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2.7.7.43: N-acylneuraminate cytidylyltransferase

This is an abbreviated version!
For detailed information about N-acylneuraminate cytidylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.43

Reaction

CTP
+
N-acetylneuraminate
=
diphosphate
+
CMP-N-acylneuraminate

Synonyms

AaCSS, acylneuraminate cytidyltransferase, CMAS, Cmas1, Cmas2, CMP sialate pyrophosphorylase, CMP-N-acetylneuraminate synthase, CMP-N-acetylneuraminate synthetase, CMP-N-acetylneuraminic acid synthase, CMP-N-acetylneuraminic acid synthetase, CMP-N-acylneuraminic acid synthetase, CMP-NANA synthetase, CMP-Neu5Ac synthetase, CMP-NeuAc synthetase, CMP-NeuNAc synthetase, CMP-SA synthase, CMP-Sia synthetase, CMP-Sia-syn, CMP-sialate synthase, CMP-sialate synthetase, CMP-sialic acid synthetase, CMP-sialic acid synthetase (CSS), CMP-sialic synthetase, CMPsialate pyrophosphorylase, CMPsialate synthase, CNS, CSAS, CSS, cytidine 5'-monophosphate N-acetylneuraminic acid synthetase, cytidine 5'-monophospho-N-acetylneuraminic acid synthetase, cytidine 5'-monophosphosialic acid synthetase, cytidine 5-monophosphate N-acetylneuraminic acid synthetase, cytidine monophosphate N-acetylneuraminic acid synthetase, cytidine monophosphate sialic acid synthetase, cytidine monophosphate-N-acetylneuraminic acid synthetase, cytidine monophosphate-sialic acid synthetase, cytidine monophospho-sialic acid synthetase, cytidine monophosphoacetylneuraminic synthetase, cytidine monophosphosialate pyrophosphorylase, cytidine monophosphosialate synthetase, cytidyltransferase, acylneuraminate, cytidylyltransferase, acetylneuraminate, DmCSAS, DmCSS, hCSS, mCSS, N-Acetyl-neuraminic acid cytidylyltransferase, NmCSS, SaV CSS, sialate cytidylyltransferase, sialic acid cytidylyltransferase, TcCSS

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.43 N-acylneuraminate cytidylyltransferase

Molecular Weight

Molecular Weight on EC 2.7.7.43 - N-acylneuraminate cytidylyltransferase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
116000
-
gel filtration
135000
-
gel filtration
158000
-
gel filtration
160000
-
gel filtration
162000
-
gel filtration
164000
-
1 * 164000, SDS-PAGE
175800
tetramer, CMP-sialic acid synthetase, determined by gel filtration
18300
C-terminal domain of CMP-sialic acid synthetase, calculated
193400
gel filtration
236100
gel filtration
24800
-
gel filtration
24892
x * 24892, calculation from nucleotide sequence
24893
x * 24893, mass spectrometry
25400
-
gel filtration
25440
-
2 * 25440, dimer or larger oligomer, electrospray mass spectrometry
25600
-
2 * 25600, dimer or larger oligomer, SDS-PAGE
26400
N-terminal domain of CMP-sialic acid synthetase, calculated
27313
x * 27313, electrospray mass spectrometry
27500
-
predicted from cDNA
33700
x * 33700, SDS-PAGE
43000
44400
CMP-sialic acid synthetase, calculated
480000 - 1048000
when subjected to native PAGE, the recombinant AaCSS expressed in Escherichia coli forms large-sized aggregations (480 to over 1048 kDa), while those expressed in LEC29.Lec32 and S2 cells are soluble and smaller-sized complexes: 487 kDa and 644 kDa, respectively. The protein aggregation may be linked to loss of CSS activity, and caused by lack of protein modifications that are necessary for correct protein folding, such as protein N-glycosylation and/or a removal of N-terminal hydrophobic region containing the decapeptide sequence
48058
x * 48058, calculation from nucleotide sequence
48200
49000
-
SDS-PAGE
49600
4 * 49600, calculated
50000
-
x * 50000, SDS-PAGE
52000
-
3 * 52000, SDS-PAGE
55800
-
gel filtration
56600
dimer, N-terminal domain of CMP-sialic acid synthetase, determined by gel filtration
58000
-
2 * 58000, SDS-PAGE
60000
-
1 * 60000, SDS-PAGE
66000
SDS-PAGE, recombinant thioredoxin fusion protein
69000
87000
tetramer, C-terminal domain of CMP-sialic acid synthetase, determined by gel filtration
additional information
-
47000-74000 Da, gel filtration