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2.7.7.48: RNA-directed RNA polymerase

This is an abbreviated version!
For detailed information about RNA-directed RNA polymerase, go to the full flat file.

Word Map on EC 2.7.7.48

Reaction

nucleoside triphosphate
+
RNAn
=
diphosphate
 +
RNAn+1

Synonyms

111 kDa protein, 180 kDa protein, 182 kDa protein, 183 kDa protein, 186 kDa protein, 216.5 kDa protein, 2A protein, 3CD protein, 3D pol, 3D polymerase, 3Dpol, 3Dpol-like protein, 3P complex, 69.6 kDa protein, core protein, core protein VP1, CVB3 RdRp, D-elp1, DENV 3 polymerase, DENV 3 RdRp, DENV RdRp, ego-1, ego-11, FluPol, FMDV 3D, GhRdRP, HC-J4 NS5BDELTA21, HCN NS5B protein, HCV NS5B, HCV NS5B polymerase, HCV RdRp, hepatitis C virus polymerase, HRV16 3Dpol, influenza polymerase PA, inner layer protein VP1, JEV NS5, JEV NS5 protein, JEV RdRp, jRdRp, L protein, L/P RdRP, large structural protein, M1 phosphoprotein, MOP1, More, NgRDR1, NIB, nonstructural phosphoprotein, nonstructural protein, nonstructural protein 12, nonstructural protein 5B, NS5, NS5 polymerase, NS5 protein, NS5 RdRp, NS5B, NS5B enzyme, NS5B polymerase, NS5B protein, NS5B RdRp, NS5B RNA-dependent RNA polymerase, NS5b-directed RNA polymerase, NS7, nsp12, nsp4, nucleocapsid phosphoprotein, nucleotidyltransferase, ribonucleate, RNA-dependent, ORF1, ORF1A, ORF1B, OsRDR6, P protein, P180, P3D, P66, P70, P88 protein, p92, PB1, PB1 proteins, PB2, PB2 proteins, Phage f2 replicase, phi6, phi6 polymerase, picornaviral 3D polymerase, plant-encoded RNA-dependent RNA polymerase 1, Pol, Pol IV, polymerase 3Dpol, polymerase acidic protein, polymerase basic 1 protein, polymerase L, protein 3Dpol, proteins PB1, proteins, PB 2, proteins, specific or class, lambda3, of reovirus, proteins, specific or class, PB 1, proteins, specific or class, PB 2, Q-beta replicase, Qbeta replicase, Qbeta-replicase, RDR, RDR1, RDR1a, RDR1b, RDR1c, RDR1c2, RDR2, RDR6, RDRP, replicase, phage f2, replicase, Qbeta, ribonucleic acid replicase, ribonucleic acid-dependent ribonucleate nucleotidyltransferase, ribonucleic acid-dependent ribonucleic acid polymerase, ribonucleic replicase, ribonucleic synthetase, RNA dependent RNA polymerase, RNA nucleotidyltransferase (RNA-directed), RNA polymerase, RNA polymerase IV, RNA replicase, RNA synthetase, RNA transcriptase, RNA-binding protein, RNA-dependent ribonucleate nucleotidyltransferase, RNA-dependent RNA polymerase, RNA-dependent RNA polymerase 1, RNA-Dependent RNA Polymerase 2, RNA-dependent RNA polymerase 6, RNA-dependent RNA polymerase NIb, RNA-dependent RNA polymerase NS5B, RNA-dependent RNA polymerases, RNA-dependent RNA replicase, RNA-dependent RNA-polymerase, RNA-directed RNA polymerase, RNA-directed RNA polymerase L, RNAdependent RNA polymerase, rrf-1, RRF-3, RrpC, RSV RdRp, SARS-CoV-2 RdRp, SARS-CoV-2 RNA polymerase, SARSCoV-2 polymerase, SARSCoV-2-core polymerase complex, self-priming RdRp, sigma NS protein, transcriptase, VP1, VP1 protein, WNV NS5

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.48 RNA-directed RNA polymerase

Cloned

Cloned on EC 2.7.7.48 - RNA-directed RNA polymerase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a codon-optimized cDNA sequence of ZIKV NS5 (amino acids 2522 to 3423; strain H/PF/2013) is synthesized de novo. The ZIKV NS5 polymerase portion is PCR amplified and cloned into a pMal-c5X vector under tac promoter control. Expression in Escherichia coli
a hexahistidine affinity-tagged NS5B fusion protein is expressed with recombinant baculoviruses in insect cells
-
bacterial production of the recombinant DENV-2 RdRp was difficult due to its low expression and solubility levels, recombinant expression of three different forms of DENV-2 RdRp as either N- or C-terminally His-tagged fusions, or without tag in Escherichia coli strains Rosetta (DE3) and BL21 (DE3). The presence of both the N- and C-terminal His-tag has a deleterious effect on polymerase activity, overview
C-terminal hydrophobic domain deletion mutant expressed in Escherichia coli
-
cloned and expressed in Escherichia coli with a C-terminal hexahistidine tag
-
co-expression of SARS-CoV-2 RNA-dependent RNA polymerase subunits with chaperones gives soluble expression in Escherichia coli
DNA and amino acid sequence determination and analysis, phylogenetic analysis based on the sequence of the RdRp whole domains, overview
DNA and amino acid sequence determination and analysis, sequence comparisons, NS5B genotyping, enzyme activities, and activity with ribavirin, overview. Expression of His-tagged enzyme variants in Escherichia coli
DNA sequence determination and analysis of hundreds of intronic regions encoding three or more RNA-dependent RNA polymerase-dependent sRNAs that are covered by dsRNA-seq (double-stranded RNA sequencing) reads, indicating that the intron-derived sRNAs are indeed generate from long double-stranded RNA precursors
-
enzymatically active full-length recombinant JEV NS5 protein with a hexahistidine tag at the N-terminus is expressed in Escherichia coli
-
enzyme RNA and amino acid sequence deermination and analysis, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain BL21(DE3), recombinant expression of soluble His6-tagged wild-type in High Five cells via the baculovirus expression system
-
expressed as a nonfusion protein in Escherichia coli
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) pCG1 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)/pCG1 cells
-
expressed in Escherichia coli coli BL21 (DE3) cells
-
expressed in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli XL1 Blue cells
-
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
-
expression in Escherichia coli using the N-terminal domain of Escherichia coli lysyl-tRNA synthetase as a solubility enhancer
-
expression in Escherichia coli, a mutant is generated as a result of the deletion of a trinucleotide in the N-terminal portion of the coding region, pT7-POL(TRP-). The protein expressed from this mutant lacks a tryptophan residue normally present at the fifth amino acid from the N-terminal glycine. This protein has no detectable enzymatic activity. Mutant pT7-POL(AvII), which lacks the C-terminal 53 amino acids of the normal protein is also inactive
-
expression in Sf9 cells infected with BVDV NS5B baculovirus
-
expression of ego-1 in the P19 EC cell line derived of murine embryonal carcinoma stem cells, co-expression with si RNA leads to gene silencing that is enhanced by ego-1, overview
-
expression of FLAG-tagged D-elp1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, and of His-tagged wild-type and mutant enzymes in Escherichia coli. Quantitative RT-PCR analysis for the expression levels of Dcr-2 and D-elp1 RNAs in S2 cells after depletion
-
expression of His-tagged subunits PA and PB1 in Escherichia coli
expression of NS5B in Huh7 cells
-
expression of recombinant enzyme chimeras by in vitro transcription
expression of SARS-CoV-2 nsp12 in Sf9 cells. Expression of SARS-CoV-2 nsp7 and nsp8 in Escherichia coli
expression of the recombinant enzyme in Escherichia coli
expression of wild-type and mutant enzymes in Escherichia coli
-
expression of wild-type and muztant enzymes in Escherichia coli strain BL21(DE3)
Cystovirus phi6
-
for recombinant expression in Spodoptera frugiperda Sf9 cells via baculovius transfection method, RSV RdRp is split into three pieces, since expression of the full protein cannot be achieved, expression as N-terminally His-tagged proteins or as GFP-fusion proteins
-
full-length enzyme and truncated enzyme forms resulting from deletions of 24, 36, 65 or 82 amino acid residues at the C terminal, with C-terminal hexahistidine tag, expression in Escherichia coli
-
fusion protein bearing a His6 tag at its C-terminus or at its N terminus is overexpressed in Escherichia coli
-
gene dr2, RT-PCR expression analysis
-
gene RdRP, phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, Agrobacterium-mediated virus infection of Solanum lycopersicum plants into cotyledons
gene rrpC, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis
gene StRDR1, phylogenetic analysis, enzyme expression analysis by quantitative reverse transcriptase PCR
identification and genomic analysis of 11956 non-transposon-related rice pseudogenes, most of which are from gene duplications, 145 of these pseudogenes potentially give rise to antisense small RNAs. 50% of these antisense RNAs are 24-nucleotides long, a feature often seen in plant repeat-associated small interfering RNAs produced by RNA-dependent RNA polymerase and Dicer-like protein 3
-
in vitro transcription of NS5B using SP6 RNA polymerase, and recombinant expression of His6-tagged NS5B and of untagged RDRP in Hep-G2 cells. In vitro cleavage of HCV core and NS5B mRNAs by DNAzyme in Hep-G2 cells is assessed by RT-PCR using RNA from cells co-transfected with cloned core or NS5B gene and its respective DNAzyme leading to reduction of NS5B protein activity in NDNAzyme co-transfected cells
N-terminal hexa-His-NS5BD21 (Con1 strain, GT1b with the C-terminal 21-amino acid membrane anchor domain deleted), expression in Escherichia coli
-
NgRDR1, DNA and amino acid sequence determination and analysis, semi-quantitative RT-PCR analysis, genetic structure, sequence comparisons, and phylogenetic tree
NS5B-hexahistidine fusion protein expressed with recombinant baculoviruses in insect Sf9 cells
-
overexpression in Escherichia coli
Cystovirus phi6
-
p92, in vitro translation using an extract of Nicotiana tabacum BY-2 cells, together with in vitro translated p33 formng th viral RNA replicase, overview
-
PmV1 genome organization analysis, nucleotide and amino acid sequence determination and analysis of sequence dsR2, phylogenetic analysis, recombinant expression in Escherichia coli strain TG1
putative RNA-dependent RNA polymerase (RdRp) domain cloned and expressed in Escherichia coli
-
RdRp gene encoded in the large RNA segment that contains a single ORF, nucleotide and amino acid sequence determination, phylogenetic analysis
RdRp gene, nucleotide and amino acid sequence determination and analysis, genome organization, detailed phylogenetic analysis and reclassification of SARS-CoVs, overview
-
RdRp gene, nucleotide and amino acid sequence determination and analysis, TSV genome organization, the Texas isolate has significant structural differences from the Hawaii isolate due to point mutation(s) in the RdRp gene in residues L1622, N1673, K1769, S1994, overview
-
RdRp, dsRNA 1 sequence determination and analysis via syntesis of the correspondant DNA sequences, phylogenetic analysis. Little single-stranded form of dsRNA 1 exists in the host Alternaria alternata, and most of the dsRNA 1 is present as genomic RNA rather than the replicative form of the ssRNA genome
-
RdRp, dsRNA sequence determination and analysis, genome structure and phylogenetic analysis, sequence comparisons
-
RdRp_2, genome organization analysis, and nucleotide and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of the His-tagged protein in Escherichia coli strain M15
-
recombinant enzyme P2 expression in Escherichia coli strain BL21 (DE3)
recombinant expression in Escherichia coli
-
recombinant expression of C-terminally His-tagged RNA-dependent RNA polymerase (RdRp) as the C-terminal domain of nonstructural protein 9 (nsp9) in Escherichia coli strain BL21 (DE3)
-
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli
recombinant expression of FLAG-tagged full-length enzyme in HEK-293 cells
recombinant expression of the viral His18-tagged enzyme components in Sf21AE and TN5 insect cells, establishing a single-round transcription system
-
recombinant full-length enzyme is expressed in insect cells
-
recombinant NV 3Dpol, as well as a 3Dpol active-site mutant are expressed in Escherichia coli
-
RNA genome organization, DNA and amino acid sequence determination, phylogenetic analysis
-
RNA genome organization, nucleotide and amino acid sequence determination, FgV3 is encoded in one of two geneome segments, phylogenetic analysis indicates that FgV3that FgV3 is closely related to members of the families Totiviriridae and Chrysoviridae, but is placed outside of their main clusters
RNA genome organization, nucleotide and amino acid sequence determination, segment FgV4 dsRNA-1 contains a single ORF, which has a conserved RdRp motif, phylogenetic analysis indicates that FgV4 forms a distinct clade with the family Partitiviridae
Fusarium graminearum dsRNA mycovirus 4
SARS-CoV-2 nsp12 polymerase and nsp7-nsp8 cofactors are expressed using the baculovirus and Escherichia coli expression systems, respectively. The three protein subunits were mixed in vitro to constitute the core polymerase complex
sequence comparisons, expression of mutant enzymes in BHK cells
-
sequencing of the single positive-strand RNA, monopartite, bicistronic genome of SINV-3 ORF 1 encoding the RNA-dependent RNA polymerase, and a helicase and protease, phylogenetic analysis
single positive-strand RNA sequence determination and analysis, sequence comparisons
single, positive-strand RNA sequence determination and analysis, ORF2b encodes the RNA dependent RNA polymerase, phylogenetic analysis
soluble Hepatitis C virus NS5B in the glutathione S-transferase-fused form NS5Bt which lacks the C-terminal 21 amino acid residues, expressed in Escherichia coli
-
the gene segment corresponding to the RdRp module corresponds to amino acid 272–905 of the NS5 protein, recombinant expression of the enzyme as N-terminally GST-tagged protein in Escherichia coli strain C41(DE3)
the pol domain of gene 1 is cloned and expressed in cells as a fusion with green fluorescent protein, termed Gpol
-
the SARS-CoV-2 nsp12 gene is codon-optimized for expression in insect cells. The SARS-CoV-2 nsp8 and nsp7 genes are codon-optimized for expression in Escherichia coli
wild-type and mutant enzymes, DNA and amino acid sequence determination and analysis