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2.7.7.50: mRNA guanylyltransferase

This is an abbreviated version!
For detailed information about mRNA guanylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.50

Reaction

GTP
+
(5')ppPur-mRNA
=
diphosphate
 +
G(5')pppPur-mRNA

Synonyms

A103R, A103R protein, cap guanylyltransferase-methyltransferase, capping enzyme, capping enzyme guanylyltransferase, Ceg1, CET1, CmCeg1, D1 protein, GDP polyribonucleotidyltransferase, GlCeg1, GTase, GTP-RNA guanylyltransferase, GTP:RNA GTase, guanylyltransferase, guanylyltransferase mRNA capping, HCE, L protein, Mce1, messenger RNA guanylyltransferase, More, mRNA capping enzyme, mRNA guanylyl transferase, mRNA-cap, mRNA-capping enzyme, NAD-decapping enzyme, nsP1, NudC, PRNTase, protein lambda2, RNA capping enzyme, RNA guanylyltransferase, RNGTT, TbCgm1, TTM-type RTPase-GTase, VACWR106, VP3

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.50 mRNA guanylyltransferase

Inhibitors

Inhibitors on EC 2.7.7.50 - mRNA guanylyltransferase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-amino-6-chloropurine-ribose triphosphate
-
3-[(5Z)-5-(2,3-dichlorobenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
3-[(5Z)-5-(2,4-dichlorobenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
3-[(5Z)-5-(4-tert-butylbenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]propanoic acid
Co2+
-
above 0.1 mM
diphosphate
hydrogen peroxide
-
inactivates the enzyme directly through irreversible oxidation of Cys126
mizoribine 5'-phosphate
compound can inhibit the formation of the RNA cap structure catalyzed by human capping enzyme. In the presence of mizoribine 5'-phosphate, the RNA 5'-triphosphatase activity appears to be relatively unaffected while the RNA guanylyltransferase activity is inhibited. Mizoribine 5'-phosphate is a non-competitive inhibitor that likely targets an allosteric site
Mn2+
-
can partially replace Mg2+ in activation, inhibits in presence of Mg2+
N-ethylmaleimide
-
-
NaCl
-
90% inhibition at 0.3 M
phosphate
ribavirin triphosphate
-
concentration 200fold more than the GTP input, 50% inhibition
sodium phosphonoformate
-
foscarnet, inhibition of the formation of the covalent intermediate at 5 mM
tetrapotassium diphosphate
-
inhibition of the formation of the covalent intermediate at 5 mM
Zn2+
-
above 10 mM
[(5Z)-4-oxo-5-(4-phenoxybenzylidene)-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
[(5Z)-5-(3,5-diiodo-2-methoxybenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
[(5Z)-5-[4-(benzyloxy)benzylidene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]acetic acid
additional information
-