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2.7.7.49: RNA-directed DNA polymerase

This is an abbreviated version!
For detailed information about RNA-directed DNA polymerase, go to the full flat file.

Word Map on EC 2.7.7.49

Reaction

a 2'-deoxyribonucleoside 5'-triphosphate
+
DNAn
=
diphosphate
 +
DNAn+1

Synonyms

Bst DNA polymerase, CS5 pol, DNA nucleotidyltransferase (RNA-directed), DNA polymerase, DNA polymerase I, FeLV RT, FIV RT, FV Pol, FV reverse transcriptase, Gag-Pol, HBV-pol, HIV reverse transcriptase, HIV-1 M RT, HIV-1 O RT, HIV-1 R, HIV-1 reverse transcriptase, HIV-1 RT, HIV-reverse transcriptase, HIV-RT, human hepatitis B virus polymerase, human immunodeficiency virus type 1 reverse transcriptase, iScript enzyme, K4 polymerase, K4PolI, M-MuLV reverse transcriptase, MMLV RT, Moloney Murine leukemia virus reverse transcriptase, Moloney murine leukemia virus RT, MoMLV RT, More, MuLV RT, MX162-RT, MX65-RT, nucleoside reverse transcriptase, nucleotidyltransferase, deoxyribonucleate, RNA-dependent, p66 RT, P72, PFV RT, Pol, polymerase/reverse transcriptase, prototype foamy virus reverse transcriptase, R2-RT, reverse transcriptase, reverse transcriptase/RNA dependent DNA polymerase, reverse-transcriptase, revertase, RNA dependent DNA polymerase, RNA revertase, RNA-dependent DNA polymerase, RNA-instructed DNA polymerase, RT, SFV RT, simian foamy virus reverse transcriptase, SS RT, SuperScript I reverse transcriptase, Superscript II, SUPERSCRIPT II reverse transcriptase, T. Z05 pol, telomerase, telomerase catalytic subunit, telomerase reverse transcriptase, TERT, Tgo-Pol, xenotropic murine leukemia virus-related virus reverse transcriptase, XMRV RT, XNA reverse transcriptase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.49 RNA-directed DNA polymerase

KCat KM Value

KCat KM Value on EC 2.7.7.49 - RNA-directed DNA polymerase

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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.3 - 14.5
((2R,3S,5R)-3-hydroxy-5-(6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)tetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate
-
20 - 20.7
((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate
-
6.6 - 7.2
((2R,3S,5R)-5-(2-amino-6-(prop-2-yn-1-yloxy)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate
-
14.3
((2R,3S,5R)-5-(6-amino-8-(prop-2-yn-1-ylamino)-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate
-
pH 8.0, 37°C, RNA template
-
0.000005 - 0.048
3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate
0.000402 - 0.0015
3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate
0.000013 - 0.00023
3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate
0.00044
3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate
-
pH 7.5, 37°C, wild-type enzyme
0.044
3'-azido-2',3'-dideoxyadenosine triphosphate
-
pH 7.5, 37°C, wild-type enzyme
0.052 - 50.38
dATP
4.9
dCTP
-
pH 9.0, 37°C, recombinant mutant enzyme N483Q/S486Q/T539N/Y545Q/D547T/P548Q/A570Q/D578Q/A597T/W604R/S612N/V730L/R736Q/S739N/M747R
0.00102
dGTP
-
pH 7.5, 37°C, wild-type enzyme
1.8
dTTP
-
pH 9.0, 37°C, recombinant mutant enzyme N483Q/S486Q/T539N/Y545Q/D547T/P548Q/A570Q/D578Q/A597T/W604R/S612N/V730L/R736Q/S739N/M747R
14.89
N-cyclobutyladenosine-phosphonyl diphosphate
-
pH and temperature not specified in the publication, wild-type enzyme
7.16
p-tRNAHis
-
pH 7.5, 22°C
-
4.36 - 5.58
ppp-tRNAHis
-
0.039
ppp-tRNALeu
-
pH 7.5, 22°C, mutant enzyme D68A
-
0.0048 - 0.42
ppp-tRNAPhe
-
60.6
tenofovir diphosphate
-
pH and temperature not specified in the publication, wild-type enzyme