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0.1 - 4.5
2-deoxy-2-amino-alpha-D-glucose 1-phosphate
0.08
3-deoxy-3-amino-alpha-D-glucose 1-phosphate
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
0.0003
3-deoxy-3-azido-alpha-D-glucose 1-phosphate
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
0.016 - 24
alpha-D-glucose 1-phosphate
0.01 - 0.55
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
0.1
2-deoxy-2-amino-alpha-D-glucose 1-phosphate
-
cosubstrate UTP, pH 7.5, temperature not specified in the publication
4.5
2-deoxy-2-amino-alpha-D-glucose 1-phosphate
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
0.016
alpha-D-glucose 1-phosphate
-
cosubstrate UTP, pH 7.5, temperature not specified in the publication
6.18
alpha-D-glucose 1-phosphate
-
cosubstrate UTP, pH 7.6, 37°C
7.3
alpha-D-glucose 1-phosphate
at pH 7.5 and 25°C
23.24
alpha-D-glucose 1-phosphate
cosubstrate: UTP, pH 7.5, 25°C
23.58
alpha-D-glucose 1-phosphate
cosubstrate: dTTP, pH 7.5, 25°C
24
alpha-D-glucose 1-phosphate
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication
0.06
ATP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.06
ATP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.48
ATP
-
mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.48
ATP
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.098
CTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.1
CTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
1.88
dATP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
1.88
dATP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
5.33
dATP
-
mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
5.33
dATP
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C
1.22
dCTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
1.22
dCTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
2.12
dGTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
2.12
dGTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
5
dGTP
-
mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
5
dGTP
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.56
dTDP-glucose
-
UgpG protein
1.35
dTDP-glucose
-
RmlA protein
0.37
dTTP
-
pH 7.6, 37°C
3.13
dTTP
-
mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
3.62
dTTP
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C
6.33
dTTP
-
mutant enzyme Q83S, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
6.33
dTTP
-
apparent value, mutant enzyme Q83S, in 100 mM Tris-HCl, pH 8.0, at 37°C
7.9
dTTP
at pH 7.5 and 25°C
15
dTTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
30.83
dTTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.14
GTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.14
GTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.21
GTP
-
mutant enzyme Q83D, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.21
GTP
-
apparent value, mutant enzyme Q83D, in 100 mM Tris-HCl, pH 8.0, at 37°C
2.1
UTP
-
pH 7.6, 37°C
24
UTP
-
wild type enzyme
41
UTP
-
wild type enzyme, apparent value, with 10 mM glucose-1-phosphate, 5 mM MgCl2, 10 units/ml inorganic pyrophosphatase, in 100 mM Tris-HCl, pH 8.0, at 37°C
41
UTP
-
apparent value, wild type enzyme, in 100 mM Tris-HCl, pH 8.0, at 37°C
0.01
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
-
cosubstrate UTP, pH 7.5, temperature not specified in the publication
0.55
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid
-
cosubstrate dTTP, pH 7.5, temperature not specified in the publication