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2.7.7.15: choline-phosphate cytidylyltransferase

This is an abbreviated version!
For detailed information about choline-phosphate cytidylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.15

Reaction

CTP
+
phosphocholine
=
diphosphate
 +
CDP-choline

Synonyms

AtCCT1, CCT, CCT-alpha, CCT1, CCT2, CCTalpha, CCTalpha236, CCTbeta, CCTbeta2, CCTbeta3, CDP-choline pyrophosphorylase, CDP-choline synthetase, choline phosphate cytidylyltransferase, choline-phosphate cytidylyltransferase A, choline-phosphate cytidylyltransferase-alpha, cholinephosphate cytidylyltransferase, CpCCT1, cpcT, CTalpha, CTbeta2, CTP-phosphocholine cytidylyltransferase, CTP:cholinephosphate cytidylyltransferase, CTP:phosphocholine cytidylyltransferase, CTP:phosphocholine cytidylyltransferase alpha, CTP:phosphocholine cytidylyltransferase alpha-isoform, CTP:phosphocholine cytidylyltransferase beta2, CTP:phosphocholine cytidylyltransferase beta3, CTP:phosphocholine cytidylyltransferase CCT, CTP:phosphocholine cytidylyltransferase-alpha, CTP:phosphocholine cytidylyltransferase-beta2, CTP:phosphocholine cytidylyltranspherase alpha, CTP:phosphorylcholine cytidylyltransferase, CTP:phosphorylcholine cytidylyltransferase CCT1, CTP:phosphorylcholine cytidylyltransferase CCT2, cytidine diphosphocholine pyrophosphorylase, cytidylyltransferase, cytidylyltransferase, choline phosphate, LmjF.18.1330, More, PCYT1A, PF3D7_1316600, PfCCT, phosphocholine cytidylyltransferase, phosphocholine cytidylyltransferase alpha, phosphorylcholine cytidylyltransferase, phosphorylcholine transferase, phosphorylcholine:CTP cytidylyltransferase

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.15 choline-phosphate cytidylyltransferase

Purification

Purification on EC 2.7.7.15 - choline-phosphate cytidylyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography, 2 forms: L-form and H-form, L-form: major species in fresh cytosol, H-form: consists of multiple copies of L-form
-
CM- and Blue-Sepharose chromatography, gel filtartion
deletion mutant which is truncated after residue 236, called CCTalpha236
-
dye-affinity chromatography with Green H-4G-Sepharose CL4B
-
homogeneity
mutant enzyme H630N
native and His-tag protein
-
Ni-NTA column chromatography
rapid purification method, 687fold purification
-
recombinant enzymes using His-tag
-
recombinant protein
-
recombinant truncated enzyme mutant CCTalpha236 by CM-sepharose chromatography
-
TALON Co2+ metal affinity chromatography
Talon metal affinity column chromatography
wild-type and truncated form
-
wild-type and truncated forms
-
wild-type enzyme and peptides after trypsin digestion
-