Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.7.3: pantetheine-phosphate adenylyltransferase

This is an abbreviated version!
For detailed information about pantetheine-phosphate adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.3

Reaction

ATP
+
pantetheine 4'-phosphate
=
diphosphate
+
3'-dephospho-CoA

Synonyms

3'-dephospho-CoA pyrophosphorylase, 4'-phosphopantetheine adenylyltransferase, 4-phosphopantetheine adenylyltransferase, CoaD, dephospho-CoA pyrophosphorylase, dephospho-coenzyme A pyrophosphorylase, Enterococcus faecalis PPAT, More, pantetheine phosphate adenylyltransferase, phosphopantetheine adenylyltransferase, PPAT

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.3 pantetheine-phosphate adenylyltransferase

Purification

Purification on EC 2.7.7.3 - pantetheine-phosphate adenylyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Co2 + affinity column chromatography
copurification as hexamer with 0.5 equivalents of CoA tightly bound per PPAT monomer
-
His Trap column chromatography, and Superdex 200 gel filtration
Ni-nitrilotriacetic acid-agarose affinity chromatography, gel filtration
Ni-NTA agarose column chromatography and Superdex 200 gel filtration
partial
Q Sepharose XL resin column chromatography, Q Sepharose HP resin column chromatography, and Sepharose S-200 gel filtration
recombinant enzyme PPAT from Escherichia coli strain BL21(DE3) to over 95% purity
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration
recombinant protein using His-tag
recombinant wild-type and mutant PPAT enzymes from Escherichia coli strain BL21 pLysS(DE3) to over 95% purity
recombinant wild-type and mutant PPAT enzymes from Escherichia coli strain BL21(DE3) to over 90% purity
-
the fusion protein was purified by amylose affinity chromatography
-
to homogenity
wild-type and recombinant enzyme to homogenity
-