2.7.7.49: RNA-directed DNA polymerase

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For detailed information about RNA-directed DNA polymerase, go to the full flat file.

Reaction

Synonyms

Bst DNA polymerase, CS5 pol, DNA nucleotidyltransferase (RNA-directed), DNA polymerase, DNA polymerase I, FeLV RT, FIV RT, FV Pol, FV reverse transcriptase, Gag-Pol, HBV-pol, HIV reverse transcriptase, HIV-1 M RT, HIV-1 O RT, HIV-1 R, HIV-1 reverse transcriptase, HIV-1 RT, HIV-reverse transcriptase, HIV-RT, human hepatitis B virus polymerase, human immunodeficiency virus type 1 reverse transcriptase, iScript enzyme, K4 polymerase, K4PolI, M-MuLV reverse transcriptase, MMLV RT, Moloney Murine leukemia virus reverse transcriptase, Moloney murine leukemia virus RT, MoMLV RT, More, MuLV RT, MX162-RT, MX65-RT, nucleoside reverse transcriptase, nucleotidyltransferase, deoxyribonucleate, RNA-dependent, p66 RT, P72, PFV RT, Pol, polymerase/reverse transcriptase, prototype foamy virus reverse transcriptase, R2-RT, reverse transcriptase, reverse transcriptase/RNA dependent DNA polymerase, reverse-transcriptase, revertase, RNA dependent DNA polymerase, RNA revertase, RNA-dependent DNA polymerase, RNA-instructed DNA polymerase, RT, SFV RT, simian foamy virus reverse transcriptase, SS RT, SuperScript I reverse transcriptase, Superscript II, SUPERSCRIPT II reverse transcriptase, T. Z05 pol, telomerase, telomerase catalytic subunit, telomerase reverse transcriptase, TERT, Tgo-Pol, xenotropic murine leukemia virus-related virus reverse transcriptase, XMRV RT, XNA reverse transcriptase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.7 Nucleotidyltransferases

                2.7.7.49 RNA-directed DNA polymerase

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Results	in table
22	Activating Compound
70257	AA Sequence
28	Application
1	CAS Registry Number
46	Cloned(Commentary)
9	Crystallization (Commentary)
47552	Disease/ Diagnostics
39	General Information
4	General Stability
311	IC50 Value
429	Inhibitors
31	kcat/KM [mM/s]
12	Ki Value [mM]
100	KM Value [mM]
14	Localization
79	Metals/Ions
37	Molecular Weight [Da]
66	Natural Substrates/ Products (Substrates)
4	Organic Solvent Stability
267	Organism
1098	PDB ID
26	pH Optimum
4	pH Range
2	pI Value
4	Posttranslational Modification
166	Protein Variants
48	Purification (Commentary)
1	Reaction
1	Reaction Type
262	Reference
34	Source Tissue
7	Specific Activity [micromol/min/mg]
10	Storage Stability
333	Substrates and Products (Substrate)
43	Subunits
221	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
2	Temperature Range [°C]
9	Temperature Stability [°C]
69	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.7.7.49 - RNA-directed DNA polymerase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-10°C, 50-70% loss of activity after 1 month

Rauscher murine leukemia virus

-

643415

-20°C, 50% glycerol, 0.2 mg/ml bovine serum albumin, no appreciable loss of activity after several months

Xenopus laevis

-

643421

-20°C, 50% glycerol, 2 mM dithioerythritol, less than 20% loss of activity after 2 months

Avian myeloblastosis virus

-

643418

-20°C, 50% glycerol, stable

Human T-cell lymphotropic virus/lymphadenopathy-associated virus

-

643432

-20°C, 50% v/v glycerol, purified enzyme retains activity for about 6 months, enzyme also retains activity when stored frozen at -70°C

Avian myeloblastosis virus

-

643420

-20°C, less than 10% loss of activity after 5 weeks

Avian myeloblastosis virus

-

643415

-70°C, 50% glycerol, stable for more than 12 months

Homo sapiens

-

643447

-70°C, stable for at least 6 months

Simian immunodeficiency virus

-

643446

-85 to -75°C, in frozen cells or tissues, at least one year, remains stable

Homo sapiens

-

691882

4°C, after 2 d the enzyme loses 20% of the poly(rA)*oligo(dT) activity, no activity loss is observed with poly(rC)*oligo(dG) activity

Simian immunodeficiency virus

-

643429