2.7.7.41: phosphatidate cytidylyltransferase This is an abbreviated version! For detailed information about phosphatidate cytidylyltransferase, go to the full flat file .
Reaction
CTP +
phosphatidate =
diphosphate +
CDP-diacylglycerol
Synonyms CarS, CDP diacylglycerol synthase, CDP-DAG synthase, CDP-DG, CDP-DG synthase, CDP-DG synthetase, CDP-diacylglyceride synthetase, CDP-diacylglycerol synthase, CDP-diacylglycerol synthetase, CDP-diglyceride pyrophosphorylase, CDP-diglyceride synthetase, CDPdiglyceride pyrophosphorylase, CDS, CDS1, CDS2, CDS3, Cds4, Cds5, CdsA, CTP-diacylglycerol synthetase, CTP:1,2-diacylglycerophosphate-cytidyl transferase, CTP:phosphatidate cytidylyltransferase, cytidine 5'-diphosphate diacylglycerol synthase, cytidine diphosphoglyceride pyrophosphorylase, cytidinediphosphate diacylglycerol synthase, cytidylyltransferase, phosphatidate, DAG synthetase, More, phosphatidate cytidyltransferase, phosphatidate cytidyltransferase 2, phosphatidic acid cytidylyltransferase, SJAG_00426, Tam41, TAMM41, Tb927.7.220, TbCDS, YnbD
ECTree
Metals Ions
Metals Ions on EC 2.7.7.41 - phosphatidate cytidylyltransferase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ca2+
-
activates at low concentrations, decrease in activity at higher concentrations
Cu2+
about 30% of the activity with Mg2+
NH4+
Micrococcus cerificans
-
only about half of the activity observed with K+
Rb+
Micrococcus cerificans
-
only about half of the activity observed with K+
additional information
-
divalent cations are essential for activity
Co2+
-
activates at low concentrations, decrease in activity at higher concentrations
Co2+
about 80% of the activity with Mg2+
K+
-
stimulating effect increases with concentrations up to 75 mM
K+
Micrococcus cerificans
-
absolute requirement for
Mg2+
required, highest activity at 2-5 mM Mg2+ and 0.5% Triton X-100
Mg2+
required, highest activity at 25 mM Mg2+ and 0.5% Triton X-100
Mg2+
required, highest activity at 2-5 mM
Mg2+
required, highest activity at 25 mM
Mg2+
-
maximal activity with 3-6 mM. 33% inhibition at 25 mM
Mg2+
-
maximal activation, about 15fold, at 10 mM
Mg2+
-
divalent cation required, Mn2+ or Mg2+, maximal activity at 5 mM Mg2+. Maximal activity obtained with Mg2+ is about 1.2fold greater than that obtained with Mn2+
Mg2+
Micrococcus cerificans
-
stimulates, 3times more active than Mn2+, optimal activity at 10 mM
Mg2+
-
optimal concentration is 60 mM
Mg2+
-
50-60 mM required for optimal activity
Mg2+
-
the only divalent cation that supports activity
Mg2+
-
maximal activity at 20 mM Mg2+
Mg2+
required fro activity
Mg2+
highest activity with Mg2+, 2 mM used in assay conditions
Mg2+
-
maximal stimulation at 20 mM
Mn2+
-
1 mM, 70% of the maximal activity obtained with Mg2+
Mn2+
-
maximal activation, about 10fold, at 1 mM
Mn2+
-
divalent cation required, Mn2+ or Mg2+, maximal activity at 1 mM Mn2+
Mn2+
-
maximal activity at 18 mM
Mn2+
Micrococcus cerificans
-
stimulates
Mn2+
-
up to 10 mM, never more than 15% as effective as Mg2+