase, U6-TUTase, uridylyltransferase, terminal, URT1, UTP: RNA uridylyltransferase, UTP:RNA uridylyltransferase 1, Zcchc11, ZCCHC6

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.52 RNA uridylyltransferase

Cloned

Cloned on EC 2.7.7.52 - RNA uridylyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in Trypanosoma brucei cells strain 29.13
-
DNA sequence determination, editosomal enzyme, expression as His-tagged protein in Escherichia coli
-
DNA sequence determination, gene is located on chromosome 9, expression as His-tagged protein in Escherichia coli
-
DNA sequence determination, mitochondrial enzyme, expression as His-tagged protein in Escherichia coli
-
expressed in Escherichia coli BL-21 as a 6xHis tagged fusion protein
expressed in Escherichia coli BL21 Codon Plus cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Gold (DE3) cells
expressed in Sf9 insect cells
expression in Escherichia coli BL21(DE3) Codon Plus RIL, use of strains bearing additional tRNAs for expression of genes with G-rich codons is essential for RET1 expression
expression in HEK-293 cell
expression of RET2 in Leishmania tarentolae cells as cytosolic enzyme by removing the mitochondrial targeting signal sequence
-
for recombinant expression in Escherichia coli BL21 the RET2 gene lacking 21 codons at the 5' end, which correspond to the mitochondria importation signal, is cloned into pET15+vector to generate N-terminal 6 His-RET2 fusion protein
-
phylogenetic analysis, sequence comparison and signature motifs, overview
TbRET2 fused with the affinity TAP tag at the C-terminus using pLew79 vector in 29-13 cell line
-
TUT4, DNA and amino acid sequence determination and analysis, expression of N-terminally His-tagged wild-type and mutant TUT4 in Escherichia coli strain BL21(DE3)