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2.7.7.42: [glutamine synthetase] adenylyltransferase

This is an abbreviated version!
For detailed information about [glutamine synthetase] adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.42

Reaction

ATP
+
[glutamine synthetase]-L-tyrosine
=
diphosphate
+
[glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine

Synonyms

adenosine triphosphate:glutamine synthetase adenylyltransferase, adenylyltransferase, adenylyltransferase, glutamine synthetase, ATASE, ATP:glutamine synthetase adenylyltransferase, ATP:[L-glutamate:ammonia ligase (ADP-forming)] adenylyl transferase, GlnE, glutamine synthetase adenylyl transferase, glutamine synthetase adenylyltransferase, glutamine synthetase ATase, glutamine-synthetase adenylyltransferase, GS ATase, [glutamate-ammonia-ligase] adenylyltransferase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.42 [glutamine synthetase] adenylyltransferase

Cloned

Cloned on EC 2.7.7.42 - [glutamine synthetase] adenylyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in Escherichia coli
cloned in Escherichia coli DH5alpha, TG1 and JM109
-
co-expressed with Mycobacterium tuberculosis glutamine synthetase in Escherichia coli BL21 lambda(DE3) cells lacking glutamine synthetase and adenylyltransferase genes
overexpression
-
the plasmids pGEM-T Easy, pSUP202 and pRK2073 are used