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2.7.7.50: mRNA guanylyltransferase

This is an abbreviated version!
For detailed information about mRNA guanylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.50

Reaction

GTP
+
(5')ppPur-mRNA
=
diphosphate
 +
G(5')pppPur-mRNA

Synonyms

A103R, A103R protein, cap guanylyltransferase-methyltransferase, capping enzyme, capping enzyme guanylyltransferase, Ceg1, CET1, CmCeg1, D1 protein, GDP polyribonucleotidyltransferase, GlCeg1, GTase, GTP-RNA guanylyltransferase, GTP:RNA GTase, guanylyltransferase, guanylyltransferase mRNA capping, HCE, L protein, Mce1, messenger RNA guanylyltransferase, More, mRNA capping enzyme, mRNA guanylyl transferase, mRNA-cap, mRNA-capping enzyme, NAD-decapping enzyme, nsP1, NudC, PRNTase, protein lambda2, RNA capping enzyme, RNA guanylyltransferase, RNGTT, TbCgm1, TTM-type RTPase-GTase, VACWR106, VP3

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.50 mRNA guanylyltransferase

Molecular Weight

Molecular Weight on EC 2.7.7.50 - mRNA guanylyltransferase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
114200
-
LEF-4 subunit forming dimers in solution by weak ionic interactions, gel filtration
120000
-
copurifies with S-adenosylmethionine mRNA (guanine-7)-methyltransferase, sucrose density gradient centrifugation and gel filtration
127000
-
gel filtration, sucrose density gradient sedimentation
130000
-
gel filtration
140000
-
glycerol density gradient sedimentation
180000
-
glycerol density gradient sedimentation
26400
-
x * 95000, subunit containing the active site + x * 26400, transguanylyltransferase subunit, SDS-PAGE
28000
catalytically active residues 211-597, i.e. RNA guanylyltransferase domain, glycerol gradient sedimentation
31000
-
x * 95000 + x * 31000, SDS-PAGE
39000
-
x * 45000, alpha + x * 39000, beta, SDS-PAGE, probably alpha2beta2
42000
45000
-
x * 45000, alpha + x * 39000, beta, SDS-PAGE, probably alpha2beta2
46000
residues 1-210, i.e. RNA triphosphatase domain, glycerol gradient sedimentation
48500
-
sucrose density gradient sedimentation
52000
-
x * 80000, beta, RNA 5-triphosphatase + x * 52000, alpha, mRNA guanylyltransferase activity, SDS-PAGE
54000
-
LEF-4 subunit at 0.4 M KCl, gel filtration
59000
-
x * 59000, guanylyltransferase lacking 7-methyltransferase activity, SDS-PAGE
65000
67000
-
x * 67000, covalent enzyme-GMP complex, SDS-PAGE
68000
mRNA capping enzyme, glycerol density gradient sedimentation
69000
x * 69000, SDS-PAGE
80000
-
x * 80000, beta, RNA 5-triphosphatase + x * 52000, alpha, mRNA guanylyltransferase activity, SDS-PAGE
95000
additional information
-
the capping enzyme has 2 subunits: MW 95000 and 31000, the 95000 MW subunit of the vaccinia virus capping enzyme has guanylyltransferase activity, glycerol gradient centrifugation, the isolated 95000 MW guanylyltransferase can be converted to an active 60000 MW form in vitro by limited proteolysis with trypsin, the guanylyltransferase domain is localized to the amino two-thirds of the 95000 MW polypeptide