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2.7.7.2: FAD synthase

This is an abbreviated version!
For detailed information about FAD synthase, go to the full flat file.

Word Map on EC 2.7.7.2

Reaction

ATP
+
FMN
=
diphosphate
 +
FAD

Synonyms

adenosine triphosphate-riboflavin mononucleotide transadenylase, adenosine triphosphate-riboflavine mononucleotide transadenylase, ATP-FMN adenylyltransferase, ATP:FMN adenylyl transferase, ATP:FMN adenylyltransferase, AtRibF1, AtRibF2, FAD pyrophosphorylase, FAD synthase, FAD synthetase, FAD synthetase isoform 1, FAD synthetase isoform 2, Fad1, FADS, FADS-6, FADS1, FADS2, FLAD1, flavin adenine dinucleotide synthetase, FMN adenylyltransferase, FMN pyrophosphorylase, FMN:ATP adenylyltransferase, FMNAT, lysZ, MJ1179, More, ribF, RibL, riboflavin adenine dinucleotide pyrophosphorylase, riboflavin mononucleotide adenylyltransferase, riboflavine adenine dinucleotide adenylyltransferase, SPAP_1083

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.2 FAD synthase

Molecular Weight

Molecular Weight on EC 2.7.7.2 - FAD synthase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
140000
-
gel filtration, purified enzyme can be separated into low molecular weight component of MW 140000 and MW 325000 high molecular weight component
16600
recombinant truncated C-terminal RF kinase domain, gel filtration
17000
2 * 17000, SDS-PAGE
28800
29200
325000
-
gel filtration, purified enzyme can be separated into low molecular weight component of MW 140000 and high molecular weight component of MW325000
33900
1 * 33900, SDS-PAGE and calculated
34000
-
x * 34000, His-tagged recombinant protein, SDS-PAGE
34180
wild-type, gel filtration
34200
34230
mutant RibC, gel filtration
35670
36000
36500
gel filtration
37120
-
electrospray mass spectrometry, Se-Met-enzyme, 36843.5 is the theoretical value for the native protein
37710
deduced from amino acid sequence
38000
53000
-
2 * 53000, SDS-PAGE
54190
calculated from amino acid sequence
54200
x * 54200 Da, predicted
55000
SDS-PAGE, T7-tagged enzyme
55840
calculated from amino acid sequence, T7-tagged enzyme
55844
x * 55000, SDS-PAGE, x * 55844, calculated
56537
x * 56537, isoform FADS2, calculated from amino acid sequence
57000
x * 57000, isoform FADS2, SDS-PAGE
60000
SDS-PAGE, isoform 2
63000
SDS-PAGE
65270
calculated from amino acid sequence
65300
x * 65300 Da, predicted
97000
-
gel filtration
additional information
the elution profile of full-length FADS shows two characteristic peaks at molecular weights corresponding to its monomeric and trimeric forms, while the tcRFK elutes as a single peak at an estimated molecular weight of 16.6 kDa, consistent with monomeric enzyme